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A kind of bacillus pumilus and its screening method, preparation method and application of bacterial agent

A technology of Bacillus pumilus and bacterial agents, applied in the field of microbiology, can solve the problems of few researches on the screening of multifunctional soil bacterial strains, and achieve significant application value, promote leaf growth, and promote plant growth.

Active Publication Date: 2022-02-08
WEIFANG TOBACCO MONOPOLY BUREAU
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing researches focus on the study of the functional flora of a single functional biological factor or different biological factors with the same function, and there are few studies on the screening of multifunctional soil bacterial strains

Method used

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  • A kind of bacillus pumilus and its screening method, preparation method and application of bacterial agent
  • A kind of bacillus pumilus and its screening method, preparation method and application of bacterial agent
  • A kind of bacillus pumilus and its screening method, preparation method and application of bacterial agent

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] 1. Acquisition of Bacillus pumilus

[0018] Tobacco field soil was collected from Zhucheng City and Changle County, Shandong Province, and a total of 31 bacterial strains were isolated and purified by the soil dilution method for subsequent experiments.

[0019] 2. Strain screening

[0020] Bacteria isolated and purified from tobacco field soil were inoculated into potassium-decomposing primary screening medium. Cultivate at 28°C for 120 hours, observe and record the colonies with transparent circles, and measure the diameter of the transparent circle (D) and the diameter of the colony (d).

[0021] The strain Zcg obtained by the primary screening plate method for potassium decomposing has good inorganic phosphorus and potassium decomposing effects.

[0022] Table 1: Statistical table of the potassium-dissolving effect of the strain Zcg

[0023]

[0024] 3. Identification of strain Zcg

[0025] 1. Morphological identification of strain Zcg

[0026] See figure 1...

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Abstract

The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus pumilus and its application. The preservation number of the strain is CGMCC No.13448. The Bacillus pumilus is named Bacillus pumilus BP16, and its 16S rDNA sequence is as shown in SEQ ID No: As shown in 1, the Bacillus pumilus has a good potassium-dissolving effect, is safe to humans, livestock and crops, and is environmentally friendly.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacillus pumilus, a screening method thereof, and a preparation method and application of a bacterial agent. Background technique [0002] Soil microorganisms and plant roots form a stable dynamic system. In this system, they interact and influence each other. Plant roots provide nutrients for soil microorganisms, and soil microorganisms can improve soil fertility and promote plant growth and development. Therefore, soil, plants It interacts with microorganisms in agricultural production and has a close relationship. Potassium is an essential macronutrient element for crop growth and development, and it is of great significance to study the availability of potassium in soil to improve soil fertility. Screening and studying potassium-dissolving bacteria in different soils is of great significance for developing microbial strain resources, improving soil fertility, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/07
CPCC12N1/20C05F11/08C12R2001/07C12N1/205
Inventor 杨德廉崔志军高传军张英华王先伟臧传江郭全伟赵湘江刘宏高政绪王德权席元肖杨少杰刘振宇孔维建
Owner WEIFANG TOBACCO MONOPOLY BUREAU
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