A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology

A layer-by-layer self-assembly and vaccine preparation technology, applied in the field of vaccine preparation, can solve the problems of uncontrollable virus inactivation rate, complex components, and low effective content

Active Publication Date: 2021-04-09
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Traditional vaccines are often developed due to clinical experience, and their preparation and vaccination methods have achieved great success in clinical practice, but there are problems such as residual toxicity of the vaccine, uncontrollable virus inactivation rate, complex components, and low effective content.

Method used

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  • A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology
  • A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology
  • A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Precision vaccine preparation method based on soft etching and layer self-assembly technology, including steps:

[0024](1) The polydimethylsiloxane polymer seal substrate is soaked in a concentration of 0.1% to a DNA adjuvant aqueous solution and a polypeptide anti-stock solution, each soaking time is 10-15 minutes, according to the polypeptide in aqueous solution The charge characteristics, moderate modification of the polypeptide (nine polycrystine molecules to the polypeptide molecule end) such that the polypeptide can be charged. The amount of the polymer seal in the adjuvant, the number of times in the antigen solution depends on the amount of the components required in the vaccine, the more the amount of the immersion is increased accordingly.

[0025](2) Blow the polymer seal by nitrogen in a sterile environment.

[0026](3) Print the polymer seal to a smooth substrate surface coated with a 10% polyvinyl alcohol film, thereby transferring the multilayer vaccine in the seal to t...

Embodiment 2

[0030]Precision vaccine preparation method based on soft etching and layer self-assembly technology, including steps:

[0031](1) The polydimethylsiloxane polymer seal substrate is soaked in a concentration of 0.001% in a DNA adjuvant aqueous solution and a polypeptide anti-stock solution, each soaking time is 10-15 minutes, according to the polypeptide in aqueous solution. The charge characteristics, moderate modification of the polypeptide (nine polycrystine molecules to the polypeptide molecule end) such that the polypeptide can be charged. The seal of the polymer seal is in the adjuvant, the number of soaking times in the antigen solution depends on the amount of components required in the vaccine.

[0032](2) Blow the polymer seal by nitrogen in a sterile environment.

[0033](3) Print the polymer seal to a smooth substrate surface coated with a 15% polyvinyl alcohol film, thereby transferring the multilayer vaccine microbial structure on the seal to the thin layer of polyvinyl alcohol....

Embodiment 3

[0037]Precision vaccine preparation method based on soft etching and layer self-assembly technology, including steps:

[0038](1) The polydimethylsiloxane polymer seal substrate is soaked in a concentration of 0.05% in an aqueous solution of the RNA adjuvant aqueous solution and the peptide anti-stock solution, each soaking time is 15 minutes, according to the charge characteristics of the polypeptide in aqueous solution Moderate modification of the polypeptide (nine polycrystine molecules to the polypeptide molecule) such that the polypeptide can be charged. The seal of the polymer seal is in the adjuvant, the number of soaking times in the antigen solution depends on the amount of components required in the vaccine.

[0039](2) Blow the polymer seal by nitrogen in a sterile environment.

[0040](3) Print the polymer seal to a smooth substrate surface coated with a 5% polyvinyl alcohol film, thereby transferring the multilayer vaccine microbial structure on the seal to the thin layer of pol...

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PUM

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Abstract

The invention belongs to the field of vaccine preparation, and relates to a precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology. The layer-by-layer self-assembly technology can accurately control the quantity and composition of each component, so in the field of precision vaccine preparation Has broad prospects. In the process of vaccine design, the ratio and quantity of various vaccine components can be controlled according to the type of vaccine required, thereby reducing or controlling the problem of inaccuracy existing in traditional vaccines. In addition, the size and shape of the prepared vaccine particles can be precisely controlled by means of soft etching printing technology. Thereby reducing the problem of uneven performance of vaccine particles.

Description

Technical field[0001]The present invention belongs to the field of the vaccine, and the method of preparation of vaccines, and is specifically involved in a preparation method of accurate vaccine based on soft etching and layer self-assembly techniques.Background technique[0002]Traditional vaccines often develop due to clinical experience, the preparation and vaccination methods have achieved huge success in clinical practice, but there is a problem that the vaccine has a certain toxicity, the viral inactivation rate is uncontrollable, the ingredient is complex, and the effective content is low. waiting to be solved.Inventive content[0003]In order to solve the above problems, the present invention provides a preparation method based on soft etching and layer self-assembly techniques, which can accurately control the number and composition of each component, reduce or control the disccision of conventional vaccines.[0004]In order to achieve the above object, the present invention ado...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K39/00
CPCA61K9/19A61K39/00
Inventor 张佩佩
Owner BEIHANG UNIV
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