An efficient sterile sowing and seedling raising method for hybrid seeds of camellia nitidissima Chi
A hybrid seed and aseptic sowing technology, applied in the field of plant seedling raising, can solve the problems of hybrid germplasm loss and low seedling emergence rate of golden camellia tea, and achieve the effects of shortening the seeding and seedling time, promoting the germination of many seedlings, and improving the seedling raising and sowing efficiency.
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Embodiment 1
[0032] An efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds, comprising the following steps:
[0033] Step 1: Select hybrid seeds with plump seeds, no diseases and insect pests and no trauma (seeds of the hybrid species of Camellia japonica♀ and Camellia ‘Liexiang’♂);
[0034] Step 2: Disinfect the surface of the hybrid seeds in step 1 with alcohol with a volume concentration of 75% for 50 seconds, then rinse twice with sterile water, then disinfect with a sodium hypochlorite solution with a mass concentration of 2.2% for 4 minutes, and finally rinse with sterile water Rinse more than 5 times to obtain sterile hybrid seeds, wherein the time for each rinse with sterile water is 2 minutes;
[0035] Step 3: Pack the washed and drained fine sand into culture bottles. The thickness of each bottle of fine sand is about 2cm. Seal the culture bottle and place it at a temperature of 121°C and a pressure of 1.1kg / cm 2 Sterilize under the con...
Embodiment 2
[0040] An efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds, comprising the following steps:
[0041] Step 1: Select hybrid seeds with plump seeds, no pests and injuries (seeds of the hybrid species of Camellia japonica♀ and Camellia japonica♂ in Fangcheng);
[0042] Step 2: Disinfect the surface of the hybrid seeds in step 1 with alcohol with a volume concentration of 75% for 70 seconds, then rinse with sterile water for 4 times, then disinfect with a sodium hypochlorite solution with a mass concentration of 2.6% for 5 minutes, and finally rinse with sterile water Rinse more than 5 times to obtain sterile hybrid seeds, wherein the time for each rinse with sterile water is 2 minutes;
[0043] Step 3: Pack the washed and drained fine sand into culture bottles. The thickness of each bottle of fine sand is about 2cm. Seal the culture bottle and place it at a temperature of 121°C and a pressure of 1.1kg / cm 2 Sterilize under the conditi...
Embodiment 3
[0048] An efficient method for aseptic sowing and seedling cultivation of Camellia japonica hybrid seeds, comprising the following steps:
[0049] Step 1: Select hybrid seeds with plump seeds, no pests and injuries (seeds of hybrids of Fangcheng Camellia ♀ and Vietnamese Muscle Tea ♂);
[0050] Step 2: Disinfect the surface of the hybrid seeds in step 1 with alcohol with a volume concentration of 75% for 60 seconds, then rinse with sterile water for 3 times, then disinfect with a sodium hypochlorite solution with a mass concentration of 2.4% for 5 minutes, and finally rinse with sterile water Rinse more than 5 times to obtain sterile hybrid seeds, wherein the time for each rinse with sterile water is 2 minutes;
[0051] Step 3: Pack the washed and drained fine sand into culture bottles, the thickness of each bottle of fine sand is about 2cm, seal the culture bottle, and then place it at a temperature of 121°C and a pressure of 1.1kg / cm 2 Sterilize under the condition of 30min...
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