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Precision vaccine preparation method based on soft etching and layer-by-layer self-assembly

A technology of layer-by-layer self-assembly and vaccine preparation, applied in the field of vaccine preparation, can solve the problems of low effective content, uncontrollable virus inactivation rate, complex components, etc., and achieve the effect of reducing uneven performance

Active Publication Date: 2018-11-30
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Traditional vaccines are often developed due to clinical experience, and their preparation and vaccination methods have achieved great success in clinical practice, but there are problems such as residual toxicity of the vaccine, uncontrollable virus inactivation rate, complex components, and low effective content.

Method used

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  • Precision vaccine preparation method based on soft etching and layer-by-layer self-assembly
  • Precision vaccine preparation method based on soft etching and layer-by-layer self-assembly
  • Precision vaccine preparation method based on soft etching and layer-by-layer self-assembly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology, including steps:

[0024] (1) Soak the polydimethylsiloxane polymer stamp substrate in a 0.1% DNA adjuvant aqueous solution and a polypeptide antigen solution in turn, each soaking time is 10-15 minutes, depending on the concentration of the polypeptide in the aqueous solution Charged properties, the polypeptide needs to be moderately modified (linking 9 polyarginine molecules to the end of the polypeptide molecule), so that the polypeptide can be positively charged. The times of immersion of the polymer stamp in the adjuvant and antigen solution depend on the amount of each component required in the vaccine, the more the required amount, the more times of immersion.

[0025] (2) Blow dry the polymer stamp with nitrogen in a sterile environment.

[0026] (3) The polymer stamp was printed on the surface of a smooth substrate coated with 10% polyvinyl alcohol film, so ...

Embodiment 2

[0030] A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology, including steps:

[0031] (1) Soak the polydimethylsiloxane polymer stamp substrate in the DNA adjuvant aqueous solution and the polypeptide antigen solution in turn at a concentration of 0.001%, each soaking time is 10-15 minutes, according to the concentration of the polypeptide in the aqueous solution Charged properties, the polypeptide needs to be moderately modified (linking 9 polyarginine molecules to the end of the polypeptide molecule), so that the polypeptide can be positively charged. The number of immersion times of the polymer stamp in the adjuvant and antigen solution depends on the amount of each component required in the vaccine.

[0032] (2) Blow dry the polymer stamp with nitrogen in a sterile environment.

[0033] (3) The polymer stamp was printed on the surface of a smooth substrate coated with 15% polyvinyl alcohol film, so that the multi-layer va...

Embodiment 3

[0037] A precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology, including steps:

[0038] (1) Soak the polydimethylsiloxane polymer stamp substrate in an aqueous solution of RNA adjuvant with a concentration of 0.05% and a polypeptide antigen solution in turn, each soaking time is 15 minutes, according to the charging characteristics of the polypeptide in the aqueous solution , the polypeptide needs to be moderately modified (9 polyarginine molecules are linked to the end of the polypeptide molecule), so that the polypeptide can be positively charged. The number of immersion times of the polymer stamp in the adjuvant and antigen solution depends on the amount of each component required in the vaccine.

[0039] (2) Blow dry the polymer stamp with nitrogen in a sterile environment.

[0040] (3) The polymer stamp was printed on the surface of a smooth substrate coated with 5% polyvinyl alcohol film, so that the multi-layer vaccine m...

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Abstract

The invention belongs to the field of preparation of vaccines, and relates to a precision vaccine preparation method based on soft etching and layer-by-layer self-assembly. According to the present invention, the amounts of each component and the composition can be precisely controlled by using the layer-by-layer self-assembly technology, such that the method has wide prospects in the field of thepreparation of precision vaccines; during the design of the vaccine, the ratio and the amounts of each vaccine component can be controlled according to the type of the required vaccine, such that theproblem that the traditional vaccine is not precise can be reduced or controlled; and by using the soft etching printing technology, the sizes and the shapes of the prepared vaccine particles can beprecisely controlled so as to reduce the problem of the non-uniform performance of the vaccine particles.

Description

technical field [0001] The invention belongs to the field of vaccine preparation, and relates to a vaccine preparation method, in particular to a precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology. Background technique [0002] Traditional vaccines are often developed due to clinical experience, and their preparation and vaccination methods have achieved great clinical success, but there are problems such as residual toxicity of the vaccine, uncontrollable virus inactivation rate, complex components, and low effective content. waiting to be solved. Contents of the invention [0003] In order to solve the above problems, the present invention provides a precise vaccine preparation method based on soft etching and layer-by-layer self-assembly technology, which can precisely control the quantity and composition of each component, and reduce or control the problem of inaccuracy existing in traditional vaccines. [0004] In ord...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/19A61K39/00
CPCA61K9/19A61K39/00
Inventor 张佩佩
Owner BEIHANG UNIV
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