Tartary buckwheat seed germination drought-resisting stress device and stress method thereof
A technology of tartary buckwheat and seeds, applied in seed and rhizome treatment, germination equipment, application, etc., can solve the problems of unsuitable tartary buckwheat seed germination and drought resistance stress, inaccurate test results of tartary buckwheat samples, and affecting the normal growth of tartary buckwheat, etc. Achieve the effects of saving experimental costs, avoiding changes in solution concentration, and reducing secondary damage
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Embodiment 1
[0035] Example 1: Screening of tartary buckwheat varieties with high drought resistance.
[0036] Choose 2 tartary buckwheat seed germination anti-drought stress devices of the present invention of the same specification. The plump tartary buckwheat seeds of 2 varieties were selected, washed and sterilized, and placed in the drought stress device for germination of tartary buckwheat seeds added with sterile water filter paper, with 3-10 replicates for each variety, and cultured in the dark at 20°C for 10 days. The control group continued to culture with sterile water; the drought treatment group picked up the tartary buckwheat seedlings with tweezers, took out the sterile water filter paper and replaced it with 25% PEG6000 solution filter paper, put the tartary buckwheat seedlings on the filter paper again, and kept them at 20°C. Cultured in dark conditions.
[0037] Add 25% PEG6000 solution on the filter paper and cultivate it for 2 days, 4 days, and 6 days to take samples, ...
Embodiment 2
[0046] Embodiment 2: Improve the ability of tartary buckwheat to adapt to drought.
[0047] Choose 2 tartary buckwheat seed germination anti-drought stress devices of the present invention of the same specification. Pick plump tartary buckwheat grains of a variety, wash and disinfect them, and place them in two tartary buckwheat seed germination drought-resistant stress devices added with sterile water filter paper, a control group and a drought treatment group, with 3-10 repetitions for each treatment, Incubate in the dark at 20°C for 5 days. The drought treatment group was replaced with filter paper wetted with 25% PEG6000 solution for 2 days, and then cultured with filter paper wetted with water for 5 days, and then continued with filter paper wetted with 25% PEG6000 solution; The filter paper was cultured for 7 days, and then the filter paper wetted with 25% PEG6000 solution was used to continue the culture.
[0048] After 25% PEG6000 drought treatment, samples were take...
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