Tartary buckwheat seed germination drought-resisting stress device and stress method thereof

A technology of tartary buckwheat and seeds, applied in seed and rhizome treatment, germination equipment, application, etc., can solve the problems of unsuitable tartary buckwheat seed germination and drought resistance stress, inaccurate test results of tartary buckwheat samples, and affecting the normal growth of tartary buckwheat, etc. Achieve the effects of saving experimental costs, avoiding changes in solution concentration, and reducing secondary damage

Pending Publication Date: 2018-07-27
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tartary buckwheat seedlings are relatively fragile, and wrapping them with germination paper rolls will affect the normal growth of tartary buckwheat, resulting in inaccurate tartary buckwheat samples and test results
[0006] The above methods are not suitable for the germination of tartary buckwheat seeds to resist drought stress. At the same time, the drought resistance methods at the seedling stage have uneven emergence, low efficiency, poor sample repeatability, secondary damage to the sample, and inaccurate results.

Method used

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  • Tartary buckwheat seed germination drought-resisting stress device and stress method thereof
  • Tartary buckwheat seed germination drought-resisting stress device and stress method thereof
  • Tartary buckwheat seed germination drought-resisting stress device and stress method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Screening of tartary buckwheat varieties with high drought resistance.

[0036] Choose 2 tartary buckwheat seed germination anti-drought stress devices of the present invention of the same specification. The plump tartary buckwheat seeds of 2 varieties were selected, washed and sterilized, and placed in the drought stress device for germination of tartary buckwheat seeds added with sterile water filter paper, with 3-10 replicates for each variety, and cultured in the dark at 20°C for 10 days. The control group continued to culture with sterile water; the drought treatment group picked up the tartary buckwheat seedlings with tweezers, took out the sterile water filter paper and replaced it with 25% PEG6000 solution filter paper, put the tartary buckwheat seedlings on the filter paper again, and kept them at 20°C. Cultured in dark conditions.

[0037] Add 25% PEG6000 solution on the filter paper and cultivate it for 2 days, 4 days, and 6 days to take samples, ...

Embodiment 2

[0046] Embodiment 2: Improve the ability of tartary buckwheat to adapt to drought.

[0047] Choose 2 tartary buckwheat seed germination anti-drought stress devices of the present invention of the same specification. Pick plump tartary buckwheat grains of a variety, wash and disinfect them, and place them in two tartary buckwheat seed germination drought-resistant stress devices added with sterile water filter paper, a control group and a drought treatment group, with 3-10 repetitions for each treatment, Incubate in the dark at 20°C for 5 days. The drought treatment group was replaced with filter paper wetted with 25% PEG6000 solution for 2 days, and then cultured with filter paper wetted with water for 5 days, and then continued with filter paper wetted with 25% PEG6000 solution; The filter paper was cultured for 7 days, and then the filter paper wetted with 25% PEG6000 solution was used to continue the culture.

[0048] After 25% PEG6000 drought treatment, samples were take...

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PUM

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Abstract

The invention provides a tartary buckwheat seed germination drought-resisting stress device and a stress method thereof. The tartary buckwheat seed germination drought-resisting stress device comprises a main box, a cover plate and a bottom box. The main box is internally provided with multiple hollow cubes, and the bottom of each hollow cube is provided with a small hole. The small hole is provided with filter paper. The cover plate is installed on the main box. The main box is installed on the bottom box. The bottom box is internally provided with water. The tartary buckwheat seed germination drought-resisting stress device is capable of enabling tartary buckwheat seeds to complete two states of the germination and the drought-resisting stress treatment in one device, the device is simple in structure, and has the remarkable preventive effect to weak seedlings of the tartary buckwheat. In a germination aspect, suitable amount of water can be provided to the tartary buckwheat for thegermination and the growth, and the emergence of seedlings is tidy; and in a drought-resisting stress aspect, the secondary injure to the seedlings can be reduced in a device movement process, the change of the solution concentration caused by the water desorption is avoided, a lot of samples can be produced in the same box and the repeatability and the accuracy of the samples are guaranteed. Thewhole method is short in used time, high in efficiency, accurate in result, and low in cost.

Description

technical field [0001] The invention relates to the field of crop seed germination drought stress resistance, in particular to a tartary buckwheat seed germination drought stress resistance device and a stress method thereof. Background technique [0002] Drought has become a global problem with rapid population growth and increasing water scarcity. Drought not only caused huge losses in agricultural production, but also exacerbated land desertification and deterioration of ecological environment. Drought has become a huge challenge for agricultural production. Therefore, improving the drought-resistant adaptability of crops, screening drought-resistant varieties, understanding the physiological and molecular mechanisms of crop drought-resistance, and effectively improving water use efficiency have become important research topics. [0003] Tartary buckwheat, as the "king of five grains" and "three-falling food", has been in increasing demand in recent years. The main produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/00
CPCA01C1/00A01C1/02
Inventor 万燕欧阳建勇赵钢向达兵邬晓勇谭茂玲吴琪白雪马成瑞乐梨庆
Owner CHENGDU UNIV
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