Western blot antibody incubation box

Abstract

The invention discloses a western blot antibody incubation box, and belongs to the field of laboratory instruments. The western blot antibody incubation box comprises a box body and a cover, wherein a flat plate is arranged inside the box body and maintains a certain height to the bottom surface, the upper surface of the flat plate has hydrophobicity, and rotation membranes are placed on the flat plate. According to the western blot antibody incubation box of the present invention, the rotation membranes and the antibody are placed on the hydrophobic flat plate, the antibody requirement amount is less, the contact of the antibody and the rotation membrane is uniform, the plurality of the rotation membranes can be arranged in one incubation, and no cross-contamination is generated; the groove on the bottom portion of the box body is filled with the water to maintain the moist state inside the box body so as to inhibit the volatilization of the antibody, wherein the volatilization of the water can be controlled by controlling the number of the groove for holding the water; and the whole structure is simple, the operation is convenient, the incubation experiment can be completed by using the plastic box body, the glass plate and the sealing membrane, the antibody requirement amount is less, the incubation efficiency is high, the recycling can be achieved, and the assurance is provided for the efficient and smooth performing of the experiment.

Description

technical field [0001] The invention relates to experimental equipment, in particular to a western blot antibody incubation box. Background technique [0002] In the existing western blot (Western blotting) process, most of them are carried out in airtight plastic bags or glass petri dishes. The plastic film needs to be sealed with a sealing machine, but because the plastic film is wetted by liquid, the sealing machine is difficult Seal it well. As a result, when the antibody is incubated on a shaker, the antibody diluent is easily leaked from the plastic seal, resulting in a waste of antibody. Secondly, the air bubbles in the plastic film must also be excluded during antibody incubation to avoid that when air bubbles exist, the antibody cannot fully contact the film, thereby affecting the experimental results. Using the existing method for antibody incubation, the steps are cumbersome, the operation is complicated, and the antibody waste is large. Contents of the invent...

AI Technical Summary

Problems solved by technology

[0002] In the existing western blot (Western blotting) process, most of them are carried out in airtight plastic bags or glass petri dishes. The plastic film needs to be sealed with a sealing machine, but because the plastic film is wetted by liquid, the sealing machine is difficult seal it up

As a result, when antibody incubation is performed on a shaker, the antibody diluent is easily leaked from the plastic seal, resulting in waste of antibodies

Secondly, the air bubbles in the plastic film must also be excluded during antibody incubation, to avoid that when air bubbles exist, the antibody cannot fully contact with the film, thus affecting the experimental results

Using the existing method for antibody incubation, the steps are cumbersome, the operation is complicated, and the waste of antibodies is large

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