Tobacco explant browning prevention method based on activated carbon

An explant and activated carbon technology, applied in the field of plant tissue culture, can solve the problems of death and reduce the viability of explants, and achieve the effects of accelerated growth, low cost and improved differentiation state.

Active Publication Date: 2018-03-30
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term low-light cultivation will reduce the viability of explants and even cause death

Method used

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  • Tobacco explant browning prevention method based on activated carbon
  • Tobacco explant browning prevention method based on activated carbon
  • Tobacco explant browning prevention method based on activated carbon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The tobacco explant anti-browning method based on activated carbon of the present embodiment may further comprise the steps:

[0028] Step (1), disinfection of seeds: take plump tobacco seeds, then move them to an ultra-clean workbench, soak the seeds with 75% alcohol for 30s, rinse them twice with sterile water, and then use 1% AgNO 3 Disinfect for 10 minutes, soak and wash in sterile water for 5 times, dry the surface moisture of the explants with sterile filter paper, and then inoculate, wherein the sterile water is ultrapure water sterilized by high pressure.

[0029] Step (2), seed cultivation: inoculate the seeds obtained in step (1) into MS medium, cultivate at a temperature of 25±1°C, and light intensity of 30 μmol / (m 2 s), light time is 16h / d under the condition of cultivating 60d, seed germination grows into seedling, and described seed MS culture medium is the agar that adds 30g / L sucrose and 4g / L in MS basic culture medium, the pH of culture medium The valu...

Embodiment 2

[0038] The tobacco explant anti-browning method based on gac of the present embodiment, wherein:

[0039] Steps (1)-(3) are the same as in Example 1;

[0040] Step (4), anti-browning culture: place the explants obtained in step (3) in MS anti-browning medium, and culture them under light at 28°C for 16h / d, and the light intensity is 30-50μmol / (m 2s), cultivated under the condition of dark cultivation at 25°C for 8h / d for 40d respectively, and the MS anti-browning medium was MS basal medium, adding 2.5g / L activated carbon, 30g / L sucrose, and 4g / L agar, and cultured The pH of the base is 5.7.

[0041] Step (5), rooting culture: put the explants obtained in step (4) in MS rooting medium, and cultivate them under light at 28°C for 16h / d, with light intensity of 45μmol / (m 2 s), cultivated under the condition of 25 DEG C of dark cultivation for 8h / d, and the tobacco took root when cultivating for 5d, and the MS rooting medium was the agar that added 2.5g / L gac, 30g / L sucrose and 4...

Embodiment 3

[0045] The tobacco explant anti-browning method based on activated carbon of the present embodiment may further comprise the steps:

[0046] In steps (2)-(3), the light intensity is 50 μmol / (m 2 s);

[0047] Step (4), anti-browning culture: place the explants obtained in step (3) in MS anti-browning medium, and culture them under light at 28°C for 16h / d, with light intensity of 50μmol / (m 2 s), 25°C under the condition of dark cultivation for 8h / d, cultured for 40d respectively, MS anti-browning medium is MS basic medium, adding 5.0g / L activated carbon, 35g / L sucrose, 6g / L agar, cultured The pH of the base is 5.7.

[0048] Step (5), rooting culture: place the explants obtained in step (4) in MS rooting medium, and culture them under light at 28°C for 16h / d, with light intensity of 50μmol / (m 2 s), cultivated under the condition of 25°C dark cultivation for 8h / d, and the tobacco took root when cultivated for 9d, and the MS rooting medium was the agar with 5.0g / L activated carb...

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Abstract

The invention relates to a tobacco explant browning prevention method based on activated carbon. The method comprises the following steps of seed sterilization, seed culture, tobacco explant culture,browning prevention culture, rooting culture and hardening-seedling and transplantation. According to the tobacco explant browning prevention method based on activated carbon, the tobacco explant browning phenomenon can be effectively reduced, and the differentiation state of the whole explant can be improved. The root systems grow fast, the texture is pliable, the tobacco is not easily broken, and the survival rate of rooting seedling transplantation is high. The tobacco explant browning prevention method based on activated carbon is convenient to use, low in cost and good in effect.

Description

technical field [0001] The invention relates to a method for preventing browning of tobacco explants, in particular to a method for preventing browning of tobacco explants based on activated carbon, and belongs to the field of plant tissue culture. Background technique [0002] Browning of explants during plant tissue culture is an important factor affecting tissue culture. Studies have shown that the browning of explants is due to the activation of polyphenol oxidase in the tissue, and the phenolic substances are oxidized to produce quinones that inhibit the activity of other enzymes and poison the entire explant tissue. [0003] The current research shows that the causes of explant browning in tissue culture include: plant genotype, explant itself, culture conditions, medium and so on. Although there are many factors that cause browning, choosing appropriate explants, selecting appropriate medium and culture conditions can effectively prevent browning. However, long-term...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李雪梅向海英曾婉俐许力张建铎邓乐乐蒋佳芮宋春满张涛苏杨陈章玉
Owner CHINA TOBACCO YUNNAN IND
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