30 results about "Arbovirus" patented technology

Provided herein are alphavirus vectors derived from South African Arbovirus No. 86 (S.A.AR86) comprising attenuating mutations and methods of making the same. Also provided are improved viral vectors and helper constructs comprising a S.A.AR86 capsid enhancer sequence. The present invention also provides S.A.AR86 replicon and helper constructs comprising an alphavirus capsid enhancer sequence. Further provided are methods of administering an alphavirus vector comprising a heterologous nucleotide sequence (preferably encoding an immunogen or a therapeutic polypeptide) according to the invention to a cell or subject. In preferred embodiments, the alphavirus vector delivers the heterologous nucleotide sequence to the cells of the bone, bone marrow, and / or bone-associated connective tissue.

The invention discloses Marburg and Ebola dual-virus fluorescent quantitative PCR (Polymerase Chain Reaction) detection method and system, wherein the detection system comprises primers, probes, a Premix EX Taq reaction solution and sterilizing Tris water. As two pairs of primers and probes have very good specificity, the detection system has high sensitivity and is suitable for simultaneously detecting Marburg and Ebola viruses without having cross reaction with other kinds of hemorrhagic fever arbovirus, such as yellow fever, dengue and rift valley fever.

The invention discloses a primer pair composition and probe for simultaneously detecting 10 types of arthropod-borne viruses and application. The primer pair composition and probe have sequences shownin SEQ ID NO:1-30. The primer pair composition has the amplification efficiency of 87.20-102.00%. It is shown through sensitivity, repeatability and specificity detection results of all systems thatthe sensitivity is 101-102 copies per microliter and the inside-composition variable coefficient is smaller than 3%; it is verified through cross reactions among primer-probe pairs of 10 types of viruses that all the primer-probe pairs and other viruses have no remarkable amplification. The primer pair composition and probe system is high in sensitivity, strong in specificity, stable in result, good in repeatability, simple in operation and rapid in detection speed and can rapidly and accurately detect the 10 types of arthropod-borne viruses.

The invention discloses a triple detection method of arbovirus liquid chips. The method comprises the following steps: respectively designing specific primers and probes aiming at three types of arboviruses comprising Chikungunya viruses, Crimean-Congo hemorrhagic fever and Rift Valley fever viruses, determining the specificity of the primers and the probes via a single PCR and clone sequencing, and then carrying out multiplex-PCR amplification under the condition that the concentration ratio of the forward and reverse primers in an amplification reaction system is 1:1-1:8; then coupling the probes with fluorescent coding microspheres so as to prepare a microsphere mixing liquid and carrying out coupling quality control by coupling the probes; finally, carrying out a hybridization reaction on multiplex-PCR products and the microsphere mixing liquid, simultaneously adding streptomycin-phycoerythrin diluted by a nucleic acid detection buffer solution, and analyzing reaction products by utilizing a liquid chip detecting instrument. The method is good in detection specificity and can be used for simultaneously detecting the three types of arboviruses, and has high detection sensitivity due to the adoption of an asymmetric PCR amplification method in the process of the multiplex-PCR amplification.

The present disclosure relates to methods for inactivating a Zika virus which can be used in vaccines and immunogenic compositions. The present disclosure also relates to a method for determining the completeness of inactivation of an arbovirus preparation and to a method for determining the residual formaldehyde content in a pharmaceutical composition comprising an inactivated virus.

An essential oil blend diluted in soybean oil against the bites of Anopheles stephensi, An. gambiae (the main malaria vectors in Asia and Africa, respectively); Aedes aegypti and Ae. albopictus (the main vectors of arboviruses listed above). Repellency tests, appraising the protection time for each species, were performed under laboratory conditions using human volunteers and nulliparous female mosquitoes. The results show that the natural repellent was extraordinarily effective against both Anopheles and Aedes genera.

The invention discloses real-time fluorescence quantification PCR (polymerase chain reaction) detection primers for a novel goose arbovirus which causes gout of goslings, and a kit of the primers. Thekit comprises an upstream primer GoAstV-F1 and a downstream primer GoAstV-R1. The kit disclosed by the invention is capable of detecting the novel goose arbovirus which causes gout of the goslings, has the advantages of being good in specificity, good in repeatability and accurate in quantification, is good in sensitivity, is capable of detecting 6.8 copies at least, has a very good linear relationship within a 6.88-6.88*10<9> copy range of a standard product, has a detection range up to 10 orders of magnitudes, is capable of rapidly detecting the novel goose arbovirus GoAstV which causes gout of the goslings, and improves the detection efficiency.

Described herein are compositions and methods for treating a disease, particularly a cancer, with an immune checkpoint modulatory agent and a strain of an Arbovirus or a strain of an Alphavirus. Also provided herein are also methods for combination therapy comprising administration of an immune checkpoint modulatory agent, tumor antigen primed dendritic cells and an Alphavirus or an Arbovirus.

