Microfluidic chip for separation and detection of whole blood samples and its detection method

Abstract

The present invention provides a microfluidic chip for the separation and detection of whole blood samples, comprising a chip body, the chip body is provided with a sample flow channel; the sample flow channel includes a sample injection area and a settling area connected in sequence , a mixing zone, a detection zone and a waste liquid zone; the settling zone includes a sampling portion and a settling portion, and the ratio of the maximum width of the settling portion to the maximum width of the sampling portion is 2-10; the The settling part is a structure with narrow sides and wide middle; the front and rear side walls at both ends of the settling part are inclined planes, and the extension lines of the front and rear side walls intersect to form an included angle; the middle part of the settling part The front and rear sidewalls are parallel planes parallel to each other. The microfluidic chip can combine the separation and detection of plasma in whole blood, does not require complex whole blood sample pretreatment process, and can quickly and quantitatively detect single or multiple proteins or other indicators in whole blood.

Description

technical field [0001] The invention relates to the technical field of fluid sample detection, in particular to a microfluidic chip for separation and detection of whole blood samples and a detection method thereof. Background technique [0002] Analysis of blood components and their content is the most basic item in modern medical testing. Whole blood is composed of liquid plasma and blood cells. Because blood cells or hemoglobin cause great interference to spectral analysis, it is usually necessary to separate plasma from blood samples and then use it for biochemical or immunodiagnostic analysis. At present, the most commonly used plasma separation methods clinically are centrifugation and filtration, but both methods have certain shortcomings. The centrifugal method has bulky equipment and complicated operation; the filtration method has low separation efficiency and the sample is easily polluted. [0003] At present, POCT (Point Of Care Testing, instant detection) tech...

AI Technical Summary

Problems solved by technology

The centrifugal method is bulky and complicated to operate; the filtration method has low separation efficiency and the sample is easily polluted

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