A kind of chlorine-substituted type II halogenated polyketide compound and its preparation method and application

Abstract

The invention discloses a monochloro substitution second type halogenated polyketone compound. The structural formula of the monochloro substitution second type halogenated polyketone compound is as shown in the description. The compound has antibacterial activity against Gram-positive bacteria, Gram-negative bacteria or fungi. More monochloro compounds are obtained by improving a preparation method, meanwhile the antibacterial activity of the compound is screened, and results show that the compound has spectrum antibacterial activity and has the antibacterial activity against the Gram-positive bacteria such as Staphylococcus aureus (MRSA) and drug-resistance bacteria, staphylococcus epidermidis and bacillus subtilis thereof; the compound has the antibacterial activity against the Gram-negative bacteria such as escherichia coli (ESBL) capable of producing hyper spectrum beta-lactamase, proteusbacillus vulgaris and bacterium burgeri; the compound has the antibacterial activity against the fungi such as Candida albicans.

Description

technical field

[0001] The invention relates to the technical field of microorganisms, in particular to a monochloro-substituted type II halogenated polyketide compound and its preparation method and application, in particular to a monochloro-substituted halogenated type II polyketide compound zunyimycin D and its application. Background technique

[0002] Natural products derived from Streptomyces often have good biological activity and drug potential, and are an important source of antibiotics. However, with the abuse of antibiotics, the emergence of many "super bacteria" has brought great challenges to the clinical treatment of diseases such as pathogenic bacterial infections, and some super bacteria have no cure. In view of the current increasingly severe antibacterial situation, increasing the research and development of antibiotics, exploring and developing new antibiotics has become a top priority, and it is also an effective way to solve the problem of antibiotic res...

Examples

Embodiment Construction

[0040] The present invention will be described in further detail below by means of specific embodiments:

[0041] 1. Activation of Streptomyces sp.FJS31-2 strain

[0042] Take out the strains preserved on the glycerol slant from the -80°C refrigerator, dig out the spores of Streptomyces sp. Place the culture for 3 days, pick a single colony and subculture to the third generation to scale up the culture.

[0043] 2. Enrichment of fermentation products

[0044]Select the improved GYD solid medium for fermentation and culture, add agar powder to the medium, and culture at 28°C for 17 days. The bacteria and the medium are crushed, and ethyl acetate is added to extract twice. The extracts are combined and concentrated under reduced pressure to obtain fermentation product.

[0045] 3. Purification and separation of fermentation products

[0046] (1) After the fermentation product is dissolved with acetone solvent, mix it with silica gel powder and use chloroform-acetone accordin...

technical field

[0001] The invention relates to the technical field of microorganisms, in particular to a monochloro-substituted type II halogenated polyketide compound and its preparation method and application, in particular to a monochloro-substituted halogenated type II polyketide compound zunyimycin D and its application. Background technique

[0002] Natural products derived from Streptomyces often have good biological activity and drug potential, and are an important source of antibiotics. However, with the abuse of antibiotics, the emergence of many "super bacteria" has brought great challenges to the clinical treatment of diseases such as pathogenic bacterial infections, and some super bacteria have no cure. In view of the current increasingly severe antibacterial situation, increasing the research and development of antibiotics, exploring and developing new antibiotics has become a top priority, and it is also an effective way to solve the problem of antibiotic res...

Examples

Embodiment Construction

[0040] The present invention will be described in further detail below by means of specific embodiments:

[0041] 1. Activation of Streptomyces sp.FJS31-2 strain

[0042] Take out the strains preserved on the glycerol slant from the -80°C refrigerator, dig out the spores of Streptomyces sp. Place the culture for 3 days, pick a single colony and subculture to the third generation to scale up the culture.

[0043] 2. Enrichment of fermentation products

[0044]Select the improved GYD solid medium for fermentation and culture, add agar powder to the medium, and culture at 28°C for 17 days. The bacteria and the medium are crushed, and ethyl acetate is added to extract twice. The extracts are combined and concentrated under reduced pressure to obtain fermentation product.

[0045] 3. Purification and separation of fermentation products

[0046] (1) After the fermentation product is dissolved with acetone solvent, mix it with silica gel powder and use chloroform-acetone accordin...

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