Application of sodium hydrosulfide and melatonin combination serving as plant drought-resistant agent

A technology of sodium hydrosulfide and melatonin, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve the effects of increasing relative water content, increasing crop yield, and promoting stomatal closure

Inactive Publication Date: 2018-11-23
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Combination of two compounds (NaHS and melatonin) has never been reported

Method used

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  • Application of sodium hydrosulfide and melatonin combination serving as plant drought-resistant agent
  • Application of sodium hydrosulfide and melatonin combination serving as plant drought-resistant agent
  • Application of sodium hydrosulfide and melatonin combination serving as plant drought-resistant agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Arabidopsis thaliana seedlings in the same growth state were selected and exposed to light for 2 hours at 23°C. Weigh sodium hydrosulfide monohydrate (NaHS·H 2 O) Add distilled water to make 80 μmol L -1 The aqueous solution, the treatment solution is ready-to-use. Weigh melatonin powder and add 200 μl absolute ethanol to dissolve, then add distilled water to make 100 μmol L -1 of aqueous solution. At the same time, weigh PEG-8000 and add distilled water to make 0.15g·mL -1 of aqueous solution. A total of 7 treatment groups, of which the first three treatment groups were to put the plants into 7mL aqueous solution and spray 2mL of distilled water (control), 2mL of NaHS aqueous solution, and 2mL of melatonin aqueous solution, and the last four treatment groups were to put the plants into 7mL. .15g·mL -1 PEG-8000 aqueous solution was sprayed with distilled water 2mL, NaHS aqueous solution 2mL, melatonin aqueous solution 2mL, NaHS and melatonin aqueous solution 2mL ...

Embodiment 2

[0027] Arabidopsis thaliana seedlings in the same growth state were selected and exposed to light for 2 hours at 23°C. Weigh sodium hydrosulfide monohydrate (NaHS·H2O) and add distilled water to prepare 50 μmol·L -1 The aqueous solution, the treatment solution is ready-to-use. Weigh melatonin powder and add 200 μl absolute ethanol to dissolve, then add distilled water to make 50 μmol L -1 of aqueous solution. At the same time, weigh PEG-8000 and add distilled water to make 0.15g·mL -1 of aqueous solution. A total of 7 treatment groups, of which the first three treatment groups were to put the plants into 7mL aqueous solution and spray 2mL of distilled water (control), 2mL of NaHS aqueous solution, and 2mL of melatonin aqueous solution, and the last four treatment groups were to put the plants into 7mL. .15g·mL -1 PEG-8000 aqueous solution was sprayed with distilled water 2mL, NaHS aqueous solution 2mL, melatonin aqueous solution 2mL, NaHS and melatonin aqueous solution 2...

Embodiment 3

[0029] Prepare PEG, NaHS and melatonin aqueous solutions according to the above method, place them in a petri dish individually or jointly, and the final concentrations of the aqueous solutions are 0.15g·mL -1 PEG, 30 μmol L -1 NaHS and 60 μmol L -1 melatonin. Select Arabidopsis thaliana seedlings in the same growth state, weigh M1, put them into the corresponding treatment solution, weigh M2 after 6 hours, put the plants in 110°C, kill them for 30 minutes, and then dry them overnight at 55°C until the quality does not change. Weigh M3 and calculate the relative water content.

[0030] The weighing data of the seven groups of samples before and after treatment are shown in Table 1.

[0031] Table 1 Leaf mass after different treatments

[0032]

[0033] Biostatistical results show that the combined treatment of NaHS aqueous solution and melatonin can significantly increase the relative water content of plants under drought treatment (see attached image 3 ).

[0034] ...

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Abstract

The invention discloses application of sodium hydrosulfide and melatonin combination serving as a plant drought-resistant agent. According to the application, NaHS and melatonin are prepared into water solutions for utilization respectively, wherein the concentration of a NaHS water solution is 5 to 80mumol*L<-1> and the concentration of a melatonin water solution is 50 to 100mumol*L<-1>; uniformly applying the prepared water solutions on surface of plant leaves in sequence so as to extremely remarkably promote the closing of air pores and improve the relative water content of the leaves, andfurther alleviate damages, caused by drought stress, to plants. The application has the advantages of low cost, safety and no toxicity, simplicity in operation and remarkable effect; in order to solvethe drought-resistant problem in agricultural production, the invention provides a practical and effective method.

Description

technical field [0001] The present invention relates to the application of sodium hydrosulfide and melatonin in combination as a plant drought resistance agent, in particular to a new application of sodium hydrosulfide (NaHS) aqueous solution and melatonin aqueous solution to enhance plant drought resistance and its use method. Background technique [0002] Drought and water shortage are global problems currently restricting agricultural production. There are more than 1.4 billion hectares of arable land in the world, of which 600 million hectares are located in arid and semi-arid areas, accounting for about 43%. In my country, the area of ​​arid and semi-arid land accounts for about 48% of the total cultivated land area. Therefore, efficient use of water resources is imperative, and the development and promotion of modern drought-resistant technologies is becoming more and more important. That is, the development of new plant water-saving and drought-resistant agents to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/02A01N43/38A01P21/00
CPCA01N43/38A01N59/02
Inventor 金竹萍王志清裴雁曦
Owner SHANXI UNIV
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