Application of ripk4 as a target site in the preparation of drugs for the treatment of bladder cancer
A 1.RIPK4, bladder cancer technology, applied in the field of biomedicine, can solve problems such as unclear clinical and biological significance
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[0031] Below in conjunction with accompanying drawing and experimental data, the present invention is further explained and illustrated
[0032] 1. Vector, retrovirus infection and transfection
[0033]The vector pMSCV / RIPK4 overexpressing human RIPK4 was constructed by subcloning the human RIPK4 coding sequence amplified by PCR into the vector pMSCV (Clontech). I cloned four short hairpin RNA (shRNA) oligonucleotides to silence endogenous RIPK4 into the vector pSuper-retro-puro to generate pSuper-retro-RIPK4-shRNA(S). The NF-κB-p65 of the lentiviral ShRNA was constructed, and the ShRNA of the lentiviral VEGF-A was constructed. 48 hours after lentivirus infection, stable expression of RIPK4, RIPK4-ShRNA, NF-κB-p65-ShRNA, and VEGF-A-ShRNA was selected.
[0034] 2. RIPK4 is upregulated in BUC patients
[0035] In order to evaluate the expression model of RIPK4 in BUC, we analyzed the RIPK4 protein level in several BUC cell lines including BIU87, 5637, T24, EJ and RT4 cells, a...
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