A Pure Chemical Shift NMR Spectroscopy Method Resistant to Magnetic Field Inhomogeneity

Abstract

The invention provides a pure chemical shift nuclear magnetic resonance spectrum method capable of resisting non-uniform interference of a magnetic field and relates to nuclear magnetic resonance spectroscopy detection methods. The method comprises the following steps: mounting a to-be-detected sample into a standard nuclear magnetic test tube, and transferring the standard nuclear magnetic test tube to a nuclear magnetic spectrograph detection cavity; by invoking a conventional one-dimensional hydrogen spectrum sequence, acquiring a one-dimensional hydrogen spectrum of the to-be-detected sample, so as to obtain signal spectrum peak distribution range and line width information of the to-be-detected sample; measuring the pulse width of a non-selectivity 90-degree radio-frequency pulse and the pulse width and powder of a solvent selectivity (90 degrees) I radio-frequency pulse required for stimulating the to-be-detected sample; importing a nuclear magnetic resonance pulse sequence in a nuclear magnetic resonance spectrometer, and starting a signal stimulation module, an indirect constant-dimension evolution module, an intermolecular zero-quantum coherent signal selection module and a signal sampling module of the pulse sequence; setting experiment parameters of each module of the pulse sequence in an experiment, and carrying out data sampling; and after data acquisition, carrying out data post-processing including spectrogram folding correction and signal linear prediction by invoking a data post-processing codes, so as to obtain a high-resolution pure chemical shift spectrum free from the non-uniform interference of the magnetic field.

Description

technical field [0001] The invention relates to a nuclear magnetic resonance (NMR, Nuclear Magnetic Resonance) spectroscopy detection method, in particular to an anti-magnetic field inhomogeneity-resistant magnetic field inhomogeneous and high-resolution pure chemical shift spectrum obtained in any magnetic field environment, especially in an inhomogeneous magnetic field environment. Uniform pure chemical shift NMR spectroscopy method. Background technique [0002] As a widely used non-destructive testing and analysis technique, nuclear magnetic resonance spectroscopy can provide chemical information at the molecular level, and is often used for component identification and structural analysis of chemical and biological samples. In nuclear magnetic resonance spectroscopy detection, due to the high sampling efficiency of one-dimensional hydrogen spectrum, and in the natural abundance, hydrogen nuclei have higher signal sensitivity than other nuclei that can be used for nuclea...

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Problems solved by technology

[0004] However, the above pure chemical shift spectroscopy methods can only be applied to the homogeneous solution sample system with a uniform external magnetic field environment. Therefore, before using these methods to perform pure chemical shift experiments, it is necessary to perform cumbersome shimming operations on the samples to be tested.

For heterogeneous samples such as viscous samples and biological tissue samples, the magnetic field inhomogeneity caused by its own magnetic susceptibility is difficult to eliminate through shimming operations, which makes the application of the above pure chemical shift spectroscopy method to such samples more difficult

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