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Method for separating and purifying polysaccharides of dendrobium officinale by aqueous two-phase extraction

A technology for separation and purification of Dendrobium officinale polysaccharide, which is applied in the field of plant active ingredient extraction to achieve the effects of improving purity, scavenging hydroxyl radicals, and significant antioxidant capacity.

Inactive Publication Date: 2018-11-20
SHANXI UNIV
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Problems solved by technology

[0005] The present invention provides a method for separating and purifying polysaccharides from Dendrobium officinale by two-phase extraction in order to solve the problem of using an organic solvent with potential safety hazards as an extractant to purify proteins mixed in polysaccharides in the existing polysaccharide extraction of Dendrobium officinale

Method used

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  • Method for separating and purifying polysaccharides of dendrobium officinale by aqueous two-phase extraction
  • Method for separating and purifying polysaccharides of dendrobium officinale by aqueous two-phase extraction
  • Method for separating and purifying polysaccharides of dendrobium officinale by aqueous two-phase extraction

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Embodiment 1

[0013] Example 1: A method for separating and purifying Dendrobium officinale polysaccharides by aqueous two-phase extraction. Dendrobium officinale stems were dried at 30-41°C and pulverized to 50-100 mesh; accurately weighed 0.3g of Dendrobium officinale powder according to 1:50 Add 15mL of activated water treated with "Fuji no Shuijing" to the liquid ratio; keep the solution temperature at 35-41℃, and perform 2 ultrasonic treatments at 250W for 20-30 minutes each; combine the 2 extracts and dilute the volume to 100 mL In a volumetric flask, shake well, accurately measure 2 mL of the extract and place it in a 10 mL centrifuge tube, accurately add 8 mL of 95% ethanol, shake well, refrigerate at 4 ℃ for 12 h, take it out and centrifuge at 4000 r / min for 20 min, discard The supernatant is left with the precipitate, and the precipitate is washed twice with 80% ethanol. Add 8 mL of 80% ethanol each time. Heat the precipitate to dissolve it in activated water, and dilute to a 50 mL ...

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Abstract

The invention belongs to the technical field of plant active ingredient extraction, and provides a method for separating and purifying polysaccharides of dendrobium officinale by aqueous two-phase extraction in order to solve the problem of using an organic solvent having a safety hazard as an extractant to purify proteins mixed in the polysaccharides in extraction of the polysaccharides from thedendrobium officinale. Stalks of the dendrobium officinale are dried and pulverized; powder of the dendrobium officinale is dissolved in active water according to a ratio of material to liquid 1: 50,and a solution is treated twice by ultrasonic waves; leaching liquors of two-time ultrasound are combined; the leaching liquors are precipitated with ethanol; and obtained precipitation is heated anddissolved by ethanol with the concentration 80%, and the proteins mixed in crude polysaccharides are removed with a PEG / ammonium sulfate mixed solution. Usage of toxic chloroform is avoided to purifythe crude polysaccharides, and the purity reaches 90-95%. The extraction rate is 177-187% of the extraction rate of a conventional hot water extraction method and an ultrasonic extraction method. Thepolysaccharides obtained by extraction has obvious capability to remove DPPH free radicals and hydroxyl radicals, and has significant antioxidant capacity. The method provides reference for preparation methods of other plant polysaccharides.

Description

Technical field [0001] The invention belongs to the technical field of plant active ingredient extraction, and specifically relates to a method for separating and purifying Dendrobium officinale polysaccharides by aqueous two-phase extraction. Background technique [0002] Dendrobium is a genus of Dendrobium in Orchidaceae Dendrobium Perennial herb is a rare and precious Chinese medicinal material, known as "the fairy grass on earth". In the medical classic "Tao Zang", Dendrobium officinale is called the first of the nine immortal grasses in China, and the folks call it the life-saving fairy grass. Dendrobium officinale Dendrobium officinale, Commonly known as Tiepi Fengdou, it is a rare and precious medicinal material unique to our country. There are records of Dendrobium officinale being used as medicine in many ancient Chinese medical works such as Compendium of Materia Medica and Dictionary of Chinese Medicine. Modern biotechnology analysis shows that Dendrobium polysaccha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王景雪段梦妮
Owner SHANXI UNIV
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