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Electrochemiluminescent reagent prepared from a macrocyclic compound and its application

A macrocyclic compound, electrochemical technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc., can solve the problems of high analysis cost, difficult sample preparation, cumbersome process, etc. To achieve the effect of wide detection range, enhanced concentration response range and simple operation

Active Publication Date: 2020-08-07
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, methods such as HPLC and HPLCMS are commonly used for its detection, but the sample preparation is difficult, the process is cumbersome, time-consuming, and the analysis cost is high, so a new method is needed to detect SUC

Method used

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  • Electrochemiluminescent reagent prepared from a macrocyclic compound and its application
  • Electrochemiluminescent reagent prepared from a macrocyclic compound and its application
  • Electrochemiluminescent reagent prepared from a macrocyclic compound and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: The electrochemiluminescence reagent prepared by a kind of macrocyclic compound is that the molecular formula is C 36 h 30 N 6 o 15 , its structural formula is as attached figure 1 The shown macrocyclic compound is prepared by dissolving in an aqueous solution of a neutral electrolyte; the aqueous solution of a neutral electrolyte is a mixed solution containing 4-hydroxyethylpiperazineethanesulfonic acid and a hydrogen peroxide concentration of 0.1mol / L, adding The NaOH solution with a concentration of 0.1mol / L adjusts the pH value to neutral; the concentration of the macrocyclic compound in the electrochemiluminescence reagent is 2×10 -4 mol / L.

[0026] Observation group: with Al 2 o 3 Polish the glassy carbon electrode with polishing powder, wash it, and dry it to obtain a clean electrode. Measure 2ml of the electrochemiluminescence reagent prepared in Example 1 in the electrolytic cell, adopt a conventional three-electrode system, use platinum wir...

Embodiment 2

[0028] Example 2: The application of the electrochemiluminescent reagent prepared from the above-mentioned macrocyclic compound is the detection of succinylcholine chloride. The specific method is as follows:

[0029] 1) Prepare a mixed solution containing 4-hydroxyethylpiperazineethanesulfonic acid and hydrogen peroxide at a concentration of 0.1mol / L, add a NaOH solution with a concentration of 0.1mol / L to adjust the pH to neutral, and obtain a neutral electrolyte solution; ;

[0030] 2) Dissolve succinic acetylcholine chloride in a neutral electrolyte solution to prepare a concentration of 2.0×10 -2 The succinic acetylcholine chloride solution of mol / L;

[0031] 3) Take the electrochemiluminescent reagent and dilute it until the concentration of the macrocyclic compound is 1.0×10 -8 mol / L, then the succinylcholine chloride solution prepared in step 2) is added to the diluted electrochemiluminescence reagent to obtain a mixed solution, and water is added to adjust the conc...

Embodiment 3

[0034] Example 3: The application of the electrochemiluminescence reagent prepared from the above macrocyclic compound is the detection of succinylcholine chloride. The specific method is as follows:

[0035] 1) Prepare a mixed solution containing 4-hydroxyethylpiperazineethanesulfonic acid and hydrogen peroxide at a concentration of 0.1mol / L, add a NaOH solution with a concentration of 0.1mol / L to adjust the pH to neutral, and obtain a neutral electrolyte solution; ;

[0036] 2) Dissolve succinic acetylcholine chloride in a neutral electrolyte solution to prepare a concentration of 2.0×10 -2 The succinic acetylcholine chloride solution of mol / L;

[0037] 3) Take the electrochemiluminescent reagent and dilute it until the concentration of the macrocyclic compound is 1.0×10 -8 mol / L, then the succinylcholine chloride solution prepared in step 2) is added to the diluted electrochemiluminescence reagent to obtain a mixed solution, and water is added to adjust the concentration...

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Abstract

The invention discloses an electrochemiluminescence reagent prepared from a macrocyclic compound and application of the electrochemiluminescence reagent. The electrochemiluminescence reagent is prepared by dissolving the macrocyclic compound with the molecular formula of C36H30N6O15 which the structural formula is as shown in the figure I in the specification in a neutral electrolyte aqueous solution. The application of the electrochemiluminescence reagent comprises detection of succinylacetylcholine chloride. The electrochemiluminescence reagent can be used for carrying out detection on the succinylacetylcholine chloride, a sample is easy to prepare, the process is simple, the consumed time is short, the cost is low, the detection range is wide, and the electrochemiluminescence reagent isa novel electrochemiluminescence substance.

Description

technical field [0001] The invention relates to an electrochemiluminescence reagent and its application, in particular to an electrochemiluminescence reagent prepared from a macrocyclic compound and its application. Background technique [0002] Electrochemiluminescence (ECL), also known as electrochemiluminescence, has become a new analytical method applied to biological and molecular trace analysis. It is a special chemiluminescence phenomenon produced by electrochemical redox reaction, and it is the product of the combination of chemiluminescence and electrochemistry. This technology combines the advantages of high sensitivity of luminescence analysis and controllability of electrochemical point, and has a unique new advantage, which can detect luminescence intensity and current at the same time, has strong versatility, simple instrument, good time and space controllability It is a new and efficient detection method. In recent years, it has gradually become a research h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N27/26
CPCG01N21/76G01N27/26
Inventor 丛航邱菲赵永毅
Owner GUIZHOU UNIV
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