A novel mercury-free and environmentally friendly hematoxylin dye solution and its preparation method

A technology of hematoxylin and dye solution, applied in the field of pathology experiments, can solve the problems of precipitation, dark nuclear staining, and unclear levels of nuclear and cytoplasmic staining
CN108531540BActive Publication Date: 2021-01-01HARBIN GREEN SPECIMEN TECH DEV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
HARBIN GREEN SPECIMEN TECH DEV
Publication Date
2021-01-01

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Abstract

The invention discloses novel mercury-free environmentally-friendly hematoxylin dyeing liquid and a preparation method thereof and belongs to the technical field of a pathological experiment. The dyeing liquid is mainly prepared by the following raw materials: hematoxylin, a mordant reagent, an oxidizing agent, acid, ethylene diamine tetraacetic acid disodium salt, iso-tridecanol polyoxyethylene ether, glycerin and water; a Mordant is any one of aluminum potassium sulfate dodecahydrate, aluminum sulfate, ferric trichloride and copper sulfate; the oxidizing agent is any one of sodium iodate andpotassium permanganate; the acid is any one of acetic acid, hydrochloric acid and citric acid. The novel mercury-free environmentally-friendly hematoxylin dyeing liquid disclosed by the invention successfully solves the problem of HE dyeing troubled in the medical pathological diagnosis field for a long term, for example, hematoxylin dyeing liquid is poor in stability and short in shelf life, isprovided with a metal film, has dye residues, foams and is unclear in corpuscular contrast, unclear in level and dark in nuclear dyeing color and the like.
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Description

technical field

[0001] The invention relates to a novel mercury-free environment-friendly hematoxylin dye solution and a preparation method thereof, belonging to the technical field of pathological experiments. Background technique

[0002] Hematoxylin stain is a routine nuclear stain in pathology laboratories, and its formulations are also varied. The main differences between the common hematoxylin dyes on the market are: 1. The oxidants are different. There are long-term natural oxidation, rapid oxidation using mercury oxide and potassium permanganate oxidant, and slow oxidation using sodium iodate oxidant; Second, the difference of mordant. Common mordants are aluminum sulfate, ferric chloride, potassium aluminum sulfate dodecahydrate, copper sulfate, etc.; 3. The acids used are different. Commonly used acids are acetic acid, hydrochloric acid, citric acid, etc.

[0003] But in general, each formula of hematoxylin dye solution has common problems: 1. Excessive oxidatio...

Claims

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