Induction medium for tylophora ovata tissue culture

A technology of induction medium and tissue culture, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of low natural pollination rate of wild resources, less induced differentiation of explants, and damage to the growth environment, etc. Achieve the effect of obvious top advantage, shorten induction culture time, and promote cell division

Inactive Publication Date: 2018-04-03
融安县辉煌农产品种养专业合作社
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Waerteng is used for cancer pain relief among the people in Guangxi, and it has good curative effect. Due to years of excavation, the natural pollination rate of wild resources is low, and the traditional growth environment has been destroyed.
The use of tissue culture technology to serially cultivate the genus genus has become one of the important ways of breeding fine varieties. At present, there are few studies on the tissue culture of the genus genus. Implant induction less differentiation, low induction rate

Method used

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  • Induction medium for tylophora ovata tissue culture

Examples

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Effect test

Embodiment 1

[0018] A kind of induction medium for tissue culture of S. chinensis, adding the following components in 1 / 2MS basal medium: agar 6g / L, brown sugar 12g / L, jasmonic acid 2mg / L, 6-benzylaminoadenine 2mg / L , Brassinosterol 5mg / L, mulberry juice 40mg / L, apple puree 12mg / L, mashed potatoes 15mg / L, hydrolyzed protein 3mg / L.

Embodiment 2

[0020] A kind of induction medium for tissue culture of S. chinensis, adding the following components in 1 / 2MS basal medium: agar 8g / L, brown sugar 18g / L, jasmonic acid 9mg / L, 6-benzylaminoadenine 6mg / L , Brassinosterol 12mg / L, mulberry juice 52mg / L, apple puree 18mg / L, mashed potatoes 25mg / L, hydrolyzed protein 7mg / L.

Embodiment 3

[0022] A kind of inducing culture medium of the vine tissue culture, add following components in 1 / 2MS basal medium: agar 7g / L, brown sugar 15g / L, jasmonic acid 5.5mg / L, 6-benzylaminoadenine 4mg / L L. Brassinosterol 8.5mg / L, mulberry juice 46mg / L, apple puree 15mg / L, mashed potatoes 20mg / L, hydrolyzed protein 5mg / L.

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Abstract

The invention relates to the technical field of culture media for plant seedlings, in particular to an induction medium for tylophora ovata tissue culture. According to the induction medium, the following components are added to 1/2 MS medium: 6-8 g/L of agar, 12-18 g/L of brown sugar, 2-9 mg/L of jasmonic acid, 2-6 mg/L of 6-benzyladenine, 5-12 mg/L of brassinosteroid, 40-52 mg/L of mulberry juice, 12-18 mg/L of mashed apple, 15-25 mg/L of mashed tomato and 3-7 mg/L of protein hydrolysate. The induction medium for tylophora ovata tissue culture performs induction culture on tylophora ovata with seedlings as explants, the dormant state of the explants can be broken, production of seedlings is accelerated, growth of the seedlings is promoted, and the induction rate is increased.

Description

【Technical field】 [0001] The invention relates to the technical field of medium for growing seedlings of plants, in particular to an inducing medium for tissue culture of vines. 【Background technique】 [0002] Tylophora is a perennial grapelike vine-like herb of the genus Tylophora in the family Asclepiadaceac. It is mainly used as medicine with roots and whole plants. It is mainly grown in Yunnan, Guangxi, Guangdong, Hunan, Jiangxi, Zhejiang, Taiwan, Vietnam, Laos, Myanmar, India and other places like warmth, can tolerate shade, and are relatively cold-tolerant. It often grows in mountainous bushes below 900 altitudes, or in valleys and sunny dense forests. The baby's vines are winding, suitable for fences, railings, door frames, and flower galleries, and can also be dotted in the gaps in rockery and rock slopes. Climbing around them, the vines are drooping, which is quite unique. [0003] Waerteng has a long history of being used as medicine in our country. "Jiangxi Herba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 杜乐贵
Owner 融安县辉煌农产品种养专业合作社
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