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Special reagent for rapid microwave pathological tissue treatment and preparation method thereof

A technology of tissue processing and microwave, which is applied in the field of special reagents for rapid microwave pathological tissue processing and its preparation, can solve the problems of providing diagnosis and treatment basis, and not being able to timely patients, etc., achieves remarkable effects of fixation and dehydration, improves diagnostic efficiency, and has a remarkable effect Effect

Active Publication Date: 2018-07-31
李西启
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional pathological film production requires more than ten steps such as fixation, dehydration, and transparency. The whole process lasts for 16-22 hours, resulting in 3-5 working days for routine pathological diagnosis, which cannot provide timely diagnosis and treatment for patients. Therefore, the fast and efficient pathological section technology is very important

Method used

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  • Special reagent for rapid microwave pathological tissue treatment and preparation method thereof
  • Special reagent for rapid microwave pathological tissue treatment and preparation method thereof

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Effect test

Embodiment 1

[0028] A special reagent for rapid microwave pathological tissue treatment, including liquid A, liquid B and liquid C. The liquid A is mainly prepared from the following volume parts of raw materials: 70 parts of alcohol compounds, 10 parts of fatty acids, and 1 part of carbolic acid The B liquid is mainly prepared by the following volume parts of raw materials: 85 parts of alcohol compounds and 0.1 part of carbolic acid; the C liquid is mainly composed of the following volume parts of raw materials: 30 long-chain hydrocarbons Parts, 10 parts of isoparaffins, 10 parts of higher alcohols, 10 parts of methyl salicylate. The alcohol compound in liquid A is ethanol, the fatty acid in liquid A is glacial acetic acid; the alcohol compound in liquid B is isopropanol; the long-chain hydrocarbon in liquid C is n-heptane , The isoparaffin in liquid C is isododecane, and the higher alcohol in liquid C is n-hexanol.

[0029] The steps of the preparation method of a special reagent for rapid...

Embodiment 2

[0044] A special reagent for rapid microwave pathological tissue treatment, including liquid A, liquid B and liquid C. The liquid A is mainly prepared by the following volume parts of raw materials: 80 parts of alcohol compounds, 20 parts of fatty acids, and 5 parts of carbolic acid The B liquid is mainly prepared by the following volume parts of raw materials: 90 parts of alcohol compounds and 2 parts of carbolic acid; the C liquid is mainly composed of the following volume parts of raw materials: long-chain hydrocarbons 45 Parts, 25 parts of isoparaffins, 15 parts of higher alcohols, 15 parts of methyl salicylate. The alcohol compound in the liquid A is prepared by ethanol and isopropanol in a volume ratio of 3:2, and the fatty acid in liquid A is prepared by glacial acetic acid and formic acid in a volume ratio of 3:5; The alcohol compounds in the B solution are ethanol, isopropanol and tert-butanol in a volume ratio of 2:5:7; the long-chain hydrocarbons in the C solution ar...

Embodiment 3

[0060] A special reagent for rapid microwave pathological tissue processing, including liquid A, liquid B and liquid C. The liquid A is mainly prepared from the following volume parts of raw materials: 75 parts of alcohol compounds, 15 parts of fatty acids, and 3 parts of carbolic acid The B liquid is mainly prepared by the following volume parts of raw materials: 90 parts of alcohol compounds and 1.5 parts of carbolic acid; the C liquid is mainly composed of the following volume parts of raw materials: 40 long-chain hydrocarbons Parts, 20 parts of isoparaffins, 12 parts of higher alcohols, 14 parts of methyl salicylate. The alcohol compound in liquid A is isopropanol, the fatty acid in liquid A is formic acid; the alcohol compound in liquid B is tert-butanol; the long-chain hydrocarbon in liquid C is normal Heptane and n-hexane are prepared in a volume ratio of 1:3. The isoparaffins in liquid C are isododecane and isohexadecane in a volume ratio of 3:4. The higher alcohol is ...

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Abstract

The invention relates to the field of clinical pathological diagnosis reagents, in particular to a special reagent for rapid microwave pathological tissue treatment and a preparation method thereof. The special reagent for rapid microwave pathological tissue treatment comprises an A liquid, a B liquid and a C liquid, the A liquid is prepared from, by volume, 70-90 parts of alcohol compound, 10-30parts of fatty acid and 1-10 parts of phenol; the B liquid is prepared from, by volume, 85-95 parts of alcohol compound and 0.1-5 parts of phenol; the C liquid is prepared from, by volume, 30-60 partsof long-chain hydrocarbon, 10-40 parts of isoparaffin, 10-20 parts of higher alcohols and 10-20 parts of methyl salicylate. According to the special reagent for rapid microwave pathological tissue treatment and the preparation method thereof, the collocation is reasonable, the preparation process is simple and convenient, the pathological diagnosis time is shortened to 1-3 hours, the special reagent for rapid microwave pathological tissue treatment does not contain any volatile substances harmful to the human body, and thereby ensuring the health of medical staffs and the environmental protection.

Description

Technical field [0001] The invention relates to the field of clinical pathological diagnosis reagents, in particular to a special reagent for rapid microwave pathological tissue processing and a preparation method thereof. Background technique [0002] According to reports, the number of new cancer patients in China each year accounts for more than 20% of the global number. Early diagnosis and early treatment of cancer patients is the key to improving the survival rate of patients. At present, the early diagnosis of cancer mainly depends on pathological slices for judgment. However, traditional pathology filming requires more than ten steps such as fixation, dehydration, and transparency. The entire process lasts 16-22 hours. As a result, routine pathological diagnosis takes 3-5 working days to complete, and diagnosis and treatment cannot be provided in time. Therefore, the rapid and efficient pathological section technology is very important. In addition, most of the fixatives...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/2813G01N1/30G01N2001/305G01N2001/307
Inventor 李锴男赫军李西启徐廷香吴品佐王海洋徐大鹏魏芳芳
Owner 李西启
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