An ultrafast NMR two-dimensional j-spectroscopy method against inhomogeneous magnetic fields

Abstract

The present invention provides a method for ultra-fast acquisition of nuclear magnetic resonance two-dimensional J-decomposition spectrum in a single scan against a single-direction inhomogeneous magnetic field environment, and uses the joint action of the selective excitation module and the refocusing sampling module to break through the limitation of the traditional two-dimensional J-spectrum method Limitations: Obtaining a two-dimensional J-spectrum with ultra-fast sampling in a single-direction inhomogeneous magnetic field environment effectively eliminates the influence of a single-directional magnetic field inhomogeneity, greatly shortens the experimental time, and expands the application field of the two-dimensional J-spectrum. At the same time, this method is suitable for conventional nuclear magnetic resonance spectrometers, does not require any special hardware devices, and does not require any special sample pretreatment process. .

Description

technical field [0001] The invention relates to nuclear magnetic resonance (Nuclear Magnetic Resonance, NMR) spectroscopy molecular structure information detection technology, in particular to a single-scan ultra-fast acquisition of high-resolution two-dimensional J-spectrum in a single-direction non-uniform magnetic field environment. Background technique [0002] Due to the unique advantages of non-destructive and non-invasive detection of NMR spectroscopy, NMR technology has been widely used in biology, chemistry, physics, medicine, materials science and other disciplines in recent decades. In 1971, scholar Jeener proposed the concept of two-dimensional spectrum on the basis of one-dimensional spectrum, extending NMR from one-dimensional spectrum to two-dimensional spectrum. In conventional nuclear magnetic resonance spectroscopy detection, two-dimensional spectroscopy overcomes the problems of spectral crowding and peak assignment difficulties that often exist in one-dim...

AI Technical Summary

Problems solved by technology

Although the traditional two-dimensional J spectrum method provides a general method for obtaining nuclear chemical shift and coupling information, in practical applications, this technique has some limitations.

First of all, the traditional two-dimensional J spectrum method requires two-dimensional sampling, which takes a long time under the premise of ensuring the resolution of the spectrum

This problem limits the application of this method in real-time monitoring of chemical reactions and other fields, and also limits the development and application of this method to multidimensional spectroscopy.

Secondly, the acquisition of the traditional two-dimensional J-spectrum is limited by the uniformity of the magnetic field, and an ideal two-dimensional J-spectrum needs to be obtained under a relatively uniform magnetic field.

In practical applications, for homogeneous solution sample systems, it is necessary to perform complex and time-consuming shimming operations on the detection samples to obtain an ideal magnetic field environment; while for heterogeneous samples such as viscous samples and biological tissue samples , the magnetic susceptibility of the sample itself will bring large inhomogeneity of the magnetic field, and it is often difficult or even impossible to obtain a sufficiently high magnetic field uniformity

This problem also limits the scope of application of the traditional J-spectrum method

Images