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Method for improving strip virus resistance of rice by using melatonin

A rice stripe virus and melatonin technology, applied in the field of agricultural science, can solve problems such as time-consuming, environmental pollution, and difficult to achieve results

Inactive Publication Date: 2017-09-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the selection and breeding of resistant varieties has a large workload and takes a long time, and it is difficult to achieve good results in the short term. Although chemical agents can block the spread of the virus, their efficiency is not high and may cause environmental pollution

Method used

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  • Method for improving strip virus resistance of rice by using melatonin
  • Method for improving strip virus resistance of rice by using melatonin
  • Method for improving strip virus resistance of rice by using melatonin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1, utilize melatonin medicament to pretreat the method for rice investigation its state of an illness

[0012] 1. Plant material, virus analysis and infection method

[0013] Nihonbare (Oryza sativa L.cv.Nipponbare) seeds were first treated with 0.1% HgCl 2 Disinfect for 1 hour; then rinse with deionized water, soak the seeds at 25°C for 2 days, and germinate for 1 day; the seeds after germination are sown in a 1L beaker for cultivation, 40 seeds per cup, watered with an appropriate amount of Hoagland nutrient solution every two days, light for 14 hours, and dark 10h, temperature 28±1℃.

[0014] Carrying RSV and non-toxic SBPH were kept in the pest room of the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. After the first instar larvae hatched, they were separately raised on healthy Oryza sativa L.cv.Wuyujing NO.3 rice seedlings, placed in an insect culture room at 25°C, and the third to fourth instar L. striatellus It can be used ...

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Abstract

The invention provides a method for reducing the incidence of the stripe virus disease of rice by using melatonin (MT). The rice is treated with the melatonin with the concentration of 0.1-100 [mu]M 12 hours before inoculation. The method can be used to improve the rice strip virus resistance of rice, and can be applied o the fields of agriculture production, scientific research and environmental protection in order to realize the economical and environmentally-friendly reduction of disease caused grain loss.

Description

Technical field: [0001] The invention uses melatonin (Melatonin, MT) to pretreat rice, can improve the resistance of rice to rice stripe virus (Rice stripe virus, RSV), can be applied in agricultural production, and belongs to the field of agricultural science and technology. Background technique: [0002] RSV belongs to the genus Tenuivirus, and its transmission medium is mainly Laodelphax striatellus (SBPH). The shape of the virus particle is a branched filament, and the genome consists of four RNAs, named RNA1, RNA2, RNA3, and RNA4, all of which can encode proteins for virus response synthesis or transfer-related functions. Among them, the nucleoprotein protein (NCP) encoded by RNA3 and the virus-specific protein (Specificity protein, SP) encoded by RNA4 play a key role in the pathogenic process of the virus. At the early stage of the disease, short yellow-white stripes will appear at the base of the heart leaf, and then slowly merge, and further become yellow-green stri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/38A01P21/00
CPCA01N43/38
Inventor 周彤周益军卢荣飞杜琳琳兰莹孙枫
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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