Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Peptide for promoting osteogenic differentiation or vascularization and use thereof

一种分化因子、活性成分的技术，应用在肽/蛋白质成分、心血管系统疾病、特定肽等方向，能够解决维生素D制剂昂贵效果、高花费、降血钙素制剂昂贵施用方法等问题，达到优异药物效应、低制造成本的效果

Active Publication Date: 2016-11-09
IND FOUND OF CHONNAM NAT UNIV +1
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these therapeutic agents have the following problems: bisphosphonate preparations have a low absorption rate and complicated administration methods, causing esophagitis; vitamin D preparations are expensive and their effects have not been confirmed; calcitonin preparations are expensive and administration methods are difficult; and calcium The formulation, although having fewer side effects, is effective in preventing rather than treating osteoporosis
Typically, recombinant proteins are more expensive to produce than low molecular weight compounds
On the other hand, due to protein properties, recombinant proteins have many limitations as pharmaceutical products from the viewpoint of physical properties and administration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Peptide for promoting osteogenic differentiation or vascularization and use thereof
  • Peptide for promoting osteogenic differentiation or vascularization and use thereof
  • Peptide for promoting osteogenic differentiation or vascularization and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0069] Synthesis of Pro-Pro-Arg-Ala-Cys-Pro-Thr-Ala-Arg-Ala-Leu-Cys-Glu-Ile-Arg (SEQ ID NO: 1)

[0070] Chlorotrityl chloride resin (CTL resin, Nova Biochem catalog number 01-64-0021) and 10 mL of dichloromethane (MC) were added in an amount of 700 mg to the reaction vessel and stirred for 3 minutes. In removing the solution, 10 mL of dimethylformamide (DMF) was added thereto, stirred for 3 minutes, and the solvent was removed. Add dichloromethane (10 mL), 200 mmol Fmoc-Arg(pbf)-OH (Bachem, Switzerland) and 400 mmol diisopropylethylamine (DIEA) to the reaction vessel, dissolve and react well with stirring while stirring for 1 h . Immediately after completion of the reaction, the resultant was washed, reacted with methanol and DIEA (ratio 2:1) dissolved in dichloromethane (DCM) for 10 min, and washed with excess DCM / DMF (ratio 1:1). After removing the solution, 10 mL of dimethylformamide (DMF) was added thereto, stirred for 3 minutes, and the solvent was removed. A deprotect...

experiment example 1

[0073] The structure of SEQ ID NO: 1 prepared in Example was confirmed by adding 15 amino acid sequences using the program provided by the online PEP-FOLD server. The result is as figure 2 shown.

[0074] from figure 2 It can be observed that the peptide of SEQ ID NO: 1 has an α-helical structure.

experiment example 2

[0076] In order to confirm the effect of BFP 5, which is the peptide of SEQ ID NO: 1 prepared in Example, on the activity of osteogenic differentiation, the following experiment was performed.

[0077] 1. Preparation of Medium for Osteoblasts and Osteogenic Differentiation

[0078] Mesenchymal stem cells cloned from Balb / c mouse bone marrow stromal cells (1x10 4 ) into 10% DMEM containing FBS, and in the atmosphere (about 37 ° C, 5% CO 2 ) for 3 days to prepare mesenchymal stem cells used as osteoblasts in this experiment. Osteogenic differentiation medium (hereinafter referred to as "ODM") consisted of DMEM containing 50 μg / mL ascorbic acid, 10 -8 M dexamethasone and 10mM β-glycerophosphate, etc.

[0079] 2. Measurement of Mineralization

[0080] Differentiation of mesenchymal stem cells into osteoblasts causes calcium accumulation. Here, the degree of calcium accumulation refers to the degree of differentiation of osteoblasts, and the degree of accumulation of calcium i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a protein effective for osteoblast differentiation and angiogenesis promotion and, more specifically, to a peptide and a use thereof.

Description

technical field [0001] The present invention relates to proteins effective in promoting osteogenic differentiation and vascularization, more specifically, peptides exhibiting activities of promoting osteogenic differentiation or vascularization, and uses thereof. Background technique [0002] Osteopenia is largely affected by changes in bone mass. The main factors affecting changes in bone mass include genetic factors, nutritional intake, hormonal changes, exercise, and lifestyle differences. Recently, it has been known that aging, lack of exercise, low-calcium diet, menopause, and ovariectomy cause bone mass loss and thus osteoporosis. [0003] Osteoporosis is a disease in which bones become very weak with the progression of symptoms of decreased calcium deposition in bone tissue, bone density becomes low and the medullary cavity widens, thereby easily causing bone-related The occurrence of disease (such as fracture) or even mild shock. [0004] Although there are indivi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/52A61K38/10A61P19/10A61P19/08A61P9/00A61P9/10
CPCC07K14/521A61K38/00C12N2501/998C07K14/522C12N5/0654A61P19/08A61P19/10A61P9/00A61P9/10
Inventor 尹择林金亨根宣钟根金枝贤姜周延
Owner IND FOUND OF CHONNAM NAT UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com