The invention discloses a
recombinant antigen protein for detecting a dengue 2 type
virus antibody, a kit and an application of the
recombinant antigen protein. The preparation method of the
recombinant antigen protein comprises the following steps of screening and detecting a target coding
gene, then connecting the coding
gene into an
prokaryotic expression vector to construct a
recombinant expression vector; and converting the
recombinant expression vector into a host
cell, and carrying out inducing expression to obtain the recombinant
antigen protein DENV-Ag2, wherein the recombinant
antigen protein is a dengue 2 type
virus specific
antigen, and is obtained by
prokaryotic expression and purification. The recombinant antigen protein disclosed by the invention has the advantages of beingstrong in specificity and high in affinity, has very high affinity with dengue 2 type
virus antibody, and does not have a serum cross reaction with other dengue viruses and similar arboviruses. The kit provided by the invention has the advantages of simplicity and convenience in operation, high sensitivity, strong specificity and the like, can rapidly and accurately identify the
dengue virus 2 type virus
antibody, so as to diagnose the infection condition of
dengue virus, thereby providing favorable support for subsequent treatment.