Natural tubular small intestine acellular matrix membrane and preparation method thereof
An acellular matrix and small intestine technology, which is applied in the field of natural tubular small intestine acellular matrix membrane and its preparation, can solve the problem of backward preparation technology, late biological patch, reliability of suture edge, smooth inner wall of tube, uniformity of tube wall, tissue repair or Reconstruction effects and other issues to achieve good tubular uniformity
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[0031] The present invention firstly provides a kind of preparation method of natural tubular small intestine acellular matrix membrane, and this method comprises:
[0032] Step 1: immerse the small intestine cut from the animal in physiological saline for cleaning;
[0033] Step 2: Put the cleaned small intestine on the ring-shaped tubular stent, destroy the serosa layer with scissors, cut the serosa layer of the small intestine as much as possible, and then use a plexiglass plate to clean the serosa layer of the damaged small intestine. Scraping: After the scraping is clean, the small intestine is turned over, and then the mucosal layer, submucosa layer and muscular layer of the small intestine are scraped off to remove the small intestinal basal cells to obtain a single-layer tubular small intestinal acellular matrix membrane.
[0034] Step 3: Fix the single-layer tubular small intestinal acellular matrix membrane obtained in step 2 on the annular tubular scaffold, and then...
Embodiment 1
[0043] Example 1 Preparation of monolayer natural tubular ECM
[0044] Take 1 local white pig, weighing 50-100Kg, under continuous ether inhalation anesthesia, select the abdominal midline incision, strictly prepare the skin, sterilize, spread sterile towels, cut the small intestine of the pig with a 15 cm long section, and take it out Immediately after that, they were immersed in 4°C normal saline, and the small intestine was washed with normal saline. Put the cleaned small intestine on the annular tubular stent, destroy the serosa layer with scissors, cut the serosa layer of the small intestine as much as possible, and scrape off the damaged serosa layer with a plexiglass plate. After the scraping was completed, the small intestine was turned over, and then the mucous layer, submucosa and muscular layer of the small intestine were scraped off in the same way to remove the basal cells of the small intestine. The above layers were scraped off carefully to prevent cell residue...
Embodiment 2
[0048] Example 2 Preparation of double-layer natural tubular ECM
[0049] Two adjacent intestinal tubes of the same small white pig were selected, with the same diameter and length, and two single-layer tubular small intestinal acellular matrix membranes were prepared according to the method in Example 1.
[0050] Fix the above-mentioned single-layer tubular small intestine acellular matrix membrane on the annular tubular support, and then connect one end of another single-layer tubular small intestinal acellular matrix membrane with the same specification (same length and diameter) prepared by the same method to the single-layer tubular small intestinal acellular matrix membrane on the support. One end of the tubular small intestine acellular matrix membrane is fixed, and then the other end of the tubular small intestinal acellular matrix membrane fixed on the support is turned over, and pulled along one end of the support to the other end, maintaining the same tension, and on...
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