Method for storing angelica dahurica seed at ultralow temperature
A technology of ultra-low temperature and seeds, applied in the fields of seed and rhizome treatment, botanical equipment and methods, fruit hanging devices, etc., can solve the problems affecting the income of medicinal plant growers, and achieve simple and easy storage methods with little impact and long storage time long effect
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[0031] The storage of embodiment 1, Angelica dahurica seeds
[0032] 1) The seeds of Angelica dahurica are dried, and the water content is adjusted to three different water contents of 5.34%, 7.70% and 9.43%, and then the seeds with different water contents are placed in airtight aluminum boxes for later use.
[0033] 2) The seeds of Angelica dahurica with different water contents in the above 1) are mixed with dimethyl sulfoxide, glycerin, polyethylene glycol 400 (PEG400) respectively as antifreeze protectant (the quality of Angelica dahurica seeds and antifreeze protectant is as shown in Table 1) shown), and then put them in plastic centrifuge tubes (with lids) and plastic ziplock bags (0.1mm thick), and store them in the following three ways:
[0034] Quick freezing: liquid nitrogen;
[0035] Slow freezing: refrigerator (5°C, 0.5h)-refrigerator (-20°C, 4h)-liquid nitrogen;
[0036] Slow freezing: refrigerator (5°C, 0.5h)-refrigerator (-20°C, 1h)-liquid nitrogen.
[0037]...
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