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Primer pair composition and probe for simultaneously detecting 10 types of arthropod-borne viruses and application

An arbovirus and primer pair technology, which is applied in the direction of microorganisms, recombinant DNA technology, and methods based on microorganisms, can solve the problems of complex operation of detection methods, narrow analysis range, poor repeatability, etc., and achieve fast detection speed and high sensitivity , the effect of stable results

Inactive Publication Date: 2018-04-10
GUIZHOU MEDICAL UNIV
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Problems solved by technology

[0007] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide primer pair combinations, probes and applications for simultaneous detection of 10 arboviruses, aiming to solve the problem of complex operation, high cost, poor repeatability and sensitivity of existing detection methods. Low, narrow analysis pan and other issues

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  • Primer pair composition and probe for simultaneously detecting 10 types of arthropod-borne viruses and application
  • Primer pair composition and probe for simultaneously detecting 10 types of arthropod-borne viruses and application
  • Primer pair composition and probe for simultaneously detecting 10 types of arthropod-borne viruses and application

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Embodiment Construction

[0058] The present invention provides primer pair combinations, probes and applications for simultaneous detection of 10 arboviruses. In order to make the purpose, technical solutions and effects of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0059] (1) Detection of virus types

[0060] The present invention determines the list of viruses detected according to the pathogenicity of the virus, global influence, public health significance, risk of introduction and epidemic situation, etc., a total of 10 kinds (types) of viruses, and the detailed information of various viruses is shown in Table 1.

[0061] Table 1 Classification of viruses, diseases caused by them, and animal vectors

[0062]

[0063] (2) Design of fluorescent quantitative RT-PCR primers and Taq...

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Abstract

The invention discloses a primer pair composition and probe for simultaneously detecting 10 types of arthropod-borne viruses and application. The primer pair composition and probe have sequences shownin SEQ ID NO:1-30. The primer pair composition has the amplification efficiency of 87.20-102.00%. It is shown through sensitivity, repeatability and specificity detection results of all systems thatthe sensitivity is 101-102 copies per microliter and the inside-composition variable coefficient is smaller than 3%; it is verified through cross reactions among primer-probe pairs of 10 types of viruses that all the primer-probe pairs and other viruses have no remarkable amplification. The primer pair composition and probe system is high in sensitivity, strong in specificity, stable in result, good in repeatability, simple in operation and rapid in detection speed and can rapidly and accurately detect the 10 types of arthropod-borne viruses.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to primer pair combinations, probes and applications for simultaneous detection of 10 arboviruses. Background technique [0002] Arboviruses refer to some blood-sucking arthropods (mosquitoes, ticks, etc.) that bite sensitive vertebrates (birds, bats, primates, livestock, etc.) sick bunch of viruses. The diseases caused by arboviruses are distributed all over the world. Studies on arboviruses have found that in recent decades, some arbovirus diseases that have been effectively controlled have reappeared locally and newly appeared in other areas, and The epidemic has expanded globally, and cases have risen sharply. The morbidity, mortality, and risk of introduction of these diseases are relatively high, and they are still important global public health problems. [0003] Globally and in combination with my country's subtropical regions, there are mainly 10 arboviruses that have...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11C12R1/93
CPCC12Q1/6851C12Q1/701C12Q2531/113C12Q2561/101
Inventor 吴家红
Owner GUIZHOU MEDICAL UNIV
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