A kind of extraction method of Dendrobium officinale leaf and its extract and application

A technology of Dendrobium officinale leaf extract, which is applied in the field of extraction of Dendrobium officinale leaves, can solve the problems of extraction yield and purity of functional components, failure to improve the utilization efficiency of Dendrobium officinale leaves, and low utilization efficiency, so as to achieve firmer skin It is also elastic, improves acne, improves pigmentation, and improves anti-aging and anti-oxidation effects

Active Publication Date: 2020-09-18
连南瑶族自治县欣胜堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the unextracted leaves of Dendrobium candidum are difficult to be absorbed by the human body, so it is difficult for the leaves of Dendrobium candidum to be widely used. Most people have very limited awareness of the efficacy of Dendrobium candidum leaves. Often discarded or used as feed, the utilization efficiency is extremely low
[0005] For this reason, there is an urgent need for an extraction method of Dendrobium candidum leaves, but the existing extraction methods of Dendrobium candidum leaves are basically simple alcohol precipitation methods, such as the invention patent of 201610155974.6, which cannot improve the utilization efficiency of Dendrobium candidum leaves. There are certain restrictions on the extraction yield and the purity of functional components

Method used

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  • A kind of extraction method of Dendrobium officinale leaf and its extract and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for extracting dendrobium officinale leaves, comprising the steps of:

[0052] (1) Slurry: take the Dendrobium candidum leaf slurry, the Dendrobium candidum leaf slurry is composed of Dendrobium candidum leaf powder and water, wherein, the parts by weight of the Dendrobium candidium leaf powder and water in the Dendrobium candidium leaf slurry are 20 parts and 75 parts respectively share;

[0053] (2) Repeated freezing and thawing: the dendrobium candidum leaf slurry was cooled to -10°C, kept for 24 hours, then heated to 100°C, kept for 2 hours, then cooled to -10°C, kept for 24 hours, and then heated to a slurry state;

[0054] (3) Enzymolysis: adding protease to the Dendrobium officinale leaf slurry after repeated freezing and thawing in the step (2) for enzymolysis reaction, wherein the protease is papain, and the amount of the protease is 2.5 parts by weight. The temperature is 45°C, and the enzymatic hydrolysis time is 4h;

[0055] (4) Inactivation: heat...

Embodiment 2

[0058] The difference between this embodiment and embodiment 1 is:

[0059] A method for extracting dendrobium officinale leaves, comprising the steps of:

[0060] (1) Slurry: Take the Dendrobium candidum leaf slurry, the Dendrobium candidum leaf slurry is composed of Dendrobium candidum leaf powder and water, wherein, the parts by weight of the Dendrobium candidium leaf powder and water in the Dendrobium candidium leaf slurry are 1 part and 50 parts respectively share;

[0061] (2) Repeated freezing and thawing: cooling the Dendrobium officinale leaf slurry to -20°C, keeping it warm for 12 hours, then heating to 80°C, keeping it warm for 1 hour, then cooling to -20°C, keeping it warm for 12 hours, and then heating up to a slurry state;

[0062] (3) Enzymolysis: adding protease to the Dendrobium candidum leaf slurry after repeated freezing and thawing in the step (2) for enzymolysis reaction, wherein the protease is bromelain, and the amount of the protease is 0.1 parts by we...

Embodiment 3

[0066] The difference between this embodiment and embodiment 1 is:

[0067] A method for extracting dendrobium officinale leaves, comprising the steps of:

[0068] (1) Slurry: Take the Dendrobium candidum leaf slurry, the Dendrobium candidum leaf slurry is composed of Dendrobium candidum leaf powder and water, wherein the weight parts of Dendrobium candidium leaf powder and water in the Dendrobium candidium leaf slurry are 40 parts and 98 parts respectively share;

[0069] (2) Repeated freezing and thawing: cooling the Dendrobium candidum leaf slurry to 0°C, keeping it warm for 36 hours, then heating it to 90°C, keeping it warm for 3 hours, then cooling it to 0°C, keeping it warm for 36 hours, and then heating it up to a slurry state;

[0070] (3) Enzymolysis: adding protease to the Dendrobium officinale leaf slurry after repeated freezing and thawing in the step (2) for enzymolysis reaction, wherein the protease is composed of papain and bromelain in a weight ratio of 1:1 C...

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Abstract

The invention relates to the technical field of dendrobium officinale, and particularly relates to an extraction method of dendrobium officinale leave, an extract of the dendrobium officinale leave and application of the extract of the dendrobium officinale leave. The extraction method jointly uses a repeated freezing and thawing wall breaking technology and an enzymolysis technology, so that the extraction rate of the dendrobium officinale leave is 60% or more, the purity of effective components such as protein, polysaccharide and total flavone reaches 90%, the obtained extract of the dendrobium officinale leave also contains rich antioxidant components such as anthocyanidin, naringenin, lignan, dihydroisoflavone and the like, indicating that the extract of the dendrobium officinale leave has a bright application prospect in the field of skin care products.

Description

technical field [0001] The invention relates to the technical field of dendrobium candidum, in particular to a method for extracting leaves of dendrobium candidum and its extract and application. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of Orchidaceae. Sweet in the mouth, slightly cold, Guizhou, Kidney Channel. Ancient Chinese medicine classics "Shen Nong's Materia Medica" and "Compendium of Materia Medica Supplements" have recorded that fresh or dried stems are used as medicines, which have the effects of nourishing yin and clearing heat, nourishing stomach and promoting body fluid, tonifying kidney and replenishing essence. [0003] However, in the prior art, only the stem is used as a valuable medical material, and other parts of Dendrobium candidum, such as Dendrobium candidum leaves and Dendrobium candidum flowers, are rarely used. According to the actual detection, the leaves of Dendrobium candidum contain more ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8984A61P39/06A61K8/9794A61Q19/02A61Q19/08A61K127/00
CPCA61K8/97A61K36/8984A61K2236/10A61K2236/19A61K2800/805A61Q19/02A61Q19/08
Inventor 张春艳何伟彬范钰屏
Owner 连南瑶族自治县欣胜堂生物科技有限公司
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