A kind of Acinetobacter ruckeri and its application in degrading pyrethroid pesticides

A technology of Acinetobacter lwudii and cypermethrin, which is applied to the restoration of bacteria and polluted soil, microorganism-based methods, etc., can solve the problems of slow degradation rate and low degradation rate of cypermethrin, achieve rapid degradation, protect the ecological environment, The effect of good application prospects

Active Publication Date: 2020-03-10
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing bacteria have slow degradation rate and low degradation rate to cypermethrin. For example, the patent application with publication number CN101139559A and Xiao Hongli, Separation of Pyrethroid Degrading Strains and Basic Research on Biochemical Molecules, China Excellent Master Thesis Full-text Database Engineering Science and Technology I Series 2005 / 07 discloses a kind of Acinetobacter, when it degrades 200mg / L cypermethrin under medium conditions, the degradation rate in 48h is only 52.1%, and the highest can only reach 75%; Hu Guiping, Cyfluthrin degraded Bacteria FLQ-11-1 isolation and identification, degradation characteristics and degradation mechanism, China Doctoral Dissertation Full-text Database Engineering Science and Technology Series I 2016 No. 08 discloses a bacterium that is resistant to 100mg / L fenpropathrin after 5 days of action The degradation rate is only 90.23%

Method used

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  • A kind of Acinetobacter ruckeri and its application in degrading pyrethroid pesticides
  • A kind of Acinetobacter ruckeri and its application in degrading pyrethroid pesticides
  • A kind of Acinetobacter ruckeri and its application in degrading pyrethroid pesticides

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Embodiment 1

[0027] Embodiment 1 Isolation and identification of Acinetobacter ruckeri of the present invention

[0028] 1. Separation

[0029] Take 4 parts of vinegar sample, mix them well, take 5g each, place in a 250mL Erlenmeyer flask with 30mL LB medium, heat at 80°C for 10min, cool to room temperature and then add cypermethrin to make the mass concentration 50mg / L, cultured at 30°C, 180r / min shaking for 48h. After the end of the culture, the enrichment culture was carried out three times successively according to the inoculum size of 5%, each culture was 48h, and the concentration of cypermethrin in the medium was gradually increased to make it reach 100mg / L, 200mg / L, 400mg / L respectively. Transfer the culture solution after the last level of gradient acclimatization to 30mL LB medium containing 100mg / L cypermethrin according to the inoculum size of 5% (v / v), and use sterile saline as a blank control. min shaking culture 120h.

[0030] Finally, a strain capable of degrading cyper...

Embodiment 2

[0043] Embodiment 2 adopts Acinetobacter ruckerii of the present invention to degrade cypermethrin in soil

[0044] Acinetobacter lwoffii JX-2 of the present invention is taken, and the preservation number is CGMCC No. 13144, referred to as strain JX-2. Strain activation: pick the strain JX-2 into LB liquid medium, 30°C, 180r / min shaking culture for 24h; The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 6 cfu / mL, made into seed solution.

[0045] Add the seed liquid to the soil to be treated, mix well, and that's it.

Embodiment 3

[0046] Embodiment 3 adopts Acinetobacter ruckeri of the present invention to degrade cypermethrin in water

[0047] Acinetobacter lwoffii JX-2 of the present invention is taken, and the preservation number is CGMCC No. 13144, referred to as strain JX-2. Strain activation: pick the strain JX-2 into LB liquid medium, 30°C, 180r / min shaking culture for 24h; The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 7 cfu / mL, made into seed solution.

[0048] Add the seed liquid into the water to be treated, mix well, and you are done.

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Abstract

The invention discloses Acinetobacter ruckeri, which is Acinetobacter lwoffii JX-2 with the preservation number: CGMCC No. 13144 preserved by the General Microorganism Center of China Microbiological Culture Collection Management Committee. Acinetobacter lwoffii JX-2, whose preservation number is CGMCC No.13144, can effectively degrade pyrethroid pesticides, can be used for bioremediation or biopurification of polluted soil and water bodies, protects the ecological environment, and has excellent application prospects .

Description

technical field [0001] The invention relates to a strain of Acinetobacter ruckeri and its application in degrading pyrethroid pesticides. Background technique [0002] Cypermethrin (CY) is one of the most commonly used varieties of pyrethroid pesticides, and it is widely used in the prevention and control of pests in tea trees, fruits and vegetables, humans and animals. Due to its strong hydrophobicity and light and heat stability, its natural degradation rate is slow, and its half-life in the environment is as long as 94.2d-1103d, which easily leads to its residual accumulation in the environment. Therefore, it is widely used in soil, water, agricultural and livestock products. Higher detection rate and exceeding rate often appear in the test. Cypermethrin is highly toxic to fish and other aquatic animals. It can also accumulate in animals and plants, has acute toxicity to the nervous system, and can damage the development of the male reproductive system. Long-term exposur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/306C12N1/205C12R2001/01
Inventor 李建龙代敏孟尧李朝刚付梦茜何圆
Owner CHENGDU MEDICAL COLLEGE
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