Method for displaying and collecting distribution images of motor end plates inside levator ani muscles

Abstract

The invention discloses a method for displaying and collecting distribution images of motor end plates inside levator ani muscles. The method comprises the following steps: dyeing a motor end plate inside a levator ani muscle sample by a cholinesterase mass dyeing process; preparing a hollow transparent silica gel model, and placing a background light source inside the silica gel model; placing the levator ani muscle sample with the dyed motor end plate on an outer surface of the silica gel model; acquiring the three-dimensional shape images distributed by the motor end plates inside the levator ani muscles; and placing the sample under a dissecting microscope so as to carry out microdissection and image acquisition. With the adoption of the method for displaying and collecting distribution images of motor end plates inside the levator ani muscles, the distribution images of the motor end plates inside levator ani muscles can be dyed, the purpose of completely displaying the distribution state of the motor end plates inside the levator ani muscles can be achieved under mapping of the background light source, and the effective research images for moving distribution rules of the distribution images of the motor end plates inside levator ani muscles are provided.

Description

Technical field [0001] The invention relates to a dyeing technique of the motor endplate, in particular to a method for displaying and collecting images of the motor endplate distribution in the levator ani muscle. Background technique [0002] Epidemiological studies have found that 15% of adults have chronic pelvic pain, and 80% of patients with chronic pelvic pain suffer from levator spasm. In animal experiments and clinical studies, it has been found that injecting botulinum toxin into the area of ​​the intramuscular motor endplate can improve the therapeutic effect of muscle spasms and dystonia caused by abnormal motor neuron function. Therefore, clarifying the distribution of the motor endplate in the levator ani can help guide the clinical application of botulinum toxin directly injected into the distribution area of ​​the motor endplate in the levator ani muscle to relax muscle spasm, effectively treat chronic pelvic pain, and reduce side effects the goal of. [0003] At ...

AI Technical Summary

Problems solved by technology

The reasons include: 1. The composition of the levator ani is complex; 2. The location of the levator ani is special; 3. The levator ani is closely related to internal organs such as the urethra, vagina, rectum and anal canal, and most obstetrics and gynecology, urology and anorectal Clinical scholars of surgery mistakenly regard the levator ani muscle as a visceral muscle, rather than a part of skeletal muscle; 4. Clinical scholars of related majors lack professional knowledge of anatomy and histopathology on the staining methods of motor endplates, while classic There are few studies on levator ani muscle and motor end plate in basic anatomy research

[0004] Although cholinesterase staining is a classic method for studying the distribution of motor endplates in skeletal muscles, there is no report on the distribution of motor endplates in the levator ani muscle using cholinesterase staining.