68results about How to "Improve sequence" patented technology

Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.

Methods and systems of DNA sequencing that compensate for sources of noise in next-generation DNA sequencers are described.

A traitor tracing enabling system assigns sequence keys to a media player to enable better tracability with fewer actual variations in the content. The system comprises a technique for increasing a number of sequence keys, allowing a finer granularity in a traitor tracing process with little increase in sequence key storage or in bandwidth required to transmit sequence keys to a media player. The present system comprises a variation table which maps the sequence keys to the individual variations in the file, such that multiple sequence keys can be mapped to a single variation.

Provided herein are alphavirus vectors derived from South African Arbovirus No. 86 (S.A.AR86) comprising attenuating mutations and methods of making the same. Also provided are improved viral vectors and helper constructs comprising a S.A.AR86 capsid enhancer sequence. The present invention also provides S.A.AR86 replicon and helper constructs comprising an alphavirus capsid enhancer sequence. Further provided are methods of administering an alphavirus vector comprising a heterologous nucleotide sequence (preferably encoding an immunogen or a therapeutic polypeptide) according to the invention to a cell or subject. In preferred embodiments, the alphavirus vector delivers the heterologous nucleotide sequence to the cells of the bone, bone marrow, and/or bone-associated connective tissue.

The invention relates to an optimization method for data layout. The optimization method comprises the following steps: 1, receiving continuous data flow input by a service system; 2, dividing the data flow into a plurality of continuous data blocks without intersection; 3, judging whether the data blocks to be processed reach the upper limit of caching, if yes, carrying out the next step, and if not, skipping to the step 1; 4, obtaining fingerprints of all the data blocks in caching through calculation; 5, matching the fingerprints of the data blocks in caching and fingerprints in a fingerprint list, marking the matched data blocks as repeated data blocks, and storing the fingerprints which are not matched into the fingerprint list; 6, upgrading the physical location information of the data blocks in a data block information table; and 7, storing the corresponding data blocks according to the physical location information of the data blocks in the data block information table. According to the optimization method, the sequence of data is improved, discretization of data layout is lowered, and the sequential reading and writing performance of a repeated data deleting system is improved.

The present invention relates to a process for the back-surface grinding of wafers using films which have a support layer, which is known per se, and an adhesion layer which can be polymerized in steps, and to films which include such an adhesion layer which can be polymerized in steps, and to the use thereof.

A sequence program editing apparatus includes a program storing unit for storing an application program including a sequence program, an information storing unit for storing symbol/comment information used in the sequence program, a selecting unit for selecting a part or a whole of a sequence program displayed on an application screen run by the first application program, an extracting unit for extracting the symbol/comment information included in the selected sequence program from the information storing unit, and a copy unit for copying the selected sequence program on the application screen run by the second application program and copying the extracted symbol/comment information on the information storing unit of the second application.

The invention relates to an SMR disk drive with hybrid E-regions, including a nonvolatile solid state memory E-region in addition to magnetic media E-regions on the disk. The memory E-region can be used in operations that can be referred as destaging and/or restaging to sequentialize sets of exception records to reduce the time and energy spent in executing seeks in the disk E-region. The ratio of the size of the solid state memory E-region to the total E-region capacity on the disks can be optimized for selected applications according to the invention using tradeoffs between performance and cost. For example, an embodiment with a memory E-region size that is 10% of the total disk E-region capacity achieves substantial performance enhancement over a disk-only E-region implementation and also results in smaller costs than can be required in a NAND-only memory E-region.

The invention relates to the simultaneous analysis of variations in distinct nucleic acid sequences within a complex nucleic acid mixture. The invention consists in the use of chips with spatially separated fields of photocleavable oligonucleotide probes which are commonly processed together with the target sequences and thereby modified in a sequence-dependent way. The probes are cleaved and analyzed by laser desorption mass spectrometry. This allows highly parallel and nevertheless sequence-specific reactions within a single reaction mixture. Since the photocleavable oligonucleotide probes are placed on a substrate in a spatially defined manner, e.g. in the form of an array, a specific target sequence can be assigned a unique position on the oligonucleotide chip. Since the photocleavable probes are covalently immobilized on the solid surface, the detection reaction can be performed directly on the chip while preserving the probe position pattern. Due to the presence of a photosensitive residue within the probes, these can be controlled photolytically after the detection reaction so that a mass spectroscopic analysis can then be carried out.

