50results about How to "Effective research" patented technology

Preferred systems for assisting clearance of an acutely thrombosed artery substantially surrounded by boney external body surfaces which are resistant to deformative displacement relative to the thrombosed artery by the application of external percussive force are described. The method consists of applying targeted, localized, non-invasive, high infrasonic to low sonic frequency vibratory percussion with a serial impact frequency much greater than the pulse rate of a patient being treated, the percussion directed towards a remote, preferably superficial “target vessel” residing palpably close to the skin surface. Marked vessel deformations with resultant blood pressure and flow fluctuations are thereby induced by the percussion within the target vessel which propagate to the acutely thrombosed artery to provide localized agitation and turbulence to assist thrombolytic and/or IV microbubble delivery and effectiveness in facilitating reperfusion. Preferred apparatus for treatment of ST elevation myocardial infarction, acute ischemic stroke and acute pulmonary embolus are presented.

The invention discloses an experimental apparatus for a non-constant debris flow. The experimental apparatus comprises a water storage tank, a hopper placed with debris, a frame for installing the hopper, a tank which is obliquely arranged and has an adjustable oblique angle, and an aggregate pool for accommodating the materials from the tank, wherein the inlet of the tank is higher than the outlet of the tank, the water outlet of the water storage tank is connected with the inlet of the tank, the water in the water storage tank and the debris in the hopper converge at the inlet of the tank to form the experimental debris flow fluid; then the debris flow fluid flows into the aggregate pool along the tank; and the bottom plate of the tank is provided with a pressure sensor, and the side plates of the tank are transparent. The invention has the advantages that: the experimental apparatus is simple in operation and can be used for effectively studying the movement characteristics of the non-constant debris flow.

A tracing method for the intermedullary filling stem cells includes such steps as using transfectant to modify superparamagnetic iron oxide to make it carry positive charges, using it to mark the cultured intermedullary filling stem cells, inoculating said cells onto scaffold, transplanting it in the body of animal, and using NMR imaging instrument for tracing the cells in animal body.

The invention discloses an SON (Silicon on Nothing) deformation mechanism study method based on a phase field model at a high temperature. The method comprises the following steps of 1, putting a sample, i.e., an SON material at a certain temperature so that the sample becomes soft, and then making the sample into a U-shaped cylindrical hole shape; 2, putting the SON material into a high-temperature environment to be processed for several minutes for obtaining the SON form change state in different time periods; and 3, building a system model of the SON at the high temperature. According to the method, the SON deformation mechanism at the high temperature is studied by the method of integrating the experiment study and the simulation model for the first time. The SON forming process is controlled by using the high-temperature atomic diffusion motion; through the simulation model, the SON forming change can be more directly observed; and thus, a novel idea for processing the SON is provided.

The invention relates to the modeling technology, and aims to provide a modeling method of a virtual synchronous generator taking an excitation circuit into consideration. Based on a three-phase bridge inverter topological structure, a power loop of the virtual synchronous generator is analyzed by neglecting an inner ring voltage and a current loop in a control system; by referring to a flux linkage equation, a voltage equation, a torque equation, a power equation and a motion equation of a traditional synchronous machine, a simplified motor model is proposed by further neglecting a derivativeterm and a zero sequence term in the model; in the model, the excitation current is calculated in a virtual mode by utilizing a reactive power deviation value and an excitation coefficient, and an excitation circuit is constructed; and the obtained motor model is expressed in a small signal disturbance mode, so that a low-order small signal model of the virtual synchronous generator taking the excitation circuit into consideration is obtained. According to the method, the grid-connected topological structure of the common inverter does not need to be changed, and a common grid-connected inverter which is mature and wide in application can be used for carrying out model research, so that the model test is completed under the condition that additional hardware cost is not increased.

The present disclosure provides methods for genetically modifying lymphocytes and methods for performing adoptive cellular therapy that include transducing T cells and/or NK cells. The methods can include inhibitory RNA molecule(s) and/or engineered signaling polypeptides that can include a lymphoproliferative element, and/or a chimeric antigen receptor (CAR), for example a microenvironment restricted biologic CAR (MRB-CAR). Additional elements of such engineered signaling polypeptides are provided herein, such as those that drive proliferation and regulatory elements therefor, as well as replication incompetent recombinant retroviral particles and packaging cell lines and methods of making the same. Numerous elements and methods for regulating transduced and/or genetically modified T cells and/or NK cells are provided, such as, for example, those including riboswitches, MRB-CARs, recognition domains, and/or pH-modulating agents.

