A kind of Acinetobacter ruckeri and its application in degrading pyrethroid pesticides
A technology of Acinetobacter lwudii and cypermethrin, which is applied to the restoration of bacteria and polluted soil, microorganism-based methods, etc., can solve the problems of slow degradation rate and low degradation rate of cypermethrin, achieve rapid degradation, protect the ecological environment, The effect of good application prospects
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Embodiment 1
[0027] Embodiment 1 Isolation and identification of Acinetobacter ruckeri of the present invention
[0028] 1. Separation
[0029] Take 4 parts of vinegar sample, mix them well, take 5g each, place in a 250mL Erlenmeyer flask with 30mL LB medium, heat at 80°C for 10min, cool to room temperature and then add cypermethrin to make the mass concentration 50mg / L, cultured at 30°C, 180r / min shaking for 48h. After the end of the culture, the enrichment culture was carried out three times successively according to the inoculum size of 5%, each culture was 48h, and the concentration of cypermethrin in the medium was gradually increased to make it reach 100mg / L, 200mg / L, 400mg / L respectively. Transfer the culture solution after the last level of gradient acclimatization to 30mL LB medium containing 100mg / L cypermethrin according to the inoculum size of 5% (v / v), and use sterile saline as a blank control. min shaking culture 120h.
[0030] Finally, a strain capable of degrading cyper...
Embodiment 2
[0043] Embodiment 2 adopts Acinetobacter ruckerii of the present invention to degrade cypermethrin in soil
[0044] Acinetobacter lwoffii JX-2 of the present invention is taken, and the preservation number is CGMCC No. 13144, referred to as strain JX-2. Strain activation: pick the strain JX-2 into LB liquid medium, 30°C, 180r / min shaking culture for 24h; The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 6 cfu / mL, made into seed solution.
[0045] Add the seed liquid to the soil to be treated, mix well, and that's it.
Embodiment 3
[0046] Embodiment 3 adopts Acinetobacter ruckeri of the present invention to degrade cypermethrin in water
[0047] Acinetobacter lwoffii JX-2 of the present invention is taken, and the preservation number is CGMCC No. 13144, referred to as strain JX-2. Strain activation: pick the strain JX-2 into LB liquid medium, 30°C, 180r / min shaking culture for 24h; The LB slant was cultured at 30°C for 2 days for later use. Wash the bacterial cells on the lower slant with sterile saline and adjust the cell concentration to 10 7 cfu / mL, made into seed solution.
[0048] Add the seed liquid into the water to be treated, mix well, and you are done.
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