Method for preparing natural tubular small intestine acellular matrix membrane

Abstract

The invention provides a method for preparing a natural tubular small intestine acellular matrix membrane and belongs to the technical field of medical biological materials. The method comprises the following steps: cleaning small intestines cut from animals; adding the cleaned small intestines into a closed container, rapidly freezing in liquid nitrogen at the temperature of 80 DEG C below zero,taking out within half an hour, unfreezing, sleeving on an annular tubular scaffold, destroying the serosa by scissors, cutting off the serosa of the small intestines, scraping the destroyed serosa ofthe small intestines by organic glass, turning over the small intestines, scraping the mucous layer, submucosa and muscular layer of the small intestines, and removing the basal cells of the small intestines, thereby obtaining the single-layer tubular small intestine acellular matrix membrane; and performing freeze-dried seal and sterilizing preservation on the obtained single-layer and multilayer tubular small intestine acellular matrix membranes, thereby obtaining the natural tubular small intestine acellular matrix membranes of different thicknesses. The preparation method disclosed by theinvention is simple, and the prepared small intestine acellular matrix membrane is natural tubular.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a method for preparing a natural tubular small intestine acellular matrix membrane. Background technique [0002] Small intestinal decellularized matrix membrane (ECM) biopatch refers to tissue taken from the same or heterogeneous tissue, which is decellularized to remove various cells contained in the tissue while retaining the three-dimensional extracellular matrix (ECM) intact. Frame structure and material used to repair body tissue. When the ECM biological patch is used for tissue and organ repair or reconstruction, the ECM can act as a "mold". The ECM biological patch transmits biological information to the host's ECM receptors, induces and stimulates the corresponding tissue and organ cells to attach to its surface through integrins, laminin receptors, etc., migrates from the edge to the center, and induces cells to follow the "mold" Differentiate...

AI Technical Summary

Problems solved by technology

However, the reliability of the sutured edge, the smoothness of the inner wall of the tube, and the uniformity of the tube wall will affect the repair or reconstruction of the tissue of the tubular ECM biological patch formed by sewing or winding.

In my country, the research and application of ECM bio-patch started relatively late, and the preparation technology is relatively backward, especially for the preparation and application of natural tubular ECM bio-patch is still in the blank

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