Three-dimensional scaffold applied to cell culture and preparation method thereof

A three-dimensional scaffold, cell culture technology, applied in the field of cell culture, can solve problems such as speeding up wound repair

Inactive Publication Date: 2017-03-22
TIANJIN WEIKAI BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Because chitosan and collagen can be cross-linked and bonded to obtain a composite material, the composite material is used in biological dressings, skin scaffold materials, etc. due to its good biocompatibility, which can promote cell growth and accelerate wound repair. The application in cell culture has not been reported, especially for three-dimensional cell culture, it is urgent to develop a scaffold material with good biocompatibility, good stability and microcirculation characteristics

Method used

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  • Three-dimensional scaffold applied to cell culture and preparation method thereof
  • Three-dimensional scaffold applied to cell culture and preparation method thereof
  • Three-dimensional scaffold applied to cell culture and preparation method thereof

Examples

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preparation example Construction

[0044]On the other hand, an embodiment of the present invention provides a method for preparing the three-dimensional scaffold as described above, including:

[0045] Step 1) preparing the acetic acid solution of the collagen-chitosan;

[0046] Step 2) degassing the acetic acid solution of collagen-chitosan and pouring it into a container, freeze-drying, and cutting into a preset shape to obtain a porous scaffold material;

[0047] Alternatively, a cell culture well plate with a plurality of holes is selected, wherein the cell culture well plate satisfies the following conditions: the cell culture well plate includes surrounding wells arranged on the edge and a central well surrounded by the surrounding wells at the center ;

[0048] The acetic acid solution of collagen-chitosan is defoamed and injected into the central hole to a preset height, and water is injected into the surrounding hole to a preset height, and then freeze-dried to obtain a porous scaffold material.

[0...

Embodiment 1

[0080] For convenience of description, the three-dimensional scaffold prepared in Example 1 is designated as A.

[0081] The specific preparation method is as follows:

[0082] 1) prepare the acetic acid solution of collagen-chitosan, wherein, the concentration of collagen is 0.1g / L, and the concentration of chitosan is 1g / L;

[0083] Specifically, first prepare an aqueous solution of acetic acid with a concentration of 0.1M / L, and perform sterile filtration; then mix chitosan with the aqueous solution of acetic acid in a certain ratio, and heat at 45° C. under stirring, Dissolving the chitosan in the aqueous solution of acetic acid to obtain an acetic acid solution of chitosan;

[0084] Then, add the collagen protein solution and water into the acetic acid solution of chitosan in a certain ratio, mix and stir evenly, and obtain the acetic acid solution of collagen-chitosan.

[0085] 2) The obtained collagen-chitosan acetic acid solution was injected into a plate with a diam...

Embodiment 2

[0091] For convenience of description, the three-dimensional scaffold prepared in Example 2 is designated as B.

[0092] The specific preparation method is as follows:

[0093] 1) prepare the acetic acid solution of collagen-chitosan, wherein, the concentration of collagen is 0.1g / L, and the concentration of chitosan is 25g / L;

[0094] Specifically, first prepare an aqueous solution of acetic acid with a concentration of 0.1M / L, and perform sterile filtration; then mix chitosan with the aqueous solution of acetic acid in a certain ratio, and heat at 60° C. under stirring, Dissolving the chitosan in the aqueous solution of acetic acid to obtain an acetic acid solution of chitosan;

[0095] Then, add the collagen protein solution and water into the acetic acid solution of chitosan in a certain ratio, mix and stir evenly, and obtain the acetic acid solution of collagen-chitosan.

[0096] 2) The obtained collagen-chitosan acetic acid solution is injected into 60 central wells of...

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Abstract

The invention relates to the technical field of cell culture and particularly relates to a three-dimensional scaffold applied to cell culture and a preparation method thereof. Through the three-dimensional scaffold, a three-dimensional scaffold structure which meets the production requirement can be supplied to cell culture, the in-vivo culture environment can be simulated and the in-vitro culture effect of the cells can be improved. The three-dimensional scaffold applied to cell culture comprises a porous scaffold material; the content of water in the porous scaffold material is smaller than or equal to 1%; the porous scaffold material comprises collagen and chitosan.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a three-dimensional scaffold for cell culture and a preparation method thereof. Background technique [0002] Cell culture generally refers to all in vitro culture, which means taking out tissues from living animals, separating the tissues into single cells by mechanical methods or enzyme digestion methods, and incubating them under specific in vivo conditions such as simulating the physiological environment in vivo. make it live and grow. [0003] The conventional cell culture mode includes two-dimensional cell culture, which is carried out in cell culture plates such as 2, 4, 6, 12, 24, 48, 96 well cell culture plates or cell culture flasks, bioreactors, etc., cells in the culture medium monolayer adherent growth in a two-dimensional manner. However, multiple studies have found that the gene expression, signal transduction, and morphology of cells cultured in two dimensi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/32A61L15/28A61L15/42A61L27/20A61L27/24A61L27/56
CPCA61L15/325A61L15/28A61L15/425A61L27/20A61L27/24A61L27/56C08L89/00C08L5/08
Inventor 魏搏超孙贺刘庆喜张金花崔雪峰
Owner TIANJIN WEIKAI BIOENG
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