Tobacco explant browning prevention method based on activated carbon
An explant and activated carbon technology, applied in the field of plant tissue culture, can solve the problems of death and reduce the viability of explants, and achieve the effects of accelerated growth, low cost and improved differentiation state.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] The tobacco explant anti-browning method based on activated carbon of the present embodiment may further comprise the steps:
[0028] Step (1), disinfection of seeds: take plump tobacco seeds, then move them to an ultra-clean workbench, soak the seeds with 75% alcohol for 30s, rinse them twice with sterile water, and then use 1% AgNO 3 Disinfect for 10 minutes, soak and wash in sterile water for 5 times, dry the surface moisture of the explants with sterile filter paper, and then inoculate, wherein the sterile water is ultrapure water sterilized by high pressure.
[0029] Step (2), seed cultivation: inoculate the seeds obtained in step (1) into MS medium, cultivate at a temperature of 25±1°C, and light intensity of 30 μmol / (m 2 s), light time is 16h / d under the condition of cultivating 60d, seed germination grows into seedling, and described seed MS culture medium is the agar that adds 30g / L sucrose and 4g / L in MS basic culture medium, the pH of culture medium The valu...
Embodiment 2
[0038] The tobacco explant anti-browning method based on gac of the present embodiment, wherein:
[0039] Steps (1)-(3) are the same as in Example 1;
[0040] Step (4), anti-browning culture: place the explants obtained in step (3) in MS anti-browning medium, and culture them under light at 28°C for 16h / d, and the light intensity is 30-50μmol / (m 2s), cultivated under the condition of dark cultivation at 25°C for 8h / d for 40d respectively, and the MS anti-browning medium was MS basal medium, adding 2.5g / L activated carbon, 30g / L sucrose, and 4g / L agar, and cultured The pH of the base is 5.7.
[0041] Step (5), rooting culture: put the explants obtained in step (4) in MS rooting medium, and cultivate them under light at 28°C for 16h / d, with light intensity of 45μmol / (m 2 s), cultivated under the condition of 25 DEG C of dark cultivation for 8h / d, and the tobacco took root when cultivating for 5d, and the MS rooting medium was the agar that added 2.5g / L gac, 30g / L sucrose and 4...
Embodiment 3
[0045] The tobacco explant anti-browning method based on activated carbon of the present embodiment may further comprise the steps:
[0046] In steps (2)-(3), the light intensity is 50 μmol / (m 2 s);
[0047] Step (4), anti-browning culture: place the explants obtained in step (3) in MS anti-browning medium, and culture them under light at 28°C for 16h / d, with light intensity of 50μmol / (m 2 s), 25°C under the condition of dark cultivation for 8h / d, cultured for 40d respectively, MS anti-browning medium is MS basic medium, adding 5.0g / L activated carbon, 35g / L sucrose, 6g / L agar, cultured The pH of the base is 5.7.
[0048] Step (5), rooting culture: place the explants obtained in step (4) in MS rooting medium, and culture them under light at 28°C for 16h / d, with light intensity of 50μmol / (m 2 s), cultivated under the condition of 25°C dark cultivation for 8h / d, and the tobacco took root when cultivated for 9d, and the MS rooting medium was the agar with 5.0g / L activated carb...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com