Microfluidic chip system and method for isothermal amplification and detection using the system

A microfluidic chip, isothermal technology, applied in laboratory containers, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problem of chip sealing method unfavorable on-site, portable use, etc., to prevent false positives. , low cost and simple system structure

Active Publication Date: 2019-08-16
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current chip sealing method is not conducive to field and portable use

Method used

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  • Microfluidic chip system and method for isothermal amplification and detection using the system
  • Microfluidic chip system and method for isothermal amplification and detection using the system
  • Microfluidic chip system and method for isothermal amplification and detection using the system

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Embodiment Construction

[0045]In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. The following description of the embodiments of the present invention with reference to the accompanying drawings is intended to explain the general inventive concept of the present invention, but should not be construed as a limitation of the present invention.

[0046] According to the general inventive concept of the present invention, a microfluidic chip system is provided, including a microfluidic chip, the microfluidic chip includes a structural sheet and a cover sheet, the structural sheet contains a reaction pool; the cover sheet is configured to cover the A sealed chamber is formed between the structural sheet and the structural sheet, the lower part of the sealed chamber is the reaction pool, and the cover sheet is ...

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Abstract

The invention discloses a micro-fluidic chip system which comprises a micro-fluidic chip and a temperature control module. The micro-fluidic chip comprises a substrate, a structural piece and a cover piece. The substrate is located at the bottom of the micro-fluidic chip. The structural piece is arranged on the substrate and comprises a reaction pool. The cover piece is configured to cover the structural piece, a sealing cavity is formed between the cover piece and the structural piece, the reaction pool is located under the sealing cavity and a paraffin section is cured on the side, forming the sealing cavity, of the cover piece. The temperature control module is configured to be fixed to the micro-fluidic chip and used for heating and carrying out temperature measurement and control on reactants in the reaction pool. In addition, the system may further comprise a detection module. The micro-fluidic chip system can achieve quantitative detection and qualitative detection for isothermal amplification.

Description

technical field [0001] The invention relates to the fields of life medical detection and disease diagnosis, in particular to a microfluidic chip system and a method for isothermal amplification and detection using the microfluidic chip system. Background technique [0002] PCR (Polymerase chain reaction) technology is the most typical nucleic acid amplification method and one of the most core technical means in molecular biology. It is cycled in units of amplification cycles, and each amplification cycle includes a denaturation stage (94 degrees, about 15 seconds), an annealing stage (60 degrees, about 30 seconds) and an extension stage (72 degrees, about 45 seconds), The amplification process is completed by dozens or more of such amplification cycles, which usually take 2-3 hours to complete. PCR technology has the characteristics of high sensitivity and good specificity, but it has long amplification time and complicated temperature control. [0003] Nucleic acid isothe...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B01L7/00C12M1/38C12M1/34C12M1/00C12Q1/6844
CPCB01L3/5027B01L7/52B01L2200/10B01L2300/1827C12Q1/6844C12Q2531/119C12Q2565/629
Inventor 陈兴崔大付张璐璐李亚亭徐春方任艳飞
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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