Cell temperature sensor based on quantum dot film and its preparation method

Abstract

The invention provides a cell temperature sensor based on a quantum dot film which can measure a plurality of cells at the same time and can complete temperature measurement only by contact with the cells, and a preparation method, and belongs to the technical field of quantum dot film sensors. The temperature sensor includes a cover piece and a substrate, the cover piece is equipped with a microchannel structure, a quantum dot film is arranged on the substrate, the cover piece and the substrate are sealed, so that the microchannel structure and the quantum dot film form a closed cavity, and the cover piece is also provided with inlet / outlet holes of the cavity. The preparation method of the temperature sensor includes the following steps: 1. designing a microchannel structure, and manufacturing a mother board; 2. according to the mother board, manufacturing a cover piece with the designed microchannel structure, and punching inlet / outlet holes in the cover piece; 3. manufacturing a substrate, mixing a quantum dot solution and ultraviolet curing photoresist proportionally and then performing spin coating on the substrate, thereby preparing a quantum dot film; and 4. completing sealing-in of the cover piece and the substrate, so that the microchannel structure and the quantum dot film form a closed cavity.

Description

technical field [0001] The invention relates to a cell temperature sensor, in particular to a cell temperature sensor based on a quantum dot thin film and a preparation method thereof, and belongs to the technical field of quantum dot thin film sensors. Background technique [0002] Temperature measurement at the cell level is a difficult problem in the field of biomedicine. Its realization will not only enhance human understanding of cell physiology and pathology, but also contribute to new diagnostic and therapeutic methods for diseases (cancer, etc.) development. Traditional temperature measurement methods are divided into two categories: contact temperature measurement and non-contact temperature measurement, but it is difficult to achieve cell-level temperature measurement at the micro-nano scale. [0003] Quantum dot temperature sensor is a rising star in the fluorescent nanometer temperature sensor family, and it has been widely valued by scientific researchers at ho...

AI Technical Summary

Problems solved by technology

However, research on thermometry probes based on nanomaterials and molecular biomaterials is still in its infancy.

Among them, the nanothermocouple needs to be inserted into the cell when used, and the temperature measurement accuracy is high, but only one cell can be measured at a time, which is a destructive and lossy measurement; when the quantum dot temperature sensor is used, the cell also needs to swallow the quantum dots into the interior In order to measure temperature, the biocompatibility of quantum dots and other issues have brought many problems and obstacles to the in-depth research and application of quantum dots.

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