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Method for research on influence of temperature on embryonic development and growth of sea cucumber

A technique for embryo development and sea cucumber, applied in the research field of the effect of temperature on the embryonic development and growth of sea cucumber, can solve the problems of change and complexity, and achieve the effect of reasonable design and ingenious temperature gradient setting.

Inactive Publication Date: 2017-07-04
QINGDAO QINGQUAN BIOTECH CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

Since the japonicus enters the wild environment from the early planktonic larval stage, it is more complex and variable compared with indoor cultivation conditions.

Method used

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Embodiment Construction

[0007] The present invention will be described in further detail below in conjunction with specific embodiments.

[0008] A research method for the influence of temperature on the development and growth of japonicus japonicus embryos, the experimental design is as follows:

[0009] The brood references with mature gonads released sperm and eggs after being stimulated by heating, and samples were taken for observation after 4 hours. Fertilized eggs with a fertilization rate > 99% and a diameter greater than 160 μm were selected for the experiment. At the beginning of the experiment, the fertilized eggs had been hatched in the water body at 22.5°C for about 6–7 hours, and they were in the early blastocyst stage. The blastocysts were put into the pre-prepared seawater with various temperature gradients. The seawater used was natural seawater filtered by 800 filter bags after sand filtration, with a salinity of ~31 and a pH of 7.8~8.2. Each temperature gradient was set as follows...

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Abstract

The invention relates to a method for research on influence of a temperature on embryonic development and growth of sea cucumber. The method comprises the following steps: allowing sea cucumber parents with maturely-developed gonad to release sperms and ova through heating stimulation, then after 4 h, carrying out sampling and observing, and selecting fertilized ova with a fertilization rate larger than 99% and a diameter larger than 160 [mu]m for an experiment; before the experiment begins, incubating the fertilized ova in water at 22.5 DEG C for 6 to 7 h so as to obtain blastocysts in an early period; respectively placing the blastocysts into prepared seawater with temperature gradients, a salinity of 31 and a pH value of 7.8 to 8.2, wherein the temperature gradients are arranged as follows: 5, 10, 12, 15, 19, 21, 25, 28, 31 and 34 DEG C; carrying out indoor natural lighting by day and no lighting at night; allowing the culturing density of each group to be 0.5 ind / ml, and replacing half of water once every two days, wherein air is not inflated, and oceanic red yeast is used as a bait when embryos are developed to larvae; and sampling 15 to 30 larvae every two days, and carrying out observation and measurement. The method provided by the invention is reasonable in design, has ingenious temperature gradient arrangement, and can conveniently and effectively determine the influence of temperature change on embryonic development and growth of the sea cucumber.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a research method for the influence of temperature on the development and growth of sea cucumber embryos. Background technique [0002] Sea cucumber ecological seed breeding is a method based on ecological principles, making full use of natural environmental conditions, adopting high-efficiency facilities and ecological breeding mode, and producing high-quality and healthy seedlings through scientific management. Since sea cucumber enters the wild environment from the early stage of planktonic larvae, it is more complex and changeable compared with indoor cultivation conditions. Affected by the alternation of day and night, tide, precipitation and other environmental factors, the changes of temperature, salinity, turbidity and plankton in the water body are more severe, which will inevitably affect the development and growth of A. japonicus larvae. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/30
CPCA01K61/00
Inventor 褚方强
Owner QINGDAO QINGQUAN BIOTECH CO LTD
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