Complete-set reagent and method for identifying syngnathus

A sea dragon and reagent technology, applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., can solve problems such as the confusion of sea dragon medicinal materials

Active Publication Date: 2017-05-31
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although sea dragon is rich in resources, it is also a precious marine drug resource, and the price of different specifications of sea dragon is also different. For a long time, counterfeit sea dragon with low price has been often used to pretend to be genuine sea dragon in the Pharmacopoeia, which has led to confusion in the medicinal material market.

Method used

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  • Complete-set reagent and method for identifying syngnathus
  • Complete-set reagent and method for identifying syngnathus
  • Complete-set reagent and method for identifying syngnathus

Examples

Experimental program
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Effect test

Embodiment 1

[0090] Embodiment 1, the preparation of the complete set of reagents for identifying or assisting in the identification of Sea Dragon

[0091] The present invention provides a complete set of reagents for identification or auxiliary identification of sea dragon, consisting of primer pairs named HLN-P, HLD-P and HLJ-P respectively;

[0092] HLN-P is composed of single-stranded DNA named HLN-S and HLN-A respectively; HLN-S is the single-stranded DNA shown in sequence 1 in the sequence listing, and HLN-A is the single-stranded DNA shown in sequence 2 in the sequence listing DNA;

[0093] HLD-P consists of single-stranded DNA named HLD-S and HLD-A respectively; HLD-S is the single-stranded DNA shown in sequence 3 in the sequence listing; HLD-A is the single-stranded DNA shown in sequence 4 in the sequence listing DNA;

[0094] HLJ-P is composed of single-stranded DNA named HLJ-S and HLJ-A respectively; HLJ-S is the single-stranded DNA shown in sequence 5 in the sequence listing;...

Embodiment 2

[0096] Embodiment 2. Using the identification of Embodiment 1 or the complete set of reagents to assist in the identification of sea dragons to identify sea dragons

[0097] 1. Samples

[0098] 24 sea dragon samples were selected, including 3 authentic products and 3 counterfeit products, 3 genuine products: 5 pseudo sea dragons, 4 diao sea dragons, 6 sharp sea dragons; 3 fake sea dragons: 3 Baojia sea dragons, 5 crude kiss sea dragons and 1 Thorny Sea Dragon. These 24 sea dragon samples were identified by researcher Li Junde, and the certificated specimens are preserved in the Chinese Medicine Resource Center of the China Academy of Chinese Medical Sciences. The experimental materials are shown in Table 1.

[0099] Table 1. Experimental materials

[0100]

[0101] Extract the genomic DNA of the above samples respectively, and then use primers to carry out PCR amplification of SH-P to obtain the SH-P PCR products of each sample respectively. The names of SH-P are respecti...

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Abstract

The invention discloses a complete-set reagent and method for identifying syngnathus. The complete-set reagent disclosed by the invention for identifying syngnathus is prepared from primer pairs of HLN-P, HLD-P and HLJ-P, wherein the HLN-P is formed by single stranded DNA shown in a sequence 1 and a sequence 2 in a sequence table, the HLD-P is formed by single stranded DNA shown in a sequence 3 and a sequence 4 in the sequence table, the HLJ-P is formed by single stranded DNA shown in a sequence 5 and a sequence 6 in the sequence table, and SH-P is formed by single stranded DNA shown in a sequence 7 and a sequence 8 in the sequence table. Experiments show that the complete-set reagent and method disclosed by the invention for identifying or assistantly identifying can successfully identify salable syngnathus from fake syngnathus and also can distinguish syngnathoides, solegnathus and syngnathus acus from salable syngnathus. The complete-set reagent disclosed by the invention for identifying or assistantly identifying syngnathus has high specificity and good stability for identifying syngnathus; in addition, the accuracy rate is 100%.

Description

technical field [0001] The invention relates to a set of reagents and a method for identifying sea dragons in the field of biotechnology. Background technique [0002] The traditional Chinese medicine sea dragon is the dried body of the Sea dragon family animal Diao sea dragon Solenognathus hardwickii (Gray), pseudo sea dragon Syngnathoides biaculeatus (Bloch) or sharp sea dragon Syngnathus acus Linnaeus. It has the effects of warming the kidney and strengthening yang, dispelling stagnation and reducing swelling. It is mainly used for kidney-yang deficiency, impotence and nocturnal emission, accumulation of lumps in the abdomen, scrofula with phlegm nucleus, injury from falls, and external treatment of carbuncle and furuncle. Modern pharmacological experiments have proved that it has anti-fatigue, anti-tumor and sex hormone-like effects. [0003] In Qing Dynasty Zhao Xuemin's "Compendium of Materia Medica Supplements", "Chi Chi Ji Ji" records: "Sea dragon is produced in Pen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2565/125
Inventor 黄璐琦袁媛张夏楠赵玉洋蒋超
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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