An image-based white blood cell counting method and system

A white blood cell counting and white blood cell technology, which is applied in the field of white blood cell counting systems, can solve the problems of inability to distinguish the nucleus area, inability to identify adherent cells, and difficulty in distinguishing the concave and convex points on the boundary.

CN108257124BActive Publication Date: 2020-08-25JIANGSU KONSUNG BIOMEDICAL TECH
5 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-08-25

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to an image-based white blood cell counting method and system, wherein the method includes the steps of collecting blood sample images, removing image noise, roughly segmenting cell regions, obtaining the number of cohesive cells and obtaining the total number of white blood cells. Because the present invention adopts the white blood cell automatic segmentation technology of self-adaptive fuzzy clustering technology, the white blood cell segmentation accuracy is higher, and the adhesion cell location segmentation method based on the three-dimensional curved surface can quickly identify the adhesion cells with inconspicuous area and chain code characteristics, adhesion cells The location counting segmentation is more accurate, so that the number of white blood cells can be counted more accurately.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to an image-based white blood cell counting method, and at the same time, the invention also relates to a white blood cell counting system. Background technique

[0002] As an indication of certain diseases, the number of white blood cells has important clinical significance for disease diagnosis. Physical method, physical-chemical method and image analysis method are the main methods of white blood cell differential counter at present. Although the automatic counters under these methods can quickly and accurately count white blood cells, they still have the characteristics of bulky equipment, high price, high professionalism in operation, and difficult maintenance, which are not suitable for primary medical and health institutions and mobile medical services. However, there are great limitations in the field of application. For the image-based leukocyte adhesion segmentation and counting method, it mainly focuses on the researc...

Examples

Embodiment Construction

[0090] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0091] Please refer to figure 1 Referring to FIG. 7 , the present invention proposes an image-based white blood cell counting method for fast, accurate and convenient counting of the number of white blood cells in human peripheral blood. The specific steps are as follows.

[0092] Step S1: Image Acquisition

[0093] In order to improve the counting accuracy of white blood cells, before the method is implemented, trypan blue is used to stain the white blood cells in the blood sample to enhance the contrast of the cells, and then the red blood cells in the blood sample are lysed with the red blood cell lysate to reduce the interference of irrelevant cell impurities. The leukocyte staining and erythrocyte lysing methods in this step can also be reali...