A method for identifying Shatian pomelo in Rong County, Guangxi by chromatography
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A Shatian pomelo, chromatography technology, applied in the field of analytical chemistry chromatography, to achieve the effect of high separation efficiency, objective results, and simple operation
Active Publication Date: 2019-01-29
GUANGXI UNIV
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Problems solved by technology
At present, there is no report on the detection and identification of Shatian pomelo by gas chromatography or liquid chromatography
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Embodiment 1
[0051] Using gas chromatography and hydrogen flame ion detector to identify Shatian pomelo in Rongxian County, Guangxi, the specific operation steps are as follows:
[0052] 1. The initial temperature of the chromatographic column of the gas chromatograph is room temperature, and the temperature rises at a rate of 10°C / min to a temperature of 50-280°C. In order to meet the complete separation of the peaks of all components in the sample, the optimal analysis of the gas chromatogram is determined in combination with the boiling point of each substance. The conditions are as follows:
[0053] Gas Chromatography Analyzer Analysis Conditions
[0055] 2. Preparation of external standard solution: according to the mass fraction of external standard, weigh different mas...
Embodiment 2
[0058] The method for identifying Shatian pomelo in Rongxian County, Guangxi by using liquid chromatography-ultraviolet detector, the specific operation steps are as follows:
[0059] 1. The liquid chromatograph uses 254nm as the ultraviolet absorption wavelength, gradient elution, in order to meet the complete separation of peaks of all components in the sample, combined with the polarity of each substance, determine the optimal analysis conditions of liquid chromatography as follows:
[0060] Analysis conditions of liquid chromatography analyzer
[0061] Chromatographic column: Zorbax Eclipse XDB C18 250mm×4.6mm×5μm chromatographic column; mobile phase: A is aqueous phase, B is acetonitrile; elution flow rate: 1mL / min; column temperature: 30°C; injection volume: 10μL; gradient Elution program: 0-15min, A% (water): B% (acetonitrile) is 70:30, 15-20min, A% (water): B% (acetonitrile) is 40:60, 20-40min, A% (Water): B% (acetonitrile) is 40:60, 40 ~ 45min, A% (water): B% (aceton...
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Abstract
The invention discloses a method for identifying Shatian pomelo in Rong County of Guangxi by adopting chromatography. The method comprises: firstly, extracting chemical components in various parts, including external epidermis, internal epidermis, internal peel, pulp, seeds and the like, of the Shatian pomelo with acetonitrile; then detecting by adopting a gas chromatograph, a hydrogen flame ionization detector and a liquid-phase chromatograph-ultraviolet detector at the same time, and carrying out chromatograph qualitative analysis on the chemical components in the various parts of the Shatian pomeloe in Rong County of Guangxi, so as to accurately detect specific substances, including cnidiadin, limonene, alpha-linoleic acid, palmitic acid, sitosterol, oleamide, nootkatone and the like, in the Shatian pomeloe in Rong County of Guangxi; successfully establishing a Shatian pomelo specific substance chromatographic fingerprint spectrum. The method provided by the invention is simple in operation, accurate in quality determination and rapidness in detection, and is a brand-new rapid, accurate and simple Shatian pomelo origin place tracing and quality identification method.
Description
technical field [0001] The invention belongs to the field of analytical chemistry chromatography. Specifically, it is a method for identifying Shatian pomelo in Rongxian County, Guangxi by chromatography. The method uses gas chromatography and liquid chromatography to qualitatively analyze the chemical components in Shatian pomelo in Rongxian County, Guangxi. Background technique [0002] Pomelo (citrus junos tanaka), also known as Wendan, is a plant of the genus Pomelo in the citrus subfamily of Rutaceae. my country is the largest producer of pomelo in the world, and its output ranks second. Our country is rich in pomelo varieties and there are many varieties (strains). At present, the main varieties are Shatian pomelo, Guanxi honey pomelo, Yuhuan pomelo, etc., which account for about 80% of the country's pomelo cultivation area, with an average planting area of more than 3,700 hectares. The output exceeds 12,000 tons, mainly distributed in Fujian, Zhejiang, Taiwan, Guan...
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