The present disclosure relates to methods for inactivating a Zika virus which can be used in vaccines and immunogenic compositions. The present disclosure also relates to a method for determining the completeness of inactivation of an arbovirus preparation.

The invention discloses a method for detecting Japanese encephalitis virus, Dengue virus and Chikungunya virus based on a hyper-branched rolling cycle amplification nucleic acid test strip, and belongs to the field of entry-exit health inspection and quarantine. The method includes the following steps: (1) designing lock probes and general primers for all the viruses; (2) optimizing the conditionsfor hyper-branched rolling cycle amplification, and establishing a specific and accurate hyper-branched rolling cycle amplification detection technology system for the Japanese encephalitis virus, Dengue virus and Chikungunya virus; and (3) crosslinking the three arboviruses detection probes to a cellulose nitrate membrane to achieve specific detection of viral hyper-branched rolling cycle amplification products, and successfully developing the hyper-branched rolling cycle amplification test strip for detecting various arboviruses. The specificity and sensitivity of detection are ensured, thedetection time is shortened and the custom clearance speed is improved, and the test strip is of great significance for improving interception of entry health epidemics, protecting safety of lives ofChinese people, speeding up the custom clearance speed at ports and reducing the cost of detection.

The invention discloses a recombinant antigen protein for detecting a dengue 2 type virus antibody, a kit and an application of the recombinant antigen protein. The preparation method of the recombinant antigen protein comprises the following steps of screening and detecting a target coding gene, then connecting the coding gene into an prokaryotic expression vector to construct a recombinant expression vector; and converting the recombinant expression vector into a host cell, and carrying out inducing expression to obtain the recombinant antigen protein DENV-Ag2, wherein the recombinant antigen protein is a dengue 2 type virus specific antigen, and is obtained by prokaryotic expression and purification. The recombinant antigen protein disclosed by the invention has the advantages of beingstrong in specificity and high in affinity, has very high affinity with dengue 2 type virus antibody, and does not have a serum cross reaction with other dengue viruses and similar arboviruses. The kit provided by the invention has the advantages of simplicity and convenience in operation, high sensitivity, strong specificity and the like, can rapidly and accurately identify the dengue virus 2 type virus antibody, so as to diagnose the infection condition of dengue virus, thereby providing favorable support for subsequent treatment.

Compositions and methods are provided for preventing or treating a human suffering from a meningitis, encephalitis, meningo-encephalitis or encephalomyelitis caused by an arbovirus, comprising parenterally administering to the human an effective amount of interferon.

The invention discloses a non-diagnostic method for detecting flavivirus by a TaqMan probe through real-time fluorescent reverse transcription-polymerase chain reaction (RT-PCR). The non-diagnostic method comprises one pair of primers and the probe which are used for performing real-time quantitative detection on a sample to be detected. The invention aims to solve the problem that convenience is brought to monitoring and detecting emerging arboviruses because the flavivirus has multiple members and the detection is complicated. The defects existing in traditional and modern methods are overcome by the non-diagnostic method. The primers and the probe have the characteristics that the specificity is high, the repeatability is high, the sensitivity is high and multiple quantitation can be performed.

An essential oil blend diluted in soybean oil against the bites of Anopheles stephensi, An. gambiae (the main malaria vectors in Asia and Africa, respectively); Aedes aegypti and Ae. albopictus (the main vectors of arboviruses listed above). Repellency tests, appraising the protection time for each species, were performed under laboratory conditions using human volunteers and nulliparous female mosquitoes. The results show that the natural repellent was extraordinarily effective against both Anopheles and Aedes genera.

The invention discloses an immunodiagnosis kit for specifically detecting a chikungunya virus NSP1 antigen. The diagnostic kit comprises a microporous reaction plate coated with a monoclonal antibodyCHIKV-M2, a sample treatment solution, a monoclonal antibody CHIKV-M10 combined with a marker, positive control, negative control, a concentrated washing solution, a developing solution and a stop solution. The kit can specifically detect the chikungunya virus antigen, has no cross reaction with other arbovirus antigens, and detects chikungunya virus culture supernatant sensitivity similar to thatof a commercial fluorescent quantitative PCR detection kit.

The invention discloses a non-diagnostic method for detecting flavivirus and alphavirus through double TaqMan probe real-time fluorescence RT-PCR. The non-diagnostic method comprises real-time and quantitative detection carried out on a sample to be detected through designing complement sequences for an NS5 gene in the flavivirus sequence and an NS1 gene in the alphavirus sequence, and technologies of conventional retro-transcript PCR amplicaiton and real-time quantitative RT-PCR are adopted; therefore, the arbovirus detection process is simplified, the virus isolating technology with defectsof complex operation, great technical difficulty and long experiment time is replaced, and the biosecurity during detection is improved. According to the invention, the high-flux, quick and sensitivemethod for detecting and screening flavivirus and alphavirus is established and plays an important role in detecting the arboviuses.

The invention belongs to the technical field of virus detection, and discloses a rapid detecting primer set and kit of a GeXP for detecting four kinds of flaviviruses simultaneously and application ofthe rapid detecting kit. Based on the GeXP technology, four pairs of specific primers and a pair of universal primers are designed, a multi-PCR system is constructed on this basis, Zika viruses, yellow fever viruses, dengue viruses and west nile viruses can be detected at the same time, the detecting time is effectively shortened, the advantages of being high in specificity, high in sensitivity,high in accuracy, convenient, efficient and the like are achieved, and the rapid detecting primer set and kit have important significance on diagnosis, prevention and control of insectborne infectiousdisease viral diseases caused by the four kinds of viruses.

An improved method of inducing protective immunity to the Human Immunodeficiency Virus through isolating a person's antigen-presenting cells from blood, then pulsing these cells with short peptides that bind the person's Class I Major Histocompatibility Complex Types and correspond to conserved segments of the HIV structural and functional genes. These pulsed dendritic cells are then injected intravenously, where they will travel to lymph tissue and prime HIV-specific Cytotoxic T Lymphocytes. The immune system is then activated by injecting the person with a weakened arbovirus, Chikungunya. The interferons and other immune-activating chemicals induced by the virus will stimulate creation of billions of killer cells that recognize HIV proteins, allowing for rapid mobilization of memory immune cells to contain and eliminate any subsequent infection with the HIV virus. The vaccine is custom-designed to each person and uses no HIV virus for safety purposes. It is understood that the examples an embodiments described herein are for illustrative purposes only and various changes or modifications in light thereof will be suggested to persons skilled in the art and are to be included within the spirit and purview of this application and scope of the appended claims.

Described herein are compositions and methods for treating a disease, particularly a cancer, with an immune checkpoint modulatory agent and a strain of an Arbovirus or a strain of an Alphavirus. Also provided herein are also methods for combination therapy comprising administration of an immune checkpoint modulatory agent, tumor antigen primed dendritic cells and an Alphavirus or an Arbovirus.

The present disclosure provides vaccine compositions for prophylaxis and treatment of Zika virus infections comprising Zika virus antigens in immunogenic compositions, and in combination of Zika antigens with one or more arbovirus antigens such as Chikungunya virus and Japanese encephalitis virus antigens, methods of preparation and production of such compositions for use as vaccines for eliciting immune response in mammals against the above mentioned pathogens.

The invention discloses a probe, a primer, a detection kit and a detection method for detecting arboviruses based on a suspension microsphere array system. According to the technical scheme, the probefor detecting the arboviruses is coupled with different coding microspheres, a suspension microsphere array is obtained after mixing, the specific primer provided by the invention is then used for carrying out multiple RT-PCR amplification on a sample to be detected, the PCR product is in hybridization with the suspension microsphere array, streptavidin R- phycoerythrin is then used for carryingout developing, then a suspension microsphere array reading instrument is used for reading the detection result, and that whether the sample to be detected contains the detected arboviruses or not isjudged. The probe and the primer for detecting the arboviruses based on the suspension microsphere array system can simultaneously detect 7 kinds of arboviruses, thus the detection time is greatly shortened, and the detection efficiency and accuracy are improved.

The invention discloses a probe, a primer, a detection kit and a detection method for detecting insect vector viruses based on a suspended microbead array system. The present invention couples the probes for detecting arboviruses with different coded microbeads respectively, mixes them to obtain a suspended microbead array, and then uses the specific primers of the present invention to perform multiple RT-PCR amplification on the samples to be tested, and PCR The product is hybridized with the suspended microbead array, and then the streptavidin R-phycoerythrin is used for color development, and the detection result is read by the suspended microbead array reader to determine whether the detected insect vector is contained in the sample to be tested Virus. The probes and primers for detecting arboviruses based on the suspended microbead array system of the present invention can simultaneously detect seven kinds of arboviruses, can greatly shorten the detection time, and improve detection efficiency and accuracy.

The invention discloses application of isoretinoic acid in preparation of a product for controlling transmission of arbo-viruses and a method of the isoretinoic acid. The invention provides any one of the following applications (i)-(iii) of isoretinoic acid or pharmaceutically acceptable salts thereof: (i) an application in preparation of a product for controlling transmission of arbo-viruses; (ii) an application in preparation of a product for inhibiting the susceptibility of a transmission medium to an arthrovirus; and (iii) application in preparation of a product for reducing contact between a transmission medium and a host. It is found that by applying isoretinoic acid to the host, the efficiency of biting the host by a transmission medium can be reduced, for example, the susceptibility of the transmission medium to the arbo-viruses is inhibited, contact between the transmission medium and the host is reduced, and therefore transmission of the arbo-viruses is inhibited or blocked.