Micro-fluidic chip system and method for carrying out isothermal amplification and detection through same

A microfluidic chip and isothermal technology, applied to laboratory containers, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of unfavorable on-site and portable use of chip sealing methods, and prevent false positives , The detection instrument is compact and convenient for on-site use

Active Publication Date: 2016-11-23
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The current chip sealing method is not conducive to field and portable use

Method used

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  • Micro-fluidic chip system and method for carrying out isothermal amplification and detection through same
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  • Micro-fluidic chip system and method for carrying out isothermal amplification and detection through same

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Embodiment Construction

[0045] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings. The following description of the embodiments of the present invention with reference to the accompanying drawings is intended to explain the general inventive concept of the present invention, and should not be understood as a limitation to the present invention.

[0046] According to the overall inventive concept of the present invention, a microfluidic chip system is provided, including a microfluidic chip. The microfluidic chip includes a structure sheet and a cover sheet. The structure sheet includes a reaction cell; the cover sheet is configured to cover the On the structural sheet, a sealed cavity is formed between the structural sheet and the lower part of the sealed cavity is the reaction tank. The cover sheet is s...

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Abstract

The invention discloses a micro-fluidic chip system which comprises a micro-fluidic chip and a temperature control module. The micro-fluidic chip comprises a substrate, a structural piece and a cover piece. The substrate is located at the bottom of the micro-fluidic chip. The structural piece is arranged on the substrate and comprises a reaction pool. The cover piece is configured to cover the structural piece, a sealing cavity is formed between the cover piece and the structural piece, the reaction pool is located under the sealing cavity and a paraffin section is cured on the side, forming the sealing cavity, of the cover piece. The temperature control module is configured to be fixed to the micro-fluidic chip and used for heating and carrying out temperature measurement and control on reactants in the reaction pool. In addition, the system may further comprise a detection module. The micro-fluidic chip system can achieve quantitative detection and qualitative detection for isothermal amplification.

Description

Technical field [0001] The invention relates to the field of life medicine detection and disease diagnosis, in particular to a microfluidic chip system and a method for applying the microfluidic chip system for isothermal amplification and detection. Background technique [0002] PCR (Polymerase chain reaction) technology is one of the most typical nucleic acid amplification methods and one of the most core technical methods in molecular biology. It is cycled in units of amplification cycles. Each amplification cycle includes a denaturation phase (94 degrees, about 15 seconds), an annealing phase (60 degrees, about 30 seconds), and an extension phase (72 degrees, about 45 seconds). The amplification process is completed by dozens or more of these amplification cycles, which usually takes 2-3 hours to complete. PCR technology has the characteristics of high sensitivity and good specificity, but it has a long amplification time and complicated temperature control. [0003] The nucl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00B01L7/00C12M1/38C12M1/34C12M1/00C12Q1/68
CPCB01L3/5027B01L7/52B01L2200/10B01L2300/1827C12Q1/6844C12Q2531/119C12Q2565/629
Inventor 陈兴崔大付张璐璐李亚亭徐春方任艳飞
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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