The invention discloses a multi-parking-lot multi-vehicle-model vehicle path planning method. The method comprises the following steps: 1) establishing an objective function by taking the lowest totalcost of vehicle distribution as an objective; 2) encoding and redefining an operation mode; 3) initializing a population; 4) calculating the fitness of the artificial fish, and recording the optimalindividual; 5) evaluating each fish, selecting the behavior of each fish at the same time, and selecting the behavior with the highest fitness improvement to execute; 6) calculating the fitness of theobtained new population, and updating the optimal solution in the population; 7) performing annealing search on the optimal solution of the population; 8) ending the primary iteration; 9) judging whether a termination condition is met or not, and if so, executing the step 10; otherwise, returning to execute the step 5, and carrying out a new round of iteration; and 10) decoding the finally recorded optimal individual, calculating the reciprocal of the fitness of the optimal individual, i.e., the value of the target function, and after decoding, taking the client between two same vehicle numbers in the obtained decoding sequence as the distribution route of the vehicle, and outputting an optimal scheme when the unused vehicle does not appear in the decoding sequence.

The invention discloses a haze particle formation simulator which comprises a combustion furnace, a control console, a growth simulation chamber, a light shield, a sucking pump, a flow meter, a three-way valve with a stop valve, a haze particle collector and an air purifier, wherein the growth simulation chamber is fixed on the control console; the combustion furnace, the growth simulation chamberand the air purifier are connected into a system by the sucking pump; the three-way valve with the stop valve realizes independent operation of a smoke dust source supply channel and a haze particlecollection channel; multiple feeding holes and an exhaust valve are formed in the growth simulation chamber, and the growth simulation chamber is equipped with temperature, humidity, airspeed and light intensity detection sensors as well as a light source, a fan and a supercharging balloon; and a temperature control device, a humidifier and a pressure gage are arranged in the control console. By detecting and regulating the temperature, pressure, humidity, airspeed and light intensity in the growth simulation chamber, the outdoor environment formed by haze is simulated, a research is conductedon formation of single haze particles or a multi-haze particle mixing system, and the haze particle formation simulator has obvious value on treatment of the atmospheric environment.

The invention relates to a method for implementing strong vivo transplantation of human induced pluripotent stem cell-derived hemopoietic stem progenitor cells. The method is induced by an MLL-AF4 single factor and has the advantages that the method is simple, convenient and efficient, human iPS (induced pluripotent stem) cells can be induced into HSC (hemopoietic stem cells) with strong vivo transplantation effects and multi-strain hemopoietic reconstruction, and many difficulties in the field are broken through. The method mainly has the advantages that the number of factors needing to be added is greatly decreased by induction with the single factor, experimental operation can be remarkably simplified, experimental efficiency is improved, the risk of gene mutation possibly induced by slow virus mediated genome insertion is avoided by a plasmid-mediated inducing method, vivo transplantation efficiency reaches up to 20% or more, medullary system bias of iPSC-HSPC in vivo is corrected,and a medullary system, a lymphoid system and an erythroid can be comprehensively reconstructed in vivo.

The invention relates to a biological specimen tomography cutting and imaging device and the working method thereof. The device comprises a material feeding module, a cutting module, a camera module, a controller and a computer wherein the cutting module and the camera module are both connected with the controller, the material feeding module is connected with the cutting module, and the control module is connected with the computer. The material feeding module is used to transport the biological specimen in need of cutting and controls the transportation speed; the cutting module is used to cut the biological specimen aided by infrared rays; the camera module is used to photograph the cut tomography of the biological specimen; the controller is used to control the size of the cut biological specimen, to control the camera module to photograph the cut specimen and to save the photographed images in a storage device; and the computer conducts three-dimensional reconstruction to the cut biological specimen. According to the method of the invention, through the technology of using the thinnest possible cutters, the biological specimen could be reconstructed in three dimensions by a computer so that a three-dimensional model for the biological specimen could be obtained accurately.

The invention provides a random structural plane network contained rock mass model 3D printing and mechanical parameter acquisition method. The method comprises the steps of sampling a natural rock mass containing a random structural plane network, obtaining a three-dimensional space scanning image or a three-dimensional space distribution characteristic of the structural plane network, and obtaining mechanical parameter values of the structural plane network; and taking rock sampled at the same place of the natural rock mass for 3D printing to obtain a copy model, comparing mechanical parameter values of the copy model and the natural rock mass sample, adjusting a printing scheme until the copy model and the natural rock mass sample have similar mechanical properties, printing a plurality of copy models according to the adjusted printing scheme, carrying out various physical and mechanical tests, and obtaining mechanical parameters. According to the method, the natural rock mass structure can be accurately simulated, the mechanical properties of the rock mass containing the random structural plane network can be effectively researched, meanwhile, the random structural plane network of the rock mass can be repeatedly reproduced, multiple groups of tests can be conveniently carried out to obtain different mechanical parameters of the model, and the method has important significance on research on the mechanical property of the rock mass containing the random structural plane network.

