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Analytic kit for breast cancer chemotherapy

A breast cancer and kit technology, applied in the field of analysis kits after breast cancer chemotherapy, can solve the problem that the evaluation system of breast cancer chemotherapy resistance is not comprehensive enough

Inactive Publication Date: 2018-04-06
NANTONG UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the relevant research at home and abroad focuses on M2 macrophages, which is not comprehensive enough for the evaluation system of breast cancer chemotherapy resistance.

Method used

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  • Analytic kit for breast cancer chemotherapy
  • Analytic kit for breast cancer chemotherapy
  • Analytic kit for breast cancer chemotherapy

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Embodiment Construction

[0013] The following will clearly and completely describe the technical solutions in the embodiments of the present application with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, not all of them. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0014] see figure 1 , figure 1 It is a structural schematic diagram of an embodiment of an analysis kit for breast cancer after chemotherapy in this application. The kit 100 includes: a flow cytometry detection kit 10, a tissue immunohistochemical detection kit 20, and a real-time fluorescent quantitative PCR detection reagent box of 30.

[0015] Among them, the flow cytometry detection kit 10 is used to count the cells of the clinical tissue samples of ...

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Abstract

The invention discloses an analytic kit for breast cancer chemotherapy. The kit comprises a flow cytometry detection kit, a tissue immunohistochemical detection kit and a real-time fluorescent quantitative PCR detection kit, wherein the flow cytometry detection kit is used for counting the number of cells of a clinical tissue sample after chemotherapy of a breast cancer patient to obtain a first drug resistance assessment coefficient of the breast cancer patient after chemotherapy; the tissue immunohistochemical detection kit is used for performing double staining on the clinical tissue sampleto obtain a second drug resistance assessment coefficient of the breast cancer patient after chemotherapy; performing quantitative amplification for the clinical tissue sample with a real-time fluorescent quantitative PCR detection kit to obtain a third drug resistance assessment coefficient of the breast cancer patient after chemotherapy; and calculating the drug resistance index of the breast cancer patient after chemotherapy through the first drug resistance assessment coefficient, the second drug resistance assessment coefficient and the third drug resistance assessment coefficient. By adopting the method, the product support is provided for comprehensive and complement assessment on the drug resistance situation of the breast cancer patient after chemotherapy.

Description

technical field [0001] The application relates to the technical field of rapid detection kits, in particular to an analysis kit for breast cancer after chemotherapy. Background technique [0002] Breast cancer is one of the most common malignant tumors in women. According to official data, in 2015, 268,000 women were newly diagnosed with breast cancer in my country, and about 69,500 women died of breast cancer. In recent years, advances in medicine have reduced cancer morbidity and mortality to some extent, but many people are still diagnosed with cancer. [0003] Breast cancer is a tumor that is moderately sensitive to chemotherapy, and chemotherapy plays an important role in the treatment of breast cancer. The drug resistance of cancer cells during chemotherapy leads to short-term recurrence or metastasis in many subjects after chemotherapy, which is one of the reasons for the high mortality rate of breast cancer subjects. Therefore, revealing the relevant cellular and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N33/50G01N33/53G01N33/68C12Q1/6886
CPCC12Q1/6886C12Q2600/158G01N15/14G01N33/5055G01N33/5302G01N33/68
Inventor 王国华高纯一姜正林徐丽华王金星李霞
Owner NANTONG UNIVERSITY
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