Image-based white blood cell counting method and system

A white blood cell counting and white blood cell technology, which is applied in the field of white blood cell counting systems, can solve the problems of inability to obtain cell segmentation, low counting accuracy, and difficulty in distinguishing concave and convex points on the boundary.

CN108257124AActive Publication Date: 2018-07-06JIANGSU KONSUNG BIOMEDICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
Publication Date
2018-07-06

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Abstract

The invention relates to an image-based white blood cell counting method and system. The method includes the steps of collecting a blood sample image, removing image noise, conducting coarse segmentation on a cell region, obtaining the number of adherent cells, and obtaining the total number of white blood cells. According to the method, the segmentation accuracy of the white blood cells is higherby adopting a white blood cell automatic segmentation technique of an adaptive fuzzy clustering technology, the adherent cells with unapparent regions and chain code features can be quickly identified based on a three-dimensional surface adherent cell localization segmentation method, and the localization, counting and segmentation of the adherent cells are more accurate, so that the measurementof the number of the white blood cells is more accurate.
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Description

technical field

[0001] The invention relates to an image-based white blood cell counting method, and at the same time, the invention also relates to a white blood cell counting system. Background technique

[0002] As an indication of certain diseases, the number of white blood cells has important clinical significance for disease diagnosis. Physical method, physical-chemical method and image analysis method are the main methods of white blood cell differential counter at present. Although the automatic counters under these methods can quickly and accurately count white blood cells, they still have the characteristics of bulky equipment, high price, high professionalism in operation, and difficult maintenance, which are not suitable for primary medical and health institutions and mobile medical services. However, there are great limitations in the field of application. For the image-based leukocyte adhesion segmentation and counting method, it mainly focuses on the researc...

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Embodiment Construction

[0090] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0091] Please refer to figure 1 Referring to FIG. 7 , the present invention proposes an image-based white blood cell counting method for fast, accurate and convenient counting of the number of white blood cells in human peripheral blood. The specific steps are as follows.

[0092] Step S1: Image Acquisition

[0093] In order to improve the counting accuracy of white blood cells, before the method is implemented, trypan blue is used to stain the white blood cells in the blood sample to enhance the contrast of the cells, and then the red blood cells in the blood sample are lysed with the red blood cell lysate to reduce the interference of irrelevant cell impurities. The leukocyte staining and erythrocyte lysing methods in this step can also be reali...