Seed selection method for cold resistant and bolting resistant brassica oleracea var

Abstract

The invention discloses a seed selection method for cold resistant and bolting resistant brassica oleracea var. According to the invention, original cabbage excellent self-incompatible line and (male parent) and other cabbages (fertile, female parent) are subjected to hybridization through a castration florescence, progeny is subjected to backcrossing fourth generation through the emasculation florescence, fine spraying is carried out on stigma by warm water with sodium bicarbonate having concentration being 1% at the temperature of 28-32 DEG C at florescence, the substances of the stigma such as callose is broken up, so that self-incompatibility is changed to self-compatibility, and the free pollination by honeybees among the plants at the florescence finally. On one hand, the original parent enables purification and renovation, on the other hand, through multi-generation florescence pollination, the self-incompatibility is reduced, the self-compatibility is enhanced, self-incompatibility is gradually changed to self-compatibility, for the utilization of breeding of a cytoplasmic male sterile line, the breeding efficiency is increased, the disadvantages of self incompatibility and artificial bud pollination required by line breeding can be overcome, at the same time, for the phenomena of multi-year inbred parent property degeneration, such as strong plant growth vigor, less bud abortion rate, large pollen amount, and high setting rate, the method finds a novel method for purification and renovation.

Description

technical field [0001] The invention relates to a breeding method for cold-resistant and bolting-resistant head cabbage, which belongs to the technical field of head cabbage breeding. Background technique [0002] Heading cabbage (Brassica oleacea L.var.cap itata L.) is a variety of Brassica plants in the cruciferous family. It originated from the Mediterranean coast and was introduced to China in the 16th century. Heading cabbage has the characteristics of disease resistance, strong adaptability, easy storage and transportation, high yield, and good quality. Because of its wide adaptability, strong stress resistance, stable yield, and storage and transportation resistance, its cultivation has developed rapidly in my country. At present, the annual planting area has reached 3 million hectares 2 All of the above plays an important role in the annual vegetable supply and export trade. [0003] Since the early 1980s, my country began to introduce some foreign varieties of cabb...

AI Technical Summary

Problems solved by technology

The previously accumulated good self-incompatibility line parent materials are facing elimination or upgrading. For example, long-term continuous self-trading will cause viability degradation, and some excellent traits will be lost, such as weak plant growth, high bud failure rate, and less pollen. The hybridization rate of hybrids is difficult to reach 100%, especially the self-incompatible lines need to be artificially pollinated at the bud stage, the workload is heavy, and the parents are difficult to reproduce.

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