A method for simultaneous determination of 19 organochlorine pesticide residues in Panax notoginseng

A technology of organochlorine and pesticide residues, applied in the field of simultaneous determination of 19 kinds of organochlorine pesticide residues in Panax notoginseng. The effect of daily inspection and easy operation

Active Publication Date: 2022-03-25
TIANJIN TASLY PHARMA CO LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods are cumbersome to prepare samples, take a long time to test, use more toxic reagents such as dichloromethane, etc., column chromatography needs to be filled and processed, and require high testing personnel. These are not suitable for daily production testing.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for simultaneous determination of 19 organochlorine pesticide residues in Panax notoginseng
  • A method for simultaneous determination of 19 organochlorine pesticide residues in Panax notoginseng
  • A method for simultaneous determination of 19 organochlorine pesticide residues in Panax notoginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] A method for simultaneously measuring 19 kinds of organochlorine pesticide residues in Panax notoginseng, comprising the following steps:

[0062] (1) Preparation of the test solution:

[0063] Take 1 part of the medicinal powder, accurately weigh it, put it in a 50mL polypropylene plastic centrifuge tube, add 30 times the weight of the medicinal material in petroleum ether, soak it for 1 hour, filter it with suction, wash the residue with 15 times the weight of the medicinal material, and transfer it to a 100 mL circular In a bottom flask, it was rotary evaporated to near dryness in a water bath at 40°C. Reconstituted with 2 mL of n-hexane, transferred to a centrifuge tube, added 0.1 mL of concentrated sulfuric acid, shaken for 1 min, after the reaction was complete, centrifuged at 3000 r / min for 5 min, and the supernatant was taken and sent to GC for testing.

[0064] (2) Preparation of mixed reference solution:

[0065] Precisely measure 1 mL of each of 19 pesticid...

Embodiment 2

[0071] According to the method of Example 1, 30 batches of medicinal materials of Panax notoginseng originating in Yunnan were measured. The test results are shown in Table 4. Among them, the maximum residue of pentachloronitrobenzene is 0.32mg / kg, which exceeds the limit stipulated in the 2015 edition of the Chinese Pharmacopoeia, and the remaining pesticide residues are not exceeded.

[0072] Table 4 Test result table

[0073] Tab.4 Test results

[0074]

[0075]

[0076] (Note: "-" means not detected, "+" means detected, "++" means exceeded).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to view more

Abstract

The present invention relates to a detection method of 19 kinds of organochlorine pesticide residues in Panax notoginseng. The method comprises the following steps: (1) Preparation of the test solution: take medicinal material powder, add petroleum ether to soak, suction filter, and use Wash with petroleum ether several times, transfer to a round-bottomed flask, rotatively evaporate in a water bath at 25-45°C until nearly dry, redissolve with n-hexane, add concentrated sulfuric acid, shake fully, centrifuge after the reaction is complete, and take the supernatant for later use (2) Preparation of mixed reference substance solution: get 19 kinds of pesticide standard items and put in volumetric flask and add n-hexane and dilute to the scale to get each reference substance stock solution, take by weighing respectively reference substance stock solution and put in volumetric flask, use normal hexane Dilute to the mark to get the mixed reference substance solution; (3) Determination method: take the test solution, mix the reference substance solution and inject it into the gas chromatograph for determination.

Description

Technical field: [0001] The invention relates to a method for detecting pesticide residues in traditional Chinese medicines, in particular to a method for simultaneously determining the residues of 19 kinds of organochlorine pesticides in Panax notoginseng. Background technique: [0002] The use of pesticides has brought great contributions to human life, while the abuse of pesticides has also caused certain harm to the human body. At present, with the continuous and in-depth development of standardized planting of Chinese medicinal materials, the problem of pesticide residues in medicinal materials has attracted more and more attention from scholars all over the world. Organochlorine pesticides were widely used in traditional Chinese medicine cultivation, but due to their long half-life, slow degradation and stable chemical properties, their use was banned in my country in the 1980s, but residual components can still be detected in water, soil and plants. . During the grow...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06
CPCG01N30/06
Inventor 李云飞佟玲李东翔段玺玉李爽
Owner TIANJIN TASLY PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap