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Immunodiagnosis kit for specifically detecting chikungunya virus NSP1 antigen

A technology for chikungunya virus and immunodiagnosis, applied in the field of medicine, can solve problems such as high requirements, dependence on early sampling and storage conditions, false positive results, etc., and achieve high sensitivity.

Active Publication Date: 2020-07-10
中国人民解放军南部战区疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Virus isolation, through sensitive cell isolation or intracranial inoculation in suckling mice or mosquito inoculation, etc., has the following disadvantages: high requirements for operators and laboratory conditions, relying on early sampling and good storage conditions; in addition, it takes a long time, at least a week
The disadvantage is that there are cross-epitopes between arboviruses, and it is easy to cross-react with other arboviruses to produce false positive results

Method used

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  • Immunodiagnosis kit for specifically detecting chikungunya virus NSP1 antigen
  • Immunodiagnosis kit for specifically detecting chikungunya virus NSP1 antigen
  • Immunodiagnosis kit for specifically detecting chikungunya virus NSP1 antigen

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Embodiment Construction

[0017] The specific implementation of the present invention will be further described below in conjunction with examples, but the implementation and protection of the present invention are not limited to this. It should be pointed out that if there are processes that are not specifically described in detail below, those skilled in the art can implement or understand with reference to the prior art. If the manufacturer of the reagent or instrument is not indicated, it is regarded as a conventional product that can be purchased commercially.

[0018] 1. The preparation methods and identification results of the monoclonal antibodies CHIKV-M2 and CHIKV-M10:

[0019] (1) Preparation of immune antigen

[0020] The immunogen used for preparing the monoclonal antibody of the present invention is the genetically recombinant CHIKV NSP1 protein and the inactivated natural virus antigen. The recombinant CHIKV NSP1 protein is prepared by an engineered strain carrying the CHIKV NSP1 gene. The pr...

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Abstract

The invention discloses an immunodiagnosis kit for specifically detecting a chikungunya virus NSP1 antigen. The diagnostic kit comprises a microporous reaction plate coated with a monoclonal antibodyCHIKV-M2, a sample treatment solution, a monoclonal antibody CHIKV-M10 combined with a marker, positive control, negative control, a concentrated washing solution, a developing solution and a stop solution. The kit can specifically detect the chikungunya virus antigen, has no cross reaction with other arbovirus antigens, and detects chikungunya virus culture supernatant sensitivity similar to thatof a commercial fluorescent quantitative PCR detection kit.

Description

Technical field [0001] The invention relates to the field of medicine, in particular to an immunodiagnostic kit for specifically detecting the NSP1 antigen of Chikungunya virus. Background technique [0002] Chikungnya virus (chikungnya virus, CHIKV) belongs to the Togavirus family alphavirus, a single-stranded positive-stranded RNA arbovirus, and the main vector is Aedes mosquitoes. It can cause chikungunya fever. The main clinical manifestations are sudden high fever, chills, rash, severe joint pain, etc. Some patients turn into chronic infections, manifesting persistent polyarthritis, causing the infected people to lose labor, resulting in Serious social and economic losses. The virus is mainly prevalent in Africa, South America, South Asia and Southeast Asia, and the epidemic area is increasing year by year. Yunnan of my country is also a natural foci of Chikungunya disease, and many imported cases have been detected in recent years. At present, the population of our count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N2333/181G01N2469/10Y02A50/30
Inventor 陈月任瑞文刘乐斌陈荣华刘军张锦海
Owner 中国人民解放军南部战区疾病预防控制中心
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