Natural tubular small intestine acellular matrix membrane and preparation method thereof

An acellular matrix and small intestine technology, which is applied in the field of natural tubular small intestine acellular matrix membrane and its preparation, can solve the problem of backward preparation technology, late biological patch, reliability of suture edge, smooth inner wall of tube, uniformity of tube wall, tissue repair or Reconstruction effects and other issues to achieve good tubular uniformity

Inactive Publication Date: 2018-07-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reliability of the sutured edge, the smoothness of the inner wall of the tube, and the uniformity of the tube wall will affect the repair or reconstruction of the tissue of the tubular ECM biological patch formed by sewing or winding.
In my country, the research and application of ECM bio-patch started relatively late, and the preparation technology is relatively backward, especially for the preparation and application of natural tubular ECM bio-patch is still in the blank

Method used

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  • Natural tubular small intestine acellular matrix membrane and preparation method thereof
  • Natural tubular small intestine acellular matrix membrane and preparation method thereof
  • Natural tubular small intestine acellular matrix membrane and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0031] The present invention firstly provides a kind of preparation method of natural tubular small intestine acellular matrix membrane, and this method comprises:

[0032] Step 1: immerse the small intestine cut from the animal in physiological saline for cleaning;

[0033] Step 2: Put the cleaned small intestine on the ring-shaped tubular stent, destroy the serosa layer with scissors, cut the serosa layer of the small intestine as much as possible, and then use a plexiglass plate to clean the serosa layer of the damaged small intestine. Scraping: After the scraping is clean, the small intestine is turned over, and then the mucosal layer, submucosa layer and muscular layer of the small intestine are scraped off to remove the small intestinal basal cells to obtain a single-layer tubular small intestinal acellular matrix membrane.

[0034] Step 3: Fix the single-layer tubular small intestinal acellular matrix membrane obtained in step 2 on the annular tubular scaffold, and then...

Embodiment 1

[0043] Example 1 Preparation of monolayer natural tubular ECM

[0044] Take 1 local white pig, weighing 50-100Kg, under continuous ether inhalation anesthesia, select the abdominal midline incision, strictly prepare the skin, sterilize, spread sterile towels, cut the small intestine of the pig with a 15 cm long section, and take it out Immediately after that, they were immersed in 4°C normal saline, and the small intestine was washed with normal saline. Put the cleaned small intestine on the annular tubular stent, destroy the serosa layer with scissors, cut the serosa layer of the small intestine as much as possible, and scrape off the damaged serosa layer with a plexiglass plate. After the scraping was completed, the small intestine was turned over, and then the mucous layer, submucosa and muscular layer of the small intestine were scraped off in the same way to remove the basal cells of the small intestine. The above layers were scraped off carefully to prevent cell residue...

Embodiment 2

[0048] Example 2 Preparation of double-layer natural tubular ECM

[0049] Two adjacent intestinal tubes of the same small white pig were selected, with the same diameter and length, and two single-layer tubular small intestinal acellular matrix membranes were prepared according to the method in Example 1.

[0050] Fix the above-mentioned single-layer tubular small intestine acellular matrix membrane on the annular tubular support, and then connect one end of another single-layer tubular small intestinal acellular matrix membrane with the same specification (same length and diameter) prepared by the same method to the single-layer tubular small intestinal acellular matrix membrane on the support. One end of the tubular small intestine acellular matrix membrane is fixed, and then the other end of the tubular small intestinal acellular matrix membrane fixed on the support is turned over, and pulled along one end of the support to the other end, maintaining the same tension, and on...

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Abstract

The invention provides a natural tubular small intestine acellular matrix membrane and a preparation method thereof and belongs to the technical field of medical biomaterials. The method comprises washing small intestines taken from animals, sleeving an annular tubular support with the cleaned small intestines, breaking the serosa layer with scissors, scrapping the damaged small intestine serosa layer through an organic glass plate, turning over the small intestines, scrapping to remove the mucosal layer, submucosa and muscle layer of the small intestine, removing small intestine basal cells to obtain a single-layer tubular small intestine acellular matrix membrane, stacking the tubular small intestine acellular matrix membranes with the same specification on the support, carrying out pressing bonding to obtain a multi-layer tubular small intestine acellular matrix membrane, carrying out freeze-drying on the single-layer and multi-layer tubular small intestine acellular matrix membranes and carrying out sterilization preservation to obtain natural tubular small intestine acellular matrix membranes with different thickness. The preparation method has simple processes. The small intestine acellular matrix membrane is naturally tubular.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a natural tubular small intestine acellular matrix membrane and a preparation method thereof. Background technique [0002] Small intestinal decellularized matrix membrane (ECM) biopatch refers to the three-dimensional framework of the extracellular matrix (ECM) that is taken from the same or heterogeneous tissue, and the various cells contained in the tissue are removed through decellularization. Structure and materials used to repair body tissues. When the ECM biological patch is used for tissue and organ repair or reconstruction, the ECM can act as a "mold". The ECM biological patch transmits biological information to the host's ECM receptors, induces and stimulates the corresponding tissue and organ cells to attach to its surface through integrins, laminin receptors, etc., migrates from the edge to the center, and induces cells to follow the "mold" ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 韩春山冯永刚薛锦儒张乐宁孙宏斌王图慧冯敏崔建华张丽萍
Owner JILIN UNIV
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