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Efficient subculture and rapid propagation method for ceratostigma willmottianum

A snowflake, high-efficiency technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as the lack of in-depth research on plants, and achieve the effects of large reproduction coefficient, reduced production costs, and rapid growth.

Active Publication Date: 2018-02-09
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there are relatively few studies on the tissue culture technology of Minjiang blue snowflake, which may be related to the in-depth research on the plant

Method used

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  • Efficient subculture and rapid propagation method for ceratostigma willmottianum
  • Efficient subculture and rapid propagation method for ceratostigma willmottianum
  • Efficient subculture and rapid propagation method for ceratostigma willmottianum

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Embodiment 1

[0031] In vitro sterilization and disinfection of explants: 400 times of carbendazim was used to spray and water Minjiang blue snowflake seedling potted plants once a week (four times in total). From 9 to 10 am on a sunny day, select the upper part of the young and tender stems with buds from the branches of the mother plant of Minjiang Blue Snowflake, which is healthy and free from diseases and insect pests. Leave a little petiole and remove the leaves and cut them into 3-5cm small sections. After gently brushing off the excess impurities and dirt on the surface with a brush, soak it with detergent for 30 minutes, then gently brush off the softened stubborn impurities on the surface with a brush, and finally rinse with mild running water for 2 to 3 hours. Drain. After completing the above steps, use 75% ethanol on the ultra-clean workbench for 30 seconds, and then rinse with sterile water for 3 to 4 times. disinfection. Rinse gently with plenty of sterile water 5-6 times be...

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Abstract

The invention provides an efficient subculture and rapid propagation method for ceratostigma willmottianum. The efficient subculture and rapid propagation method includes steps of shearing aseptic seedlings of ceratostigma willmottianum offspring to obtain explants, placing the explants in culture media without exogenous hormones and carrying out expanding propagation and proliferation culture onthe explants. The aseptic seedlings of the ceratostigma willmottianum offspring are obtained by means of preliminary tissue culture and breeding; each stem of each explant is provided with a leaf. Theculture media without the exogenous hormones are MS culture media with added sucrose and added agar. A preliminary tissue culture and breeding process includes acquiring tender stems of the upper half portions of ceratostigma willmottianum branches and utilizing the tender stems with buds as explants; sequentially carrying out auxiliary bud induction culture, auxiliary bud proliferation culture and rooting culture. The efficient subculture and rapid propagation method has the advantages that the breeding production cycle of the ceratostigma willmottianum can be shortened, and the production cost can be reduced; the efficient subculture and rapid propagation method has a large propagation coefficient and can be easily and feasibly implemented, the ceratostigma willmottianum can quickly grow, and materials can be acquired at any time, so that aseptic rapid propagation can be carried out.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to the Minjiang blue snowflake rapid propagation technology, in particular to a Minjiang blue snowflake rapid propagation method with high efficiency. Background technique [0002] Minjiang blue snowflake (Ceratostigma willomottianum Stapf) is a species endemic to China. It is a deciduous semi-shrub belonging to the genus Ceratostigma willomottianum Stapf, also known as Zijinlian. As a new type of garden ornamental medicinal plant with both ornamental and medicinal value, it is popular because of its light and elegant purple flowers, scattered branches, and clusters of flowers on the inflorescence, or it can be used as a potted plant for people to watch. Or widely used as garden greening seedlings. In addition, it contains a variety of phytochemical active ingredients, which are traditional ethnic medicines and have been widely used for a long time. In addition, plumbag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡菊高素萍洪梅婷陈曦雷霆
Owner SICHUAN AGRI UNIV
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