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Thin-layer chromatography identification method for ant lions

A technique of thin-layer chromatography and reference medicinal materials, applied in the field of traditional Chinese medicine identification, can solve problems such as reports, no identification method of the sand cattle, and achieve the effects of simple equipment, good durability and improved accuracy

Active Publication Date: 2018-05-01
广西壮族自治区食品药品检验所
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Problems solved by technology

Sand Niu has high medicinal value and is also the main component of Wulin Huashi Pills, but there is no report on the identification method of Sand Niu so far

Method used

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  • Thin-layer chromatography identification method for ant lions
  • Thin-layer chromatography identification method for ant lions
  • Thin-layer chromatography identification method for ant lions

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Experimental program
Comparison scheme
Effect test

Embodiment

[0032] Embodiment, content is as follows:

[0033] (1) A total of 11 batches of Sha Niu samples were collected. For details, see Table 1 and Figure 1~3 ;

[0034] Table 1 List of sand cattle sample information

[0035]

[0036] The sand cow sample SN-2 was identified by Zhao Weibo, an entomologist at Guangzhou University of Traditional Chinese Medicine, as the dried larvae of the insect class Antflyidae.

[0037] (2) To investigate the applicability of the sample, mainly to investigate the four indicators of moisture, total ash, acid-insoluble ash and leaching, according to the second method of 0832 in the four general rules of the 2015 edition of the Chinese Pharmacopoeia, the total ash determination method under item 2302, Determination of acid insoluble ash under item 2302 and determination under item 2201, the measurement results are shown in Table 2;

[0038] Table 2 List of determination results of sand cattle moisture, total ash, acid-insoluble ash and extracts ...

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Abstract

The invention discloses a thin-layer chromatography identification method for ant lions. The method comprises the following steps that: firstly, investigating the applicability of a sample, measuringthe total nitrogen contents of the ant lions, and carrying out the thin-layer chromatography identification of the cholesterol and the amino acid of the ant lions. The thin-layer chromatography identification method for ant lions has the advantages of convenience in operation, simple equipment, easiness in color development and high expansion speed, and the identification method for the ant lionsis normalized.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicine, in particular to a method for identifying sand cattle by thin-layer chromatography. Background technique [0002] The source of the sand cow in the "Standard List of Chinese Medicinal Materials" is the antlion, an antlion Myrme Leon formicarius L . , East China Antfly EuroLon sinicus (Navas) . or yellow-footed antfly Hagenomyia micans (MacLaehLan) dried larvae. Caught in autumn, scalded to death with boiling water, and dried. [0003] Anthridae is a kind of medicinal insect with the largest variety and widest distribution in Neuroptera, and it is distributed all over the country. Since ancient times, sand ox has been commonly used by the people to treat epilepsy, malaria, otitis media, convulsions with high fever in children, bruises and snake bites; in recent years, it has been found that using sand ox as the main ingredient can effectively treat v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 甘勇强张涛唐秀玲谢培德黄清泉燕霞
Owner 广西壮族自治区食品药品检验所
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