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Composition for improving circadian rhythm

A circadian rhythm and composition technology, which can be used in drug combinations, active ingredients of heterocyclic compounds, nervous system diseases, etc., can solve problems such as the safety of caffeine, improve circadian rhythm, alleviate or treat circadian rhythm disorders, improve effect of falling asleep

Active Publication Date: 2017-11-28
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a problem of safety when caffeine is taken in excess

Method used

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  • Composition for improving circadian rhythm
  • Composition for improving circadian rhythm
  • Composition for improving circadian rhythm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065]

[0066]As the cell line, a fetal fibroblast cell line derived from Per2::LUC knock-in mice was used. The Per2::LUC knock-in mouse is a mouse in which a luciferase gene as a reporter gene has been introduced downstream of the promoter region of the biological clock gene Per 2. Thus, in fetal fibroblast cell lines derived from Per 2::LUC knock-in mice, luciferase gene expression was accompanied by periodic expression of circadian clock genes. When fetal fibroblasts derived from Per 2::LUC knock-in mice were cultured in a luciferin-containing medium as a luciferase substrate, since the cells periodically chemiluminescent, the circadian clock gene Per was evaluated by monitoring its luminescence. 2 expression rhythm.

[0067] First, by inoculating fetal fibroblast cell lines from Per2::LUC knock-in mice about 5×10 5 After being seeded in a 35mm culture dish, they were treated with 200nM dexamethasone for 2 hours, so that the biological rhythm of the cells was temporari...

Embodiment 2

[0070]

[0071] As animals, the Per 2::LUC knock-in mice described in Example 1 were used. Since the luciferase gene, a reporter gene downstream of the circadian clock gene, is periodically expressed in this mouse, chemiluminescence occurs when luciferin, a luminescent substrate, is administered from the outside. Therefore, the expression of the biological clock gene Per 2 in the body can be monitored over time.

[0072] First, Per 2::LUC knock-in mice were forcibly orally administered a mixture of the test substance sesamin / episesamin=1 / 1 in an amount of 250 mg / kg body weight per day for 3 days. As a solvent control, olive oil was used. Three hours after the last administration, a 15 mg / kg fluorescein solution was subcutaneously administered to the back of the mouse under isoflurane inhalation anesthesia. The subsequent chemiluminescence was detected by Invivo imaging (IVIS) kinetics system (Caliper Life Sciences). The detection of chemiluminescence was carried out for t...

Embodiment 3

[0075]

[0076] The test method was performed in the same manner as in Example 1 above. As test substances, 10 μM of sesamin, episesamin, and sesaminol were added and their effects were evaluated. The result is as Figure 4 shown. As a result, it was confirmed that any of sesamin, episesamin, and sesaminol can increase the amplitude of Per2 gene expression.

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Abstract

The purpose of the present invention is to provide a composition for improving circadian rhythm that can be safely taken over a long time, in particular, a composition for controlling circadian rhythm that is capable of increasing circadian rhythm amplitude. Provided is a composition for improving circadian rhythm that contains one or more kinds of lignan compounds.

Description

technical field [0001] The present invention relates to a composition for improving circadian rhythm. Specifically, it relates to a composition for improving circadian rhythm by including a lignan compound and increasing the amplitude of periodic expression levels of biological clock genes. Background technique [0002] Many biological activities and physiological phenomena show automatic periodic fluctuations called biological rhythms, and among them, a rhythm showing a periodicity of about 24 hours is called a circadian rhythm (circadian rhythm). Among the biological activities and physiological phenomena having a circadian rhythm are sleep and waking, fluctuations in blood pressure, fluctuations in body temperature, eating behaviors, and the like. [0003] All cells constituting the body each have a mechanism for emitting a circadian rhythm. This circadian rhythm generation mechanism changes the expression level in a 24-hour cycle based on a plurality of gene groups cal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/36A61P25/20A61P43/00
CPCA61K31/36A61P25/00A61P25/20A61P43/00
Inventor 立石法史柴田重信
Owner SUNTORY HLDG LTD
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