A probe, primer, detection kit and detection method for detecting arboviruses based on a suspended microbead array system

Abstract

The invention discloses a probe, a primer, a detection kit and a detection method for detecting insect vector viruses based on a suspended microbead array system. The present invention couples the probes for detecting arboviruses with different coded microbeads respectively, mixes them to obtain a suspended microbead array, and then uses the specific primers of the present invention to perform multiple RT-PCR amplification on the samples to be tested, and PCR The product is hybridized with the suspended microbead array, and then the streptavidin R-phycoerythrin is used for color development, and the detection result is read by the suspended microbead array reader to determine whether the detected insect vector is contained in the sample to be tested Virus. The probes and primers for detecting arboviruses based on the suspended microbead array system of the present invention can simultaneously detect seven kinds of arboviruses, can greatly shorten the detection time, and improve detection efficiency and accuracy.

Description

A probe, primer and detection method for arbovirus detection based on suspension microbead array system Test kit and detection method technical field The invention belongs to the field of molecular biology, be specifically related to a kind of detection insect-borne disease based on suspension microbead array system Toxic probes, primers, detection kits and detection methods. Background technique Arbovirus refers to a class of blood-sucking insects as a medium, which spreads a variety of serious diseases among vertebrates and humans and animals. sick virus. In recent years, dengue virus and Zika virus infection have been prevalent in tropical jungle areas of the world and in southern my country. It is an insect-borne virus. Patients infected with arboviruses have similar clinical symptoms, including fever, muscle Flesh and joint pain, rash, swollen lymph nodes, etc. At present, the main clinical methods are enzyme-linked immunosorbent assay or fluorescence quant...

AI Technical Summary

Problems solved by technology

[0006] Although the two suspended microbead array technologies have different encoding technologies, they can both meet the requirements of multiple detection indicators in one test, easy automatic operation, and large sample volume per unit time for clinical applications.

Images