Primer, probe and kit for BIM gene polymorphism detection

Abstract

The invention discloses a BIM gene polymorphism primer based on a TaqMan fluorescence probe, a probe and a detection kit. The kit comprises the primer and the probe aiming at BIM-Exon2-3 and / or BIM-Exon2-4. The primer aiming at the BIM-Exon2-3 is as shown in SEQ ID NO. 1 and SEQ ID NO. 2, and the probe aiming at the BIM-Exon2-3 is as shown in SEQ ID NO. 11; and the primer aiming at the BIM-Exon2-4is as shown in SEQ ID NO. 3 and SEQ ID NO. 4, and the probe aiming at the BIM-Exon2-4 is as shown in SEQ ID NO. 12. The designed BIM gene specific TaqMan probe has the sequence specificity, and is only combined to a complementation area, and fluorescence signals correspond to amplified copy numbers one by one, so the specificity is strong, and the sensitivity is high. Even if the target gene content in a sample is excessive low, the target gene can be detected, the sensitivity of the sample BIM gene deletion polymorphism detection is improved.

Description

Technical field [0001] The invention belongs to the field of gene detection, and specifically relates to primers, probes and kits for detecting BIM gene polymorphism based on TaqMan fluorescent probes. Background technique [0002] Real-time fluorescent quantitative PCR technology effectively solves the limitation of traditional quantitative PCR (no probe or real-time fluorescent dye incorporation, etc.) that can only take out PCR products for separation and end-point detection, and realizes that fluorescence is detected once in each cycle The signal intensity is recorded in the computer software, and the quantitative result is obtained according to the standard curve by calculating the Ct value (the number of cycles of reaching the threshold signal) of each sample. Fluorescent substances used in real-time fluorescent quantitative PCR can be divided into two types: fluorescent probes and fluorescent dyes. At present, the most commonly used real-time PCR technology is SYBR Green ...

AI Technical Summary

Problems solved by technology

In the presence of BIM gene deletion polymorphism, in the process of cell apoptosis induced by antitumor drugs, the expression of BIMγ protein that does not contain BH3 homology domain is up-regulated, while the main protein Bim EL that plays a pro-apoptotic role is decreased. As a result, a large number of non-functional BIM proteins cannot bind to anti-apoptotic proteins through the BH3 domain, neutralize the anti-apoptotic effect, and fail to activate Bax and Bak to promote endogenous apoptotic pathways, resulting in the phenomenon of endogenous drug resistance

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