A flip-flop having improved set-up time and a method used with are provided. The flip-flop comprises a first master latch, a first selector, a second master latch, a second selector, and a slave latch. The first master latch receives the critical data and is used to latch the critical data. The first selector receives a plurality of non-critical data and outputs a first selected data to the second latch. The second master latch is used to latch the first selected data. The second selector is coupled to the first master latch and the second master latch in order to output a second selected data to the slaver latch. The slave latch is used to latch and output the second selected data.

A motor vehicle door lock (3) provided for connection to a vehicle door, in which the motor vehicle door lock (3) comprises a carrier plate (4) whereon the locking pieces (2, 21, 22) are mounted, and a lock housing (32) which at least partially surrounds the locking pieces (2, 21, 22) comprising the carrier plate (4), comprising a counter piece (34) formed from the side (36) of the lock housing (32), which is opposite to the carrier plate (4), the counter piece (34) being provided in such a way that it co-operates with a connection element (7) in the vehicle door (8) by means of a through opening (43) in the carrier plate (4).

The invention relates to traditional Chinese medicine decoction piece uniformly spreading and drying equipment and a using method thereof, and belongs to the technical field of traditional Chinese medicine processing equipment. The equipment comprises conveying equipment, wherein a drying box is arranged on the rear side of the conveying equipment, and a spreading mechanism is arranged above the front side of the conveying equipment; and a discharging hopper is arranged above the spreading mechanism, and a 2mm-4mm distance is formed between the discharging hopper and the spreading mechanism. The using method comprises the following three steps of initially adjusting, starting a drying box, and starting a motor I and a motor and the like. According to the using method, the distance betweena baffle ring and the surface of a shell body is determined according to the thickness of the traditional Chinese medicine decoction piece, and the situation that only one traditional Chinese medicinedecoction piece can pass through the space between the baffle ring and the shell body one time prevails; adjustment of the distance between the discharging hopper and the spreading mechanism is subjected to the situation that the traditional Chinese medicine decoction pieces are not stacked and blocked on the spreading mechanism; and the lengths of cylinders are adjusted, so that distance betweenthe lower ends of the cylinders and a conveying belt does not exceed the thickness of the two traditional Chinese medicine decoction pieces, and the influence of the cylinders on conveying of the traditional Chinese medicine decoction pieces is avoided.

The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns.

A tool handling device for machine tools, comprising a tool magazine having a transfer position for the transfer of the tools, including a tool changer movable between the transfer position and an exchange position, which has a rotatable double gripper, and a supporting structure movable on a foundation, on which the tool changer is mounted. In order to achieve fast tool changes in tight spaces on the machine, the supporting structure has an upper slide and a lower slide which can both be displaced by means of spindle drives in the same direction on a foundation, wherein a single drive motor is provided for driving the two spindle drives.

The invention discloses a rear axle processing machine tool, which comprises a machine tool body. A portal frame is arranged on the machine tool body; a workbench driven by a first power device and sliding along an X axle is also arranged on the machine tool body; a saddle driven by a second power device is slidably arranged along a Y axle and located on the portal frame above the workbench; a slide seat driven by a third power device is slidably arranged on the saddle along a Z axle; a first spindle driven by a fourth power device is arranged on the slide seat; and end face processing mechanisms arranged in a mirroring way are arranged on two sides of the machine tool body, and are located on one side, provided with the first spindle, on the portal frame. The simultaneous processing of two upper ends and three middle holes on a rear axle can be completed through the one machine tool, so that compared with a traditional rear axle processing machine tool, the problem of requiring the matching of multiple machine tools and multiple clamping is solved, a lot of rear axle transfer time is saved, and the working efficiency is greatly improved; and meanwhile, the rear axle only needs to be clamped and positioned at a time, so that the location degree of the rear axle during processing is ensured, and further the processing accuracy is effectively improved.

The present invention relates to a receiver apparatus and method of channel estimation in a telecommunication system which provides at least two pilot sequences, and to a computer program product. Channel estimation is achieved by estimating channel taps separately for each of the at least two pilot sequences in every transmission block, and for applying estimated channel taps obtained from the estimation to at least one of a temporal and spatial filtering or combining operation to refine the channel estimate. Accordingly, temporal correlations and cross-correlations of the at least two pilot sequences are exploited without requiring knowledge of path delays and beamforming parameters.

The invention relates to a casting method of aluminum alloy castings. The casting method of aluminum alloy castings comprises the following steps of firstly, making a cope flask and a drag flask according to the shape of one casting, and carrying out coating treatment on the cope flask and the drag flask; secondly, carrying out mold assembly on the cope flask and the drag flask to form a cavity, and carrying out fastening treatment; thirdly, carrying out vacuum pumping treatment on the cavity; fourthly, filling the cavity with aluminum alloy molten metal with the injection speed ranging from 2m/s to 6m/s; fifthly, increasing the pressure of the cavity to 5 Mpa to 10 Mpa, and carrying out pressure maintaining treatment; sixthly, relieving the pressure of the cavity to the normal pressure after the casting formed in the cavity completely solidifies, separating the cope flask from the drag flask, and taking out the casting, namely, the aluminum alloy casting. The aluminum alloy castings prepared with the preparation method is good in surface quality and low in preparation cost.

The invention concerns the field of recombinant gene engineering. It concerns novel introns and compositions comprising such introns as well as a method to improve expression of polypeptides from nucleic acids such as cloned genes with heterologous introns, especially genes encoding antibodies and antibody derived fragments, and the production of various polypeptides in eukaryotic host cells using said novel intron sequences as heterologous introns.

A method for transacting nucleic acid to cell comprising a step for forming a nucleic acid complex by bringing a double-stranded nucleic acid molecule into contact with a nucleic acid carrier having an amino acid sequence of alternating a basic amino acid and a hydrophobic amino acid, which has a peptide chain that forms a β-sheet structure in which a side chain of a positively charged basic amino acid is disposed on one surface side and a side chain of a hydrophobic amino acid is disposed on the opposite surface side in the presence of the double-stranded nucleic acid molecule having a double helix structure, and by binding the double-stranded nucleic acid molecule and the peptide chain through either one or both of the electrostatic interaction between the side chains of the basic amino acid and phosphate groups and hydrogen bonds between the double stranded-nucleic acid molecule and the peptide chain and a nucleic acid complex used for the same are disclosed.

The invention discloses a low-latency file system address space management method and system and a medium. The method comprises the steps that a super block and a block group distribution table are generated from an address space of storage equipment, file system information and the distribution situation of a block group on a linear address space of the storage equipment are stored in the super block, and the block group distribution table is used for marking the distribution situation of data blocks in the corresponding block group; when the file is created, dynamically creating or selectinga corresponding block group according to the size of a specified data block, allocating the data block, writing file data into the allocated data block, and updating the information of a block groupallocation table and a super block. It can be guaranteed that which data blocks are occupied by one file on the storage device can be known only through one IO operation, the read-write delay of the file can be remarkably reduced, the addressing expenditure of file read-write can be remarkably reduced, the file read-write sequence can be remarkably improved, and therefore the read-write performance is improved.

A radio communication system (10A) has a transmission system (20A) and a radio terminal (300). The transmission system (20A) superimposes and transmits a delay stream (S1_delay) and a communication stream (S1), and superimposes and transmits a delay stream (S2_delay) having a different delay than the delay stream (S1_delay) and a communication stream (S2). A transmission system (20A) notifies delay information (S1_info) about the delay stream (S1_delay) and delay information (S2_info) about the delay stream (S2_delay). The radio terminal (300) combines the communication stream (S1) and the delay stream (S1_delay) in accordance with the notified delay information (S1_info), and combines the communication stream (S2) and the delay stream (S2_delay) in accordance with the notified delay information (S2_info).