The invention discloses a method for displaying and collecting distribution images of motor end plates inside levator ani muscles. The method comprises the following steps: dyeing a motor end plate inside a levator ani muscle sample by a cholinesterase mass dyeing process; preparing a hollow transparent silica gel model, and placing a background light source inside the silica gel model; placing the levator ani muscle sample with the dyed motor end plate on an outer surface of the silica gel model; acquiring the three-dimensional shape images distributed by the motor end plates inside the levator ani muscles; and placing the sample under a dissecting microscope so as to carry out microdissection and image acquisition. With the adoption of the method for displaying and collecting distribution images of motor end plates inside the levator ani muscles, the distribution images of the motor end plates inside levator ani muscles can be dyed, the purpose of completely displaying the distribution state of the motor end plates inside the levator ani muscles can be achieved under mapping of the background light source, and the effective research images for moving distribution rules of the distribution images of the motor end plates inside levator ani muscles are provided.

The present invention provides a path planning method for an electric vehicle with a time window. The method comprises the steps of (1) setting relevant parameter variables, and establishing a model;2) designing a coding mode and redefining a related operation mode; 3) initializing a population, and initializing a central position and a vaccine; 4) grouping the population, executing a search modefor one part, turning to the step 5, executing a tracking mode for the other part, and turning to the step 6; 5) executing a search mode; 6) executing a tracking mode; 7) updating fitness of population individuals; 8) updating the central position of the population; 9) performing simulated annealing search on the population optimal solution, 10) finishing one iteration and updating the iterationfrequency g=g+1; 11) judging whether a termination condition is met or not, if so, executing the step 12), otherwise, returning to execute the step 4) and performing a new round of iteration, and 12)decoding the recorded optimal solution and outputting an optimal scheme.

The invention discloses a real-time fluorescence PCR identification method, primer and probe of a tiger derived component. The method comprises the following steps: 1, extraction of DNA from a sample; 2, real-time fluorescence PCR amplification detection; and 3, result determination. The primer comprises an upstream primer and a downstream primer, the sequence of the upstream primer is 5'-CTTTATCTGTCTATACATGCAT-3', and the sequence of the downstream primer is 5'-TGTAGGAGCCGTAGTATATT-3'; and the sequence of the probe is 5'-FAM-ACGAGGAATATACTACGGCT-Eclipse-3'. Compared with common PCR methods, the method disclosed in the invention has the advantages of simplicity in operation, and high efficiency and accuracy in identification, can effectively identify tiger night soil, tiger hair and tiger bones, and can be used for field tracing investigation and protection of tigers. The primer and the probe have very high specificity and sensitivity, and can be used for protecting and researching types of tigers.

The invention relates to a flexible layered mud sampler. The flexible layered mud sampler comprises a mud sampling tube which mainly comprises a first mud sampling tube, a second mud sampling tube and a third mud sampling tube, wherein the first mud sampling tube is hermetically arranged in the second mud sampling tube, the second mud sampling tube is hermetically arranged in the third mud sampling tube, the first mud sampling tube is provided with a piston, a first mud sampling opening is formed on the end part of the first mud sampling tube, a second mud sampling opening is formed on the end part of the second mud sampling tube, and a third mud sampling opening is formed on the end part of the third mud sampling tube. The mud sampler is simple in structure and can retract together in the carrying process, so that the mud sampler is convenient to carry. Moreover, when the mud sampler is used for sampling mud, the layering accuracy of a mud sample can be ensured, and composition and origin of the mud sample and biocenosis in the mud sample can be effectively researched.

The invention discloses an information sharing-based dangerous environment escape and rescue system. The system comprises a plurality of mobile terminals, a safety monitoring terminal and a cloud server, wherein the mobile terminals and the safety monitoring terminal are in two-way communication through a local area network, and the safety monitoring terminal and the cloud server are in two-way communication through the Internet; the mobile terminal comprises a voice talkback module, a gas detection module, a communication module, a positioning module and an anti-falling sensor module; and the safety monitoring terminal comprises a wind direction monitoring module and a wind speed monitoring module. According to the system, when the gas detection module detects dangerous gas, the voice talkback module in the mobile terminal is matched with the communication module and the positioning module to display alarm information sent by other mobile terminals and the safety monitoring terminal in the same area; the safety monitoring terminal transmits real-time wind direction and the wind speed to the mobile terminal through the wind direction monitoring module and the wind speed monitoring module via the local area network; and a purpose of timely escaping and rescuing the staff is achieved.

The invention relates to a method for extracting high-purity lithium salt from lithium-containing brine, which belongs to the technical field of liquid-liquid extraction. The method comprises the following steps: after the extraction step and before the reverse extraction step, an exchanging step is introduced to exchange lithium and an impurity element, wherein an exchanging solution used in theexchanging step is a lithium salt solution. By virtue of the method of the invention, the high-purity lithium salt with the purity greater than 99.9 percent can be obtained, the reflux extraction theory is further applied to the lithium extraction of salt lake, an appropriate theoretical stage can be designed on purpose by virtue of calculation, the problems that the process is instable, the product purity is not high, the normal continuous production cannot be realized and the like can be solved, the scientific research and production for extracting the lithium from the brine can be effectively instructed, and the application prospect is wide.