Ultrathin carrier cell sheet and preparation method thereof

Abstract

The invention discloses an ultrathin carrier cell sheet and a preparation method thereof. The preparation method comprises the following steps of (1) spreading a gel material solution onto a substrate, and condensing into a solid state; (2) generating an ion polymer layer which has opposite polarity with the gel material on the substrate spread with the gel material, generating an ion polymer layer which has opposite polarity with the upper ion polymer layer, on the upper ion polymer layer, and repeating the operation, so as to form a layered self-assembly film; (3) planting a target cell into the substrate obtained in step (2), and conventionally culturing; (4) in the culture process, enabling the layered self-assembled film loaded with the cell to disengage from the substrate, so as to form the cell sheet. Compared with the traditional preparation method of the cell sheet, the preparation method has the advantages that the character control or decomposing step of a supporter material is not needed, the mechanical strength of the cell sheet is obviously improved, and macromolecular medicines, such as protein, polypeptide, antibody and nucleic acid, can be loaded.

Description

technical field [0001] The invention belongs to the field of tissue engineering, in particular to an ultra-thin carrier cell sheet and a preparation method thereof. Background technique [0002] Cell transplantation is widely used in the field of tissue engineering for cornea, myocardial reconstruction, and tissue repair. However, the traditional method of injecting cell suspension cannot ensure the effective residence of cells in the damaged area, nor can it control the shape and size of the repaired area. Moreover, since the cell suspension needs to be treated with trypsin or chemicals during the collection process, it can be stripped and recovered from the surface of the culture container, which will cause cell damage and damage the original function. Therefore, cells are seeded onto biodegradable scaffold materials such as collagen gel and fibrin gel, and then transplanted together with the scaffold material. This cell-containing scaffold has tunable mechanical propert...

AI Technical Summary

Problems solved by technology

However, the cell sheets constructed by these methods connect the cells to each other through the structure between cells and the extracellular matrix (extracellular mix, ECM) secreted by cells, so the mechanical properties of this cell sheet are poor and easy to break

And, because there is no additional material structure, such cell sheets cannot be loaded with drugs or further artificially modified

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