Real-time fluorescence PCR identification method, primer and probe of tiger derived component

Abstract

The invention discloses a real-time fluorescence PCR identification method, primer and probe of a tiger derived component. The method comprises the following steps: 1, extraction of DNA from a sample; 2, real-time fluorescence PCR amplification detection; and 3, result determination. The primer comprises an upstream primer and a downstream primer, the sequence of the upstream primer is 5'-CTTTATCTGTCTATACATGCAT-3', and the sequence of the downstream primer is 5'-TGTAGGAGCCGTAGTATATT-3'; and the sequence of the probe is 5'-FAM-ACGAGGAATATACTACGGCT-Eclipse-3'. Compared with common PCR methods, the method disclosed in the invention has the advantages of simplicity in operation, and high efficiency and accuracy in identification, can effectively identify tiger night soil, tiger hair and tiger bones, and can be used for field tracing investigation and protection of tigers. The primer and the probe have very high specificity and sensitivity, and can be used for protecting and researching types of tigers.

Description

technical field [0001] The invention relates to a species identification method, in particular to a real-time fluorescent PCR identification method for tiger-derived components and primers and probes thereof. Background technique [0002] Tigers are good hunters, but 90% of them miss their prey. It can be seen that under natural conditions, tigers will not cause the extinction of prey, let alone have any important impact on the number of prey communities. However, as humans continue to destroy the tiger's habitat, cut down plants, and kill the animals on which the tiger depends, the tiger's survival is threatened. In addition, widespread illegal hunting and illegal trade in tiger products in tiger range countries are also the reasons for the sharp decline in the number of wild tigers. Although law enforcement agencies have stepped up crackdowns, the number of illegal hunting that has been revealed may be only 1 / 5-1 / 6 of the actual hunting of tigers. In the process of law e...

AI Technical Summary

Problems solved by technology

Although law enforcement agencies have stepped up crackdowns, the number of illegal hunting that has been revealed may be only 1 / 5-1 / 6 of the actual tiger hunting

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