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Real-time fluorescence PCR identification method, primer and probe of tiger derived component

A real-time fluorescence, source component technology, which is used in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., to achieve high specificity and sensitivity, efficient and accurate identification, and easy operation.

Inactive Publication Date: 2017-03-29
ZHEJIANG ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although law enforcement agencies have stepped up crackdowns, the number of illegal hunting that has been revealed may be only 1 / 5-1 / 6 of the actual tiger hunting

Method used

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  • Real-time fluorescence PCR identification method, primer and probe of tiger derived component
  • Real-time fluorescence PCR identification method, primer and probe of tiger derived component
  • Real-time fluorescence PCR identification method, primer and probe of tiger derived component

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Embodiment

[0025] 1. Design of primers and probes

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Abstract

The invention discloses a real-time fluorescence PCR identification method, primer and probe of a tiger derived component. The method comprises the following steps: 1, extraction of DNA from a sample; 2, real-time fluorescence PCR amplification detection; and 3, result determination. The primer comprises an upstream primer and a downstream primer, the sequence of the upstream primer is 5'-CTTTATCTGTCTATACATGCAT-3', and the sequence of the downstream primer is 5'-TGTAGGAGCCGTAGTATATT-3'; and the sequence of the probe is 5'-FAM-ACGAGGAATATACTACGGCT-Eclipse-3'. Compared with common PCR methods, the method disclosed in the invention has the advantages of simplicity in operation, and high efficiency and accuracy in identification, can effectively identify tiger night soil, tiger hair and tiger bones, and can be used for field tracing investigation and protection of tigers. The primer and the probe have very high specificity and sensitivity, and can be used for protecting and researching types of tigers.

Description

technical field [0001] The invention relates to a species identification method, in particular to a real-time fluorescent PCR identification method for tiger-derived components and primers and probes thereof. Background technique [0002] Tigers are good hunters, but 90% of them miss their prey. It can be seen that under natural conditions, tigers will not cause the extinction of prey, let alone have any important impact on the number of prey communities. However, as humans continue to destroy the tiger's habitat, cut down plants, and kill the animals on which the tiger depends, the tiger's survival is threatened. In addition, widespread illegal hunting and illegal trade in tiger products in tiger range countries are also the reasons for the sharp decline in the number of wild tigers. Although law enforcement agencies have stepped up crackdowns, the number of illegal hunting that has been revealed may be only 1 / 5-1 / 6 of the actual hunting of tigers. In the process of law e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6851C12Q1/6888C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 吴姗章惠娟张晓峰杨兰花
Owner ZHEJIANG